FY 2003 Mainstem/Systemwide proposal 199009300

Additional documents

TitleType
199009300 Narrative Narrative
199009300 Powerpoint Presentation Powerpoint Presentation
199009300 Sponsor Response to the ISRP Response

Section 1. Administrative

Proposal titleGenetic Analysis of Oncorhynchus nerka (modified to include chinook salmon)
Proposal ID199009300
OrganizationUniversity of Idaho (UI)
Proposal contact person or principal investigator
NameMadison S. Powell
Mailing address3059F National Fish Hatchery Road Hagerman, ID 83332
Phone / email2088379096 / mpowell@uidaho.edu
Manager authorizing this projectMadison S. Powell
Review cycleMainstem/Systemwide
Province / SubbasinMainstem/Systemwide /
Short descriptionThis ongoing project provides genetic information to assess immediate and long-term genetic risks to federally endangered Snake River sockeye and threatened Salmon River chinook salmon currently in artificial production programs.
Target speciessockeye salmon (Endangered, Snake River ESU) and chinook salmon (Threatened, Snake River Spring Chinook ESU).
Project location
LatitudeLongitudeDescription
46.3267 -116.8388 University of Idaho, Moscow, Idaho
42.8248 -114.3787 Hagerman Fish Culture Experiment Station, Hagerman, Idaho
Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA
184
182
175 and 177

Relevant RPAs based on NMFS/BPA review:

Reviewing agencyAction #BiOp AgencyDescription
NMFS/BPA Action 177 NMFS In 2002, BPA shall begin to implement and sustain NMFS-approved, safety-net projects.
NMFS Action 184 NMFS The Action Agencies and NMFS shall work within regional prioritization and congressional appropriation processes to establish and provide the appropriate level of FCRPS funding for a hatchery research, monitoring, and evaluation program consisting of studies to determine whether hatchery reforms reduce the risk of extinction for Columbia River basin salmonids and whether conservation hatcheries contribute to recovery.
NMFS/BPA Action 184 NMFS The Action Agencies and NMFS shall work within regional prioritization and congressional appropriation processes to establish and provide the appropriate level of FCRPS funding for a hatchery research, monitoring, and evaluation program consisting of studies to determine whether hatchery reforms reduce the risk of extinction for Columbia River basin salmonids and whether conservation hatcheries contribute to recovery.

Section 2. Past accomplishments

YearAccomplishment
2001 Monthly genetic reports to Stanley Basin Technical Oversight Committee and Chinook Salmon Captive Propagation Technical Oversight Committee. "Phylogeography of Redfish Lake Sockeye Salmon" submitted for peer-review.
2001 Annual report for 2001 studies including parentage assignments and assignment tests for all returning Redfish Lake sockeye. Spawning matrices for Redfish Lake sockeye returns, assignment tests for safety-net chinook salmon, and their spawning matrices.
2002 Monthly genetic reports to Stanley Basin Technical Oversight Committee and Chinook Salmon Captive Propagation Technical Oversight Committee.
2002 Annual report for 2001 studies including parentage assignments and assignment tests for all returning Redfish Lake sockeye. Spawning matrices for Redfish Lake sockeye returns, assignment tests for safety-net chinook salmon, and their spawning matrices.

Section 3. Relationships to other projects

Project IDTitleDescription
199700100 Captive Rearing Project for Salmon River Chinook Salmon This project provides genetic analysis of threatened River chinook salmon. Program 199700100 provides tissue samples for analysis.
199107200 Redfish Lake Sockeye Salmon Captive Broodstock Program This project provides genetic analysis of endangered Redfish Lake sockeye. Program 199107200 provides tissue samples for analysis.
199204000 Redfish Lake Sockeye Salmon Captive Broodstock Rearing and Research This project provides genetic analysis of endangered Redfish Lake sockeye. Program 199204000 provides tissue samples for analysis.
199606700 Manchester Spring Chinook Broodstock This project provides genetic analysis of threatened River chinook salmon. Program 199606700 provides tissue samples for analysis.

Section 4. Budget for Planning and Design phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
1. Perform ongoing microsatellite analyses on tissue samples from sockeye outmigrant assemblages and returns. Task a. Assign out migrant assemblages to population origin and captive parental crosses and estimate effective population sizes. ongoing $21,420
1 Task b. Compare genetic similarity of outmigrants and broodstock from the same and different year classes. ongoing $21,421
1 Task c. Examine genetic similarity of production vs. conservation subunits within the captive broodstock program. ongoing $14,281
2. Screen tissue samples from Redfish Lake creel surveys and commercial harvest. Task a. Screen creel samples for incidental take of listed sockeye in kokanee harvest. ongoing $10,203
2 Task b. Screen stray O. nerka within Columbia Basin for Redfish Lake origin. ongoing $2,400
3. Continue genetic analysis of captively reared Salmon River chinook salmon and evaluation of effective population size and genetic diversity using microsatellite analyses. Task a. Assign parental crosses and examine genetic similarity of captively reared Chinook to construct breeding matrices for MAI. ongoing $20,081
3 Task b. Compare genetic changes between hatchery origin outmigrants and returns vs. wild origin fish in the same location. ongoