FY 2003 Columbia Estuary proposal 30012

Additional documents

TitleType
30012 Narrative Narrative

Section 1. Administrative

Proposal titleCompare Bacterial Fish Pathogen Populations in Hatchery Water and in Adjacent Creek Water and Evaluate Possible Disease Transfer Between Them.
Proposal ID30012
OrganizationU.S. Fish and Wildlife Service (USFWS)
Proposal contact person or principal investigator
NamePeter W. Taylor
Mailing address1440 Abernathy Creek Rd. Longview, WA 98632
Phone / email3604256072 / pete_taylor@fws.gov
Manager authorizing this projectCarl V. Burger, Center Director, AFTC
Review cycleColumbia Estuary
Province / SubbasinColumbia Estuary / Elochoman
Short descriptionDetermine the presence of bacterial fish pathogens within a hatchery water system and in the waters of an adjacent creek used as part of the hatchery water supply. Determine the potential for pathogen transfer between the two water systems.
Target speciescoho, steelhead, cutthroat
Project location
LatitudeLongitudeDescription
46.22 -123.1375 Abernathy Creek
Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA
Action 8
Action 141
Action 184
Action 195

Relevant RPAs based on NMFS/BPA review:

Reviewing agencyAction #BiOp AgencyDescription
NMFS Action 184 NMFS The Action Agencies and NMFS shall work within regional prioritization and congressional appropriation processes to establish and provide the appropriate level of FCRPS funding for a hatchery research, monitoring, and evaluation program consisting of studies to determine whether hatchery reforms reduce the risk of extinction for Columbia River basin salmonids and whether conservation hatcheries contribute to recovery.
BPA Action 141 NMFS The Action Agencies shall evaluate juvenile fish condition due to disease in relation to high temperature impacts during critical migration periods. This evaluation should include monitoring summer migrants at lower Columbia and lower Snake river dams to clarify the possible link between temperature and fish disease and mortality. This information will be used to assess the long-term impacts of water temperature on juvenile fish survival.
BPA Action 184 NMFS The Action Agencies and NMFS shall work within regional prioritization and congressional appropriation processes to establish and provide the appropriate level of FCRPS funding for a hatchery research, monitoring, and evaluation program consisting of studies to determine whether hatchery reforms reduce the risk of extinction for Columbia River basin salmonids and whether conservation hatcheries contribute to recovery.

Section 2. Past accomplishments

YearAccomplishment
1998 Completed a pilot study on the distribution of fish pathogens in hatchery environments
2001 Developed a nested PCR for the detection of three bacterial fish pathogens, manuscript submitted to peer-reviewed journal

Section 3. Relationships to other projects

Project IDTitleDescription
RPA Action #8 This project may provide a method for risk assesment for hatcheries
RPA 141 This project may provide additional information on fish pathogens
RPA 184 This project may provide monitoring and assesment of hatchery fish health
RPA 195 This project may add to knowledge on juvenile salmonid mortalities.

Section 4. Budget for Planning and Design phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
1. Selection of sampling sites a. stream site survey b. hatchery site survey 1 $1,000
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Construction and Implementation phase

Section 6. Budget for Operations and Maintenance phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
1. Determine presence and distribution of fish pathogens. a. water sample collection and DNA extraction 2 $27,726
b. fish sample collection and DNA extraction 2 $27,726
c. PCR assays and record 2 $15,226
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
1a - c. Water sample collection, fish collection, PCR analysis 2003 2004 $38,487
Outyear budgets for Monitoring and Evaluation phase
FY 2004
$34,487

Section 8. Estimated budget summary

Itemized budget
ItemNoteFY 2003 cost
Personnel FTE: GS-5 lab technician $25,544
Fringe $8,940
Supplies Centrovac DNA concentrator($8,000) $16,999
Travel $2,000
Indirect $18,195
$71,678
Total estimated budget
Total FY 2003 cost$71,678
Amount anticipated from previously committed BPA funds$0
Total FY 2003 budget request$71,678
FY 2003 forecast from 2002$0
% change from forecast0.0%
Cost sharing
OrganizationItem or service providedAmountCash or in-kind
USFWS senior researcher, GS-12, 0.2 FTE $18,000 in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

Recommendation:
Do not fund - no response required
Date:
Mar 1, 2002

Comment:

Not fundable. The proposal is inadequate for technical review. It is unclear how samples of Abernathy creek will be taken with respect to seasonal fluctuations in abundance of fish and seasonal fluctuations of the physical environment. The statistical methods are not adequately explained. The qualifications of the investigator are not described. A thorough proposal for similar work has been made in the Columbia Cascade subbasin.
Recommendation:
Recommended Action
Date:
May 17, 2002

Comment:

This project may be better evaluated in the Mainstem and Systemwide Province.
Recommendation:
Do Not Fund
Date:
Jun 7, 2002

Comment:

Not fundable. The proposal is inadequate for technical review. It is unclear how samples of Abernathy creek will be taken with respect to seasonal fluctuations in abundance of fish and seasonal fluctuations of the physical environment. The statistical methods are not adequately explained. The qualifications of the investigator are not described. A thorough proposal for similar work has been made in the Columbia Cascade subbasin.
Recommendation:
Date:
Jul 19, 2002

Comment:

Statement of Potential Biological Benefit to ESU
Project will evaluate the pathogen presence and distribution to determine critical control points for minimizing the transfer of these pathogens either into or out of the hatchery system.

Comments
The proposed research has the potential to answer a number of important questions on the transmission of disease between wild and hatchery stock. However, the proposal as presented lacks adequate detail.

Already ESA Req? No

Biop? No


Recommendation:
C
Date:
Jul 23, 2002

Comment:

Defer to development of RM&E plan to identify research priorities (has some overlaps with 30003, 3008, and 30013); see comment on proposal number 30003.
Recommendation:
Do Not Fund
Date:
Oct 30, 2002

Comment: