Section 1. Administrative
| Proposal title | Compare Bacterial Fish Pathogen Populations in Hatchery Water and in Adjacent Creek Water and Evaluate Possibile Disease Transfer Between Them. |
| Proposal ID | 200311200 |
| Organization | U.S. Fish and Wildlife Service (USFWS) |
| Proposal contact person or principal investigator |
| Name | Dr. Peter W. Taylor |
| Mailing address | 1440 Abernathy Creek Rd Longview, WA 98632 |
| Phone / email | 3604256072 / pete_taylor@fws.gov |
| Manager authorizing this project | Carl Burger |
| Review cycle | Mainstem/Systemwide |
| Province / Subbasin | Mainstem/Systemwide / |
| Short description | Determine the presence of bacterial fish pathogens within a hatchery water system and in the waters of an adjacent creek used as part of the hatchery water supply. Determine the potential for pathogen transfer between the two water systems. |
| Target species | coho, steelhead, cutthroat |
Project location
| Latitude | Longitude | Description |
|---|
| 46.22 |
-123.1375 |
Abernathy Fish Technology Center |
Reasonable and Prudent Alternatives (RPAs)
Relevant RPAs based on NMFS/BPA review:
| Reviewing agency | Action # | BiOp Agency | Description |
|---|
Section 2. Past accomplishments
| Year | Accomplishment |
|---|
| 1998 |
Completed a pilot study on the distribution of fish pathogens in hatchery environments |
| 2001 |
Developed a nested PCR for the detection of three fish pathogens, manuscript in press. |
Section 3. Relationships to other projects
| Project ID | Title | Description |
|---|
Section 4. Budget for Planning and Design phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
|---|
| 1. Selection of sampling sites |
a. stream site surveyb. hatchery site survey |
1 |
$1,000 |
|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Planning and Design phase
Section 5. Budget for Construction and Implementation phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Construction and Implementation phase
Section 6. Budget for Operations and Maintenance phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Operations and Maintenance phase
Section 7. Budget for Monitoring and Evaluation phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
|---|
| 1. Determine presence and distribution of fish pathogens. |
a. water sample collection and DNA extraction |
2 |
$27,726 |
|
|
b. fish sample collection and DNA extraction |
2 |
$27,726 |
|
|
c. PCR assays and record |
2 |
$15,226 |
|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
| 1a - c. Water sample collection, fish collection, PCR analysis |
2003 |
2004 |
$38,487 |
Outyear budgets for Monitoring and Evaluation phase
Section 8. Estimated budget summary
Itemized budget
| Item | Note | FY 2003 cost |
|---|
| Personnel |
FTE: GS-5 lab technician |
$25,544 |
| Fringe |
|
$8,940 |
| Supplies |
Centrovac DNA concentrator($8,000) |
$16,999 |
| Travel |
|
$2,000 |
| Indirect |
|
$18,195 |
| $71,678 |
Total estimated budget
| Total FY 2003 cost | $71,678 |
| Amount anticipated from previously committed BPA funds | $0 |
| Total FY 2003 budget request | $71,678 |
| FY 2003 forecast from 2002 | $0 |
| % change from forecast | 0.0% |
Cost sharing
| Organization | Item or service provided | Amount | Cash or in-kind |
|---|
| USFWS |
senior researcher, GS-12, 0.2 FTE |
$18,000 |
in-kind |
Reviews and recommendations
This information was not provided on the original proposals, but was generated during the review process.
Recommendation:
Do not fund - no response required
Date:
Aug 2, 2002
Comment:
This proposal would investigate the possible exchange, between hatcheries and the environment, of two of the most serious bacterial diseases found in salmonid hatcheries of the Pacific Northwest, Bacterial Cold Water disease and Furunculosis, caused by Flavobacterium psychrophilum and Aeromonas salmonicida. Coho and steelhead are most susceptible but other salmonid species can be infected or act as carriers. These diseases are not limited to hatchery fish but also occur among wild populations. The proposed work could complement a similar proposal (#35039, USGS-CRRL) but the content of this proposal is very limited.
The same proposal was reviewed by the ISRP recently and given a Do Not Fund recommendation. Other than the addition of a summary of the investigator's qualifications and a paragraph suggesting which textbook the statistical test may be drawn from and that an unnamed statistical consultant would be sought (but giving no sampling designs, etc.), we see very little basis for changing the past assessment. This study won't answer the question it proposes to answer.
Recommendation:
Recommended Action
Date:
Oct 24, 2002
Comment:
Recommendation:
Do Not Fund
Date:
Nov 5, 2002
Comment:
Do Not Fund. We disagree with the CBFWA Recommended Action ranking. The same proposal was reviewed by the ISRP recently and given a Do Not Fund recommendation. Other than the addition of a summary of the investigator's qualifications and a paragraph suggesting which textbook the statistical test may be drawn from and that an unnamed statistical consultant would be sought (but giving no sampling designs, etc.), we see very little basis for changing the past assessment. With the information provided, the ISRP is not confident that the proposal will answer the question it proposes to answer. This proposal would investigate the possible exchange, between hatcheries and the environment, of two of the most serious bacterial diseases found in salmonid hatcheries of the Pacific Northwest, Bacterial Cold Water disease and Furunculosis, caused by Flavobacterium psychrophilum and Aeromonas salmonicida. Coho and steelhead are most susceptible but other salmonid species can be infected or act as carriers. These diseases are not limited to hatchery fish but also occur among wild populations. The proposed work could complement a similar proposal (#35039, USGS-CRRL) but the content of this proposal is very limited.
Recommendation:
Date:
Jan 21, 2003
Comment:
Statement of Potential Biological Benefit
Indirect biological benefits to listed stocks. Assessment using new technique to identify the distribution and potential disease vectors of two pathogens.
Comments
This project purports to address the continuing question of disease vectors into and out of hatchery and wild populations of salmonids. The study design and relevance to RPA 184 are both problematic.
Already ESA Required?
No
Biop?
No
Recommendation:
Do Not Fund (Tier 3)
Date:
Jun 11, 2003
Comment:
Category:
3. Other projects not recommended by staffComments: