FY 2002 Columbia Plateau proposal 25052
Contents
Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary
Reviews and Recommendations
Additional documents
| Title | Type |
|---|---|
| 25052 Narrative | Narrative |
| 25052 Sponsor Response to the ISRP | Response |
| 25052 Powerpoint Presentation | Powerpoint Presentation |
| Columbia Plateau: Columbia Lower Middle Subbasin Map with BPA Fish & Wildlife Projects | Subbasin Map |
| Columbia Plateau: Columbia Lower Middle Subbasin Map with BPA Fish & Wildlife Projects | Subbasin Map |
| Letter from A. Maule (USGS) to CBFWA Re: Within-year modification request for project 25052 | Correspondence |
Section 1. Administrative
| Proposal title | Sex Reversal in Hanford Reach Fall Chinook Salmon |
| Proposal ID | 25052 |
| Organization | Columbia River Research Lab USGS-BRD (CRRL) |
| Proposal contact person or principal investigator | |
| Name | Matt Mesa |
| Mailing address | 5501 A Cook-Underwood Rd Cook, WA 98605 |
| Phone / email | 5095382299 / matt_mesa@usgs.gov |
| Manager authorizing this project | James Seelye, Director |
| Review cycle | Columbia Plateau |
| Province / Subbasin | Columbia Plateau / Mainstem Columbia |
| Short description | We will determine if the prevalence of male specific genetic markers in juvenile fall chinook salmon in the Hanford Reach is consistent with phenotype, and whether this evidence of sexual disruption is associated with biomarkers of contaminant exposure |
| Target species | fall chinook salmon (Oncorhynchus tshawytscha); |
Project location
| Latitude | Longitude | Description |
|---|---|---|
| 46.6708 | -119.4325 | Hanford Reach |
Reasonable and Prudent Alternatives (RPAs)
Sponsor-reported:
| RPA |
|---|
Relevant RPAs based on NMFS/BPA review:
| Reviewing agency | Action # | BiOp Agency | Description |
|---|
Section 2. Past accomplishments
| Year | Accomplishment |
|---|
Section 3. Relationships to other projects
| Project ID | Title | Description |
|---|---|---|
| Genetic sex of chinook salmon in the Columbia River Basin | J. Nagler (PI); project will look at genotype in other fish in the Hanford Reach. |
Section 4. Budget for Planning and Design phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2002 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Planning and Design phase
Section 5. Budget for Construction and Implementation phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2002 cost | Subcontractor |
|---|---|---|---|---|
| Objective 1 - Determine the levels of biomarkers of estrogen-specific pollution, and the activity of physiological indicators of contaminant exposure in Hanford Reach juvenile fall chinook salmon. | Task 1a- Collect and sample 200 juvenile fall chinook salmon from the Hanford Reach and 200 up-river bright fall chinook salmon from the Little White Salmon National Fish Hatchery (LWSNFH). | FY02 | $35,322 | |
| Task 1b- Determine the levels of the estrogen responsive biomarkers Vg and zrp in Hanford Reach and LWSNFH juvenile fall chinook salmon | FY02-FY03 | $28,465 | ||
| Task 1c- Determine the activity of total cytochrome P-450 (P-450) and ethoxyresofurin O-deethylase (EROD) enzymes in the liver of Hanford Reach and LWSNFH juvenile fall chinook salmon. | FY02-FY03 | $21,445 | ||
| Task 1d- Determine the levels of malondialdehyde (an indicator of lipid peroxidation) in liver preparations from Hanford Reach and LWSNFH juvenile fall chinook salmon | FY02-FY03 | $18,298 | ||
| Objective 2 – Determine the incidence of intersexuality in Hanford Reach juvenile fall chinook salmon by histological analysis of gonadal tissue. | Task 2a- Gonad samples from juvenile fall chinook (collected in Task 1a) will be processed using standard histological techniques. | FY02-FY03 | $25,309 | |
| Objective 3- Determine the phenotypic sex ratio of Hanford Reach or LWSNFH juvenile fall chinook salmon by examination of the gonads | Task 3a- Collect 750 juvenile fall chinook salmon from the Hanford Reach and 750 up-river bright fall chinook salmon from the LWSNFH | FY02 | $38,179 | |
| Task 3b- Rear Hanford Reach and LWSNFH juvenile fall chinook salmon and sample them when they are at least 120 mm fork length. | FY02 | $18,032 | ||
| Task 3c- Determine the gonadal sex of Hanford Reach and LWSNFH juvenile fall chinook salmon | FY02-FY03 | $17,079 | ||
| Objective 4 - Determine the genotypic sex ratio of Hanford reach juvenile fall chinook salmon. | Task 4a- Determine the genetic sex ratio in juvenile fall chinook from the Hanford Reach and the LWSNFH | FY02-FY03 | $60,192 |
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|---|---|---|
| Objective 1, Task 1b (see above) | 2002 | 2003 | $21,853 |
| Task 1c (see above) | 2002 | 2003 | $31,884 |
| Task 1d (see above) | 2002 | 2003 | $28,936 |
| Objective 2, Task 2a (see above) | 2002 | 2003 | $26,993 |
| Objective 3, Task 3c (see above) | 2002 | 2003 | $37,270 |
| Objective 4, Task 4a (see above) | 2002 | 2003 | $6,103 |
Outyear budgets for Construction and Implementation phase
