FY 2003 Mainstem/Systemwide proposal 199009300
Contents
Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary
Reviews and Recommendations
Additional documents
Title | Type |
---|---|
199009300 Narrative | Narrative |
199009300 Powerpoint Presentation | Powerpoint Presentation |
199009300 Sponsor Response to the ISRP | Response |
Section 1. Administrative
Proposal title | Genetic Analysis of Oncorhynchus nerka (modified to include chinook salmon) |
Proposal ID | 199009300 |
Organization | University of Idaho (UI) |
Proposal contact person or principal investigator | |
Name | Madison S. Powell |
Mailing address | 3059F National Fish Hatchery Road Hagerman, ID 83332 |
Phone / email | 2088379096 / mpowell@uidaho.edu |
Manager authorizing this project | Madison S. Powell |
Review cycle | Mainstem/Systemwide |
Province / Subbasin | Mainstem/Systemwide / |
Short description | This ongoing project provides genetic information to assess immediate and long-term genetic risks to federally endangered Snake River sockeye and threatened Salmon River chinook salmon currently in artificial production programs. |
Target species | sockeye salmon (Endangered, Snake River ESU) and chinook salmon (Threatened, Snake River Spring Chinook ESU). |
Project location
Latitude | Longitude | Description |
---|---|---|
46.3267 | -116.8388 | University of Idaho, Moscow, Idaho |
42.8248 | -114.3787 | Hagerman Fish Culture Experiment Station, Hagerman, Idaho |
Reasonable and Prudent Alternatives (RPAs)
Sponsor-reported:
RPA |
---|
184 |
182 |
175 and 177 |
Relevant RPAs based on NMFS/BPA review:
Reviewing agency | Action # | BiOp Agency | Description |
---|---|---|---|
NMFS/BPA | Action 177 | NMFS | In 2002, BPA shall begin to implement and sustain NMFS-approved, safety-net projects. |
NMFS | Action 184 | NMFS | The Action Agencies and NMFS shall work within regional prioritization and congressional appropriation processes to establish and provide the appropriate level of FCRPS funding for a hatchery research, monitoring, and evaluation program consisting of studies to determine whether hatchery reforms reduce the risk of extinction for Columbia River basin salmonids and whether conservation hatcheries contribute to recovery. |
NMFS/BPA | Action 184 | NMFS | The Action Agencies and NMFS shall work within regional prioritization and congressional appropriation processes to establish and provide the appropriate level of FCRPS funding for a hatchery research, monitoring, and evaluation program consisting of studies to determine whether hatchery reforms reduce the risk of extinction for Columbia River basin salmonids and whether conservation hatcheries contribute to recovery. |
Section 2. Past accomplishments
Year | Accomplishment |
---|---|
2001 | Monthly genetic reports to Stanley Basin Technical Oversight Committee and Chinook Salmon Captive Propagation Technical Oversight Committee. "Phylogeography of Redfish Lake Sockeye Salmon" submitted for peer-review. |
2001 | Annual report for 2001 studies including parentage assignments and assignment tests for all returning Redfish Lake sockeye. Spawning matrices for Redfish Lake sockeye returns, assignment tests for safety-net chinook salmon, and their spawning matrices. |
2002 | Monthly genetic reports to Stanley Basin Technical Oversight Committee and Chinook Salmon Captive Propagation Technical Oversight Committee. |
2002 | Annual report for 2001 studies including parentage assignments and assignment tests for all returning Redfish Lake sockeye. Spawning matrices for Redfish Lake sockeye returns, assignment tests for safety-net chinook salmon, and their spawning matrices. |
Section 3. Relationships to other projects
Project ID | Title | Description |
---|---|---|
199700100 | Captive Rearing Project for Salmon River Chinook Salmon | This project provides genetic analysis of threatened River chinook salmon. Program 199700100 provides tissue samples for analysis. |
199107200 | Redfish Lake Sockeye Salmon Captive Broodstock Program | This project provides genetic analysis of endangered Redfish Lake sockeye. Program 199107200 provides tissue samples for analysis. |
199204000 | Redfish Lake Sockeye Salmon Captive Broodstock Rearing and Research | This project provides genetic analysis of endangered Redfish Lake sockeye. Program 199204000 provides tissue samples for analysis. |
199606700 | Manchester Spring Chinook Broodstock | This project provides genetic analysis of threatened River chinook salmon. Program 199606700 provides tissue samples for analysis. |
Section 4. Budget for Planning and Design phase
Task-based budget
Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
---|
Outyear objectives-based budget
Objective | Starting FY | Ending FY | Estimated cost |
---|
Outyear budgets for Planning and Design phase
Section 5. Budget for Construction and Implementation phase
Task-based budget
Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
---|---|---|---|---|
1. Perform ongoing microsatellite analyses on tissue samples from sockeye outmigrant assemblages and returns. | Task a. Assign out migrant assemblages to population origin and captive parental crosses and estimate effective population sizes. | ongoing | $21,420 | |
1 | Task b. Compare genetic similarity of outmigrants and broodstock from the same and different year classes. | ongoing | $21,421 | |
1 | Task c. Examine genetic similarity of production vs. conservation subunits within the captive broodstock program. | ongoing | $14,281 | |
2. Screen tissue samples from Redfish Lake creel surveys and commercial harvest. | Task a. Screen creel samples for incidental take of listed sockeye in kokanee harvest. | ongoing | $10,203 | |
2 | Task b. Screen stray O. nerka within Columbia Basin for Redfish Lake origin. | ongoing | $2,400 | |
3. Continue genetic analysis of captively reared Salmon River chinook salmon and evaluation of effective population size and genetic diversity using microsatellite analyses. | Task a. Assign parental crosses and examine genetic similarity of captively reared Chinook to construct breeding matrices for MAI. | ongoing | $20,081 | |
3 | Task b. Compare genetic changes between hatchery origin outmigrants and returns vs. wild origin fish in the same location. | ongoing | $26,430 | |
3 | Task c. Evaluate effective population size estimates with other Chinook populations. | ongoing | $10,200 |
Outyear objectives-based budget
Objective | Starting FY | Ending FY | Estimated cost |
---|---|---|---|
1. Perform ongoing microsatellite analyses on tissue samples from sockeye outmigrant assemblages and returns. | 2003 | 2008 | $342,732 |
2. Screen tissue samples from Redfish Lake creel surveys and commercial harvest. | 2003 | 2008 | $75,618 |
3. Continue genetic analysis of captively reared Salmon River chinook salmon and evaluation of effective population size and genetic diversity using microsatellite analyses. | 2003 | 2008 | $170,133 |
Outyear budgets for Construction and Implementation phase
FY 2004 | FY 2005 | FY 2006 | FY 2007 |
---|---|---|---|
$98,080 | $98,080 | $98,080 | $98,080 |
Section 6. Budget for Operations and Maintenance phase
Task-based budget
Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
---|
Outyear objectives-based budget
Objective | Starting FY | Ending FY | Estimated cost |
---|
Outyear budgets for Operations and Maintenance phase
Section 7. Budget for Monitoring and Evaluation phase
Task-based budget
Objective | Task | Duration in FYs | Estimated 2003 cost | Subcontractor |
---|
Outyear objectives-based budget
Objective | Starting FY | Ending FY | Estimated cost |
---|
Outyear budgets for Monitoring and Evaluation phase
Section 8. Estimated budget summary
Itemized budget
Item | Note | FY 2003 cost |
---|---|---|
Personnel | FTE: M. Powell (2 mo.) Research Support Scientist (12 mo.) Master's Student (6 mo.) Lab Tech. (6 mo.) | $58,742 |
Fringe | from 0.01 to 0.36 | $16,507 |
Supplies | Tips, tubes, chemicals, enzymes, cappillaries etc. | $17,700 |
Travel | 1 professional meeting, 12 monthly TOC meetings | $3,200 |
Indirect | Ag experiment ind. cost rate = 31.5% | $30,287 |
Capital | none | $0 |
NEPA | none | $0 |
PIT tags | # of tags: none | $0 |
Subcontractor | none | $0 |
Other | none | $0 |
$126,436 |
Total estimated budget
Total FY 2003 cost | $126,436 |
Amount anticipated from previously committed BPA funds | $0 |
Total FY 2003 budget request | $126,436 |
FY 2003 forecast from 2002 | $65,000 |
% change from forecast | 94.5% |
Reason for change in estimated budget
Microsatellite analyses have been expanded to include pedigree analysis and parentage assignment of all returning adults (sockeye and chinook). The "increase" is a 3.2% reduction from FY2002. The program has not scaled down as anticipated because of good returns and large numbers of outmigrant samples and others. It is anticipated that this program will have a complete dataset of three sockeye generations and two chinook generations by 2008 (expected termination date) for comparison and genetic evaluation of the captive programs.
Reason for change in scope
There has been no change in scope.
Cost sharing
Organization | Item or service provided | Amount | Cash or in-kind |
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Reviews and recommendations
This information was not provided on the original proposals, but was generated during the review process.
Fundable only if response is adequate
Aug 2, 2002
Comment:
A response is required. This is clearly high priority work that warrants continued funding and is overall a strong proposal. In general, the ISRP's FY00 comments remain applicable. The proposal is well written and addresses the genetic variation in Columbia River sockeye salmon, particularly in the listed stock (Redfish Lake) and its captive breeding program, plus the impact of captive rearing on three listed chinook salmon populations. These populations are severely depressed and require careful genetic monitoring to maintain the remaining genetic variation.The ISRP's primary concern was why results of past monitoring were not presented? There are some obvious issues of concern such as what has been variation of family size in families of sockeye? Or, has mortality in the captive brood programs (sockeye or Chinook) been random among families? How has data been used to structure mating schemes? In the absence of any presentation of these data, the reviewers cannot comment on the timeliness of these analyses or adequacy of analyses.
