FY 2002 Columbia Plateau proposal 200203700

Section 1. Administrative

Proposal titleCharacterize Genetic Differences and Distribution of Freshwater Mussels
Proposal ID200203700
OrganizationConfederated Tribes of the Umatilla Indian Reservation (CTUIR)
Proposal contact person or principal investigator
NameGary James
Mailing addressP.O. Box 638 Pendleton Oregon 97801
Phone / email5419662371 / garyjames@ctuir.com
Manager authorizing this projectGary James
Review cycleColumbia Plateau
Province / SubbasinColumbia Plateau / Umatilla
Short descriptionConduct freshwater mussel surveys to assess their status and test for geographical genetic differences among the western pearlshell mussel, Margaritifera falcata.
Target speciesFreshwater Mussels
Project location
LatitudeLongitudeDescription
Project ranges from the mouth of the Umatilla River to Headwaters
Headwaters of the Umatilla River Basin to the East
Headwaters of the Umatilla River Basin to the South
Headwaters of the Umatilla River Basin to the West
Entire Middle Fork John Day Basin
Middle Fork John Day River at Mouth
Middle Fork John Day River at Phipps Meadows
45.71 -118.34 Umatilla River
45.7318 -120.6499 John Day River
Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agencyAction #BiOp AgencyDescription

Section 2. Past accomplishments

YearAccomplishment
N/A New Project

Section 3. Relationships to other projects

Project IDTitleDescription
9000501 Umatilla Basin Natural Production Monitoring and Evaluation This project is part of the overall goal to recover an intact, fully functioning, salmonid producing river in the Umatilla River. The CTUIR has numerous projects focusing on recovery of the Umatilla River Basin for salmonids and other species, such as
8373600 Umatilla Passage Facility Operations and Maintenance Pacific lampreys. The restoration project for the Pacific lampreys has the closest relationship to this project, because both focus on restoration of species that require healthly salmon populations for their persistence.
8802200 Umatilla Fish Passage Operations
9506000 Pacific Lamprey Research and Restoration
8710001 Umatilla Fish Habitat Enhancement

Section 4. Budget for Planning and Design phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Construction and Implementation phase

Section 6. Budget for Operations and Maintenance phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2002 costSubcontractor
1. Assess the status of freshwater mussels in the Umatilla and Middle Fork John Day rivers a. Determine sampling locations. 1 $8,000
1. b. Conduct mussel surveys 5 $203,977
1. c. Analyze data 3 $5,000
2. Test for genetic differences a. Collect samples 3 $5,000
2. b. Develop microsatellite DNA primers ($2500/primer x 8 primers) 3 $24,700 Yes
2. c. Characterize microsatellite DNA genotypes ($50/fish x 60 fish/samples x 6 samples) 6 $22,230 Yes
2. d. Develop mitochondial DNA primers $20,000 Yes
2. e. Characterize mitochondial DNA variation $15,000 Yes
2. f. Analyze results (0.05 FTE) 2 $3,000
3. Complete final report to funding agency a. Write final report (0.05 FTE) 2 $3,000
4. Publish results in scientific journal a. Submit manuscript for review (page charges @ $100/page + reprints) 1 $2,000
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
1. Assess the status of freshwater mussels in the Umatilla and Middle Fork John Day rivers 2003 2006 $867,908
2. Test for genetic differences 2003 2003 $86,930
3. Complete final report to funding agency 2003 2006 $24,000
4. Publish results in scientific journal 2003 2006 $8,000
Outyear budgets for Monitoring and Evaluation phase
FY 2003FY 2004FY 2005FY 2006
$343,097$377,406$415,146$456,660

Section 8. Estimated budget summary

Itemized budget
ItemNoteFY 2002 cost
Personnel FTE: 1.0 project leader, 1.0 Fish Bio II, 1.0 Fish Tech, 0.5 fish techs (temps) $117,600
Fringe 30% full-time; 19% temps $34,488
Supplies 6 wet suits and gear @$500/person, 4 hand held GPS units @ $350/each, Laser level @ $2000,Field mat. $7,000
Travel Lease vehicles x 2, site visits per diem $20,000
Indirect 34% of personnel, supplies and travel $60,889
Capital $0
NEPA $0
PIT tags $0
Subcontractor Genetics Laboratory $71,930
Other $0
$311,907
Total estimated budget
Total FY 2002 cost$311,907
Amount anticipated from previously committed BPA funds$0
Total FY 2002 budget request$311,907
FY 2002 forecast from 2001$0
% change from forecast0.0%
Cost sharing
OrganizationItem or service providedAmountCash or in-kind
Northwest Indian Fisheries Commission 0.10 FTE for Dr. Kenneth Currens $6,000 in-kind
U.S. Forest Service 0.24 for John Sanchez and USFS techs. $7,000 in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

Recommendation:
Fundable only if response is adequate
Date:
Jun 15, 2001

Comment:

Fundable if adequate responses are given to ISRP concerns. They should discuss focusing goals and objectives on practical applications. What are the limiting factors on mussels? Food supply? What life history stage is limited? How might abundance be increased?