| FY 2003 |
|---|
| $153,038 |
Section 6. Budget for Operations and Maintenance phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2002 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Operations and Maintenance phase
Section 7. Budget for Monitoring and Evaluation phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2002 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Monitoring and Evaluation phase
Section 8. Estimated budget summary
Itemized budget
| Item | Note | FY 2002 cost |
|---|---|---|
| Personnel | FTE: 2.0 | $85,921 |
| Fringe | 30% | $25,776 |
| Supplies | $29,500 | |
| Travel | $19,778 | |
| Indirect | $65,826 | |
| Subcontractor | Dr. J Nagler, U of Idaho | $35,520 |
| $262,321 | ||
Total estimated budget
| Total FY 2002 cost | $262,321 |
| Amount anticipated from previously committed BPA funds | $0 |
| Total FY 2002 budget request | $262,321 |
| FY 2002 forecast from 2001 | $0 |
| % change from forecast | 0.0% |
Cost sharing
| Organization | Item or service provided | Amount | Cash or in-kind |
|---|---|---|---|
| University of Idaho | Salary & benefits | $5,000 | in-kind |
Reviews and recommendations
This information was not provided on the original proposals, but was generated during the review process.
Fundable only if response is adequate
Jun 15, 2001
Comment:
Fundable only if an adequate response is provided. This proposal addresses an important and disturbing phenomenon first brought to the ISRP's attention through the innovative proposal submission by Nagler, Dauble, and Thorgaard (#22013; Genetic sex of chinook salmon in the Columbia River Basin; PI = Nagler). The ISRP recognized the sex reversal problem in Hanford Reach fall Chinook as an important one, and recommended funding an initial examination of the extent of this problem as one of the two highest priority projects in the recent Innovative Competition. Council and BPA have approved funding for the innovative project.
The ISRP's review comments on the Innovative Proposal 22013 are shown immediately below in italics.
This is an innovative proposal because it addresses a newly recognized critical uncertainty in the Hanford Reach fall chinook stock and proposes to use a new genetic assay technique to do so. It is also a high priority project as it addresses a critical question about population genetic structure in the Hanford Reach and other chinook stocks.The authors' preliminary data show surprising evidence of sex-reversal (some genetic males are functional females) in Hanford-Reach-spawning wild chinook, apparently the result of some environmental insult (e.g., EDC's, exposure to pesticides). The data are intriguing and worrisome. Half the offspring of the sex-reversed fish will be normal males, but half will be YY males, capable of producing only sons, disproportionately increasing the ratio of males to females in the next generation, an accelerating increase if the sex-reversal continues in each generation. The effect would be a decreasing proportion of normal females and decreasing reproductive fitness, a serious barrier to recovery. It's clearly important to find out if other stocks of wild spawning chinook are affected, and it's important to find out if YY males are indeed present. The region needs to know the extent of the genetic sex reversal phenomenon.
Many of the positive comments and biological concerns stated in the review comments above also apply to this proposal (#25052). This proposal, while not directly linked to Project 22013, is related to it. The studies appear to complement each other, such that if this project were funded, it should be much more closely linked to project 22013 than is suggested in this proposal. The similarity between these two studies is that they will both examine juvenile fall chinook salmon from the Hanford Reach as a consequence of a reported incidence of a male-specific genetic marker in adult females from this population (Nagler et al. 2001). The funded project (22013) will look for incidence of a YY-genotype in wild juveniles over two seasons, while this proposal will examine the levels of biomarkers, phenotype and genotype, and incidence of intersex in juveniles.
The innovative proposal 22013 is restricted in scope as compared to this proposal, most likely to fit the funding and timeframe criteria of the innovative solicitation. The focus of the innovative proposal was to gather genetic and phenotypic data from Hanford Reach juvenile fall chinook to further corroborate or refute the preliminary observations of high levels of sex reversal and intersex individuals. That proposal infers, but does not outline a strategy to examine, that the genetic results could be related to higher levels of biocontamination from pollutants. This proposal (25052), in many ways, is the next logical step beyond the funded project 22013. Consequently, if funded, the two projects should be integrated more fully (note that Nagler serves as PI on 22013 and as a Co-PI on this proposal).