The proposal provides additional information on reports and publications that have resulted from these studies to address some of the ISRP's FY00 comments. One hopes that with the long-term dataset that is being generated by this study that additional peer-reviewed publications will arise from the work. The proposal has a long-term monitoring component that is needed to provide consistency and insights into the Redfish Lake sockeye captive broodstock effort. This effort, while necessary, is largely routine by this time.
The most interesting aspect of the proposal, which could have been more fully described, is the more recent use of microsatellite loci analyses to develop pedigrees, identify parentage, and to set up MAI (Maximal Avoidance of Inbreeding) matrices to guide captive breeding options for severely depressed chinook populations in the East Fork of the Salmon, West Fork of the Yankee Fork, and so on. This approach has very strong applied conservation biology implications and deserves to be better described in the proposal with respect to its methods, application, and management implications.
This project also monitors the bi-catch of sockeye salmon in a sport fishery for kokanee in Redfish Lake and has demonstrated the bi-catch of anadromous or residual sockeye. While this concern is not the responsibility of this author, it is a concern that in a lake with a listed sockeye salmon stock, at an extremely depressed population size, that a kokanee fishery would be allowed at all. What impact is allowed on sockeye in this fishery and how is it justified?
Comment:
The FY 2004 budget may increase to FY 2003 level.Comment:
Our current proposed budget ($126,436.00) represents an 8.4% decrease from our FY2002 budget ($138,015.00). We have removed of all cost-of-living increases for project personnel and all capital acquisitions from the FY 2003 budget. The remaining budget includes only objectives and personnel essential to carry out stated scientific goals. Our level of cost sharing from the University is currently at a maximum with respect to this project. We anticipate one additional year (FY2004) at the FY2002 budget level in order to allow us to complete genetic analysis of captive-reared chinook populations within the Columbia Basin. Following FY2004, our budget requirements will substantially decrease (22.2%) for FY2005 and remain at this level until FY2008, the expected completion date of the sockeye genetic analysis objectives.Comment:
Fundable. We agree with the CBFWA review and Urgent ranking. This is clearly high priority work that warrants continued funding. The proposal is well written. The project addresses the genetic variation in Columbia River sockeye salmon, particularly in the listed stock (Redfish Lake) and its captive breeding program, plus the impact of captive rearing on three listed chinook salmon populations. These populations are severely depressed and require careful genetic monitoring to maintain the remaining genetic variation. This project also monitors the bycatch of sockeye salmon in a sport fishery for kokanee in Redfish Lake and has demonstrated the bycatch of anadromous or residual sockeye. While this concern is not the responsibility of this project, it is a concern that in a lake with a listed sockeye salmon stock, at an extremely depressed population size, that a kokanee fishery would be allowed at all. The ISRP remains concerned about what impact is allowed on sockeye in this fishery and how is it justified?The proposal has a long term monitoring component that is needed to provide consistency and insights into the Redfish Lake sockeye captive broodstock effort. The proposal also provides additional information on reports and publications that have resulted from this study (and related ones) to address some of the ISRP's FY00 review comments. One hopes that with the long-term dataset that is being generated by this study that additional peer-reviewed publications will arise from the work. The project sponsors provided a thorough response that adequately addressed the ISRP's preliminary review questions, including additional description and details on the recent use of microsatellite loci analyses to develop pedigrees, identify parentage, and to set up MAI (Maximal Avoidance of Inbreeding) matrices to guide captive breeding options for severely depressed chinook populations in the East Fork of the Salmon and West Fork of the Yankee Fork.
Comment:
Statement of Potential Biological BenefitIndirect. This project provides genetic profiles for returning sockeye and chinook salmon currently in captive rearing or captive broodstock programs. These genetic profiles can be used to assess immediate and long-term genetic risks to Snake River sockeye and Salmon River chinook salmon from captive propagation programs
Comments
This proposal is part of an ongoing project to provide genetic information for assessment of immediate and long-term genetic risks to ESA-listed Snake River sockeye and Salmon River chinook salmon currently in captive rearing programs. As such, it will provide a biological benefit by providing genetic monitoring necessary to maintain genetic variation. Of scientific interest to us is the use of microsatellite DNA analyses to develop pedigrees, identify parentage, and to define Maximal Avoidance of Inbreeding matrices to guide captive breeding options for the chinook salmon populations in the East Fork of the Salmon, West Fork of the Yankee Fork, etc. This approach may assist conservation biology.
Already ESA Required?
No
Biop?
Yes
Comment:
Category:1. Council Staff preferred projects that fit province allocation
Comments:
Link to programmatic captive broodstock issue.
Comment:
Review for scope and budget for chinook. Budget consistent with NPCC recommendation.Comment:
NW Power and Conservation Council's FY 2006 Project Funding Review
expense
May 2005
FY05 NPCC start of year: | FY06 NPCC staff preliminary: | FY06 NPCC July draft start of year: |
$126,000 | $98,000 | $98,000 |
Sponsor comments: See comment at Council's website