The proposal was well-prepared and well-presented. PI's look very qualified for the type of work proposed. Objectives are straightforward, well-described as are the associated tasks. Good linkages to regional planning documents, FWP, and to general ecosystem principles. While these are commendable in an academic sort of approach to obtaining basic information about mussels, information which might have importance in management decisions, the primary goal of the project to restore harvestable populations of mussels should not be obscured. It would be well to enlarge upon the tasks, and evaluation of results that relate directly to this goal.

One of the attractive aspects of the proposal is the planned genetics work at the regional level, which will survey genetic variation among mussel populations throughout the Columbia River basin. We note that one possible outcome, as discussed in the text (p. 4), is that the populations will be found to be undifferentiated. A survey at this scale (as is also proposed for Pacific lamprey) will likely provide important information that will bear on decisions about management units, reintroduction efforts, supplementation efforts (if they are initiated), and population structure.


Recommendation:
High Priority
Date:
Aug 3, 2001

Comment:

Historically, freshwater mussels were an important subsistence species for the CTUIR. However, mussel populations have declined and as a result mussels can no longer be used for purposes of subsistence. Mussels have been listed as candidate species in the Willamette River. However, little, if anything, is known about freshwater mussel distribution, abundance and habitat quality east of the Cascades. The ODFW suggests that there is a need to initiate this type of work. The reviewers recommend that preliminary genetic analyses should be limited to mtDNA (RFLPs) analyses. Microsatellite analyses should only be used if mtDNA data are not conclusive.
Recommendation:
Fund
Date:
Aug 10, 2001

Comment:

Fundable in part to do the distribution work. The response was too brief and addressed the ISRP concerns superficially. The reviewers were not convinced that this was the right approach to addressing mussel issues. The reviewers recommend that the distribution work be done with a solid experimental design testing several hypotheses including fish presence, sedimentation, habitat degradation, and overexploitation. The genetic work, while well-designed and appropriate to test whether one or multiple populations exist, can be conducted at a later date after the distribution and ecological hypothesis testing are complete. Because of the expected low abundance of mussels, however, tissue samples should be collected throughout the study as populations are encountered.

The proposed study, while thorough, seems to be one of relatively high-cost asking for nearly $2 million over its proposed 5-year duration. It is worth asking if the major objectives of the study can be achieved with a lesser amount and a shorter study duration?


Recommendation:
Date:
Oct 1, 2001

Comment:

Statement of Potential Biological Benefit to ESU
N/A

Comments

Already ESA Req? N/A

Biop? no


Recommendation:
Fund
Date:
Jan 3, 2002

Comment:

Characterize genetic differences and distribution of freshwater mussels, Project 25093

ISRP provided a Fund In Part recommendation for the distribution work submitted in Proposal 25093. CBFWA rated the entire project as High Priority. Thus, the Council has a consensus recommendation for the distribution portion of the proposal.

Staff Recommendation: Freshwater mussels were an important subsistence species for the CTUIR. Given the cultural significance of the freshwater mussels and the lack of any knowledge base as to their population levels and distribution, staff recommends funding the distribution work proposed in the study.

Budget effect on base program (Project 25093):

FY 2002 FY 2003 FY 2004
Increase $220,000 Increase $228,000 Increase $237,000

Recommendation:
Fund
Date:
Mar 6, 2002

Comment:


Recommendation:
Fund
Date:
Sep 20, 2003

Comment:

Delayed start, requesting modification to spend out balance for this year. 05 represents a 3.4% increase for COLA.
Recommendation:
Date:
Sep 20, 2003

Comment:

In FY 03 CTUIR is seeking an increase of $9,509 to cover previous years of unfunded increases in Indirect rates. FY 03 Sponsor Recommended level would increase to from $228,000 to $237,509. Previous year budgets were submitted with a 34% Indirect rate because the Department of Interior hadn't yet issued new approved rates for BPA to consider. Unfunded 2002 Indirect Rate Increase @ 37.2% = $418. Unfunded 2003 Indirect Rate Increase @ 39.64% = $9,027.
REVIEW:
NW Power and Conservation Council's FY 2006 Project Funding Review
Funding category:
expense
Date:
May 2005
FY05 NPCC start of year:FY06 NPCC staff preliminary:FY06 NPCC July draft start of year:
$237,000 $237,000 $237,000

Sponsor comments: See comment at Council's website