Specific questions: Can the assay be applied to phenotypic males and females ... if so the returning phenotypic sex ratio should be confirmed by sampling the genotypic ratio. It is very important to confirm that sex can be reversed equally between sexes ... male to female or visa versa. Should this wait for the Innovative Project to be completed? If not, why not?
Comment:
This project would compliment the ongoing project that was funded through the innovative category. Due to the limitations in the innovative funding category, the other study is not able to address the juvenile component of the sex reversal phenomenon. There are opportunities for cost savings of approximately $56k on this project through coordination with existing sampling programs for PIT tagged fish. Funding for this project should be delayed until the prior project is completed, therefore the budget has been adjusted to begin in FY03.Comment:
Fundable. The response adequately addressed the ISRP concern about potential duplication and coordination with Innovative Proposal 22013.This proposal addresses an important and disturbing phenomenon first brought to the ISRP's attention through the innovative proposal submission by Nagler, Dauble, and Thorgaard (#22013; Genetic sex of chinook salmon in the Columbia River Basin; PI = Nagler). The ISRP recognized the sex reversal problem in Hanford Reach fall Chinook as an important one, and recommended funding an initial examination of the extent of this problem as one of the two highest priority projects in the recent Innovative Competition. Council and BPA have approved funding for the innovative project.
The ISRP's review comments on the Innovative Proposal 22013 are shown immediately below in italics.
This is an innovative proposal because it addresses a newly recognized critical uncertainty in the Hanford Reach fall chinook stock and proposes to use a new genetic assay technique to do so. It is also a high priority project as it addresses a critical question about population genetic structure in the Hanford Reach and other chinook stocks.
The authors' preliminary data show surprising evidence of sex-reversal (some genetic males are functional females) in Hanford-Reach-spawning wild chinook, apparently the result of some environmental insult (e.g., EDC's, exposure to pesticides). The data are intriguing and worrisome. Half the offspring of the sex-reversed fish will be normal males, but half will be YY males, capable of producing only sons, disproportionately increasing the ratio of males to females in the next generation, an accelerating increase if the sex-reversal continues in each generation. The effect would be a decreasing proportion of normal females and decreasing reproductive fitness, a serious barrier to recovery. It's clearly important to find out if other stocks of wild spawning chinook are affected, and it's important to find out if YY males are indeed present. The region needs to know the extent of the genetic sex reversal phenomenon.
Many of the positive comments and biological concerns stated in the review comments above also apply to this proposal (#25052). This proposal, while not directly linked to Project 22013, is related to it. The studies complement each other. The similarity between these two studies is that they will both examine juvenile fall chinook salmon from the Hanford Reach as a consequence of a reported incidence of a male-specific genetic marker in adult females from this population (Nagler et al. 2001). The funded project (22013) will look for incidence of a YY-genotype in wild juveniles over two seasons, while this proposal will examine the levels of biomarkers, phenotype and genotype, and incidence of intersex in juveniles.
The innovative proposal 22013 is restricted in scope as compared to this proposal, most likely to fit the funding and timeframe criteria of the innovative solicitation. The focus of the innovative proposal was to gather genetic and phenotypic data from Hanford Reach juvenile fall chinook to further corroborate or refute the preliminary observations of high levels of sex reversal and intersex individuals. That proposal infers, but does not outline a strategy to examine, that the genetic results could be related to higher levels of biocontamination from pollutants. This proposal (25052), in many ways, is the next logical step beyond the funded project 22013.
See detailed ISRP comments on Hanford Reach projects
Comment:
Statement of Potential Biological Benefit to ESUN/A (for RPA ESUs)
Comments
Potential for future funding, wait until ongoing studies are completed or summarized.
Already ESA Req? N/A
Biop? no
Comment:
This proposal addresses the potential causes of possible sex reversal as a result of urban or agricultural pollution. WDFW does not thermally mark chinook at Priest Rapids Hatchery, so this is not a possible cause of the apparent sex reversals. This proposal is very closely related to the ongoing work of Nagler. Scientific review should determine if both projects are needed, or if the ongoing Nagler project is sufficient to address the issue.Comment:
Comment:
Comment:
We received no funds in FY03. If this project is to be conducted in FY04-FY05, these funds are needed. Alec G. Maule (USGS, Col. R. Res. Lab. alec_maule@usgs.gov) is PI for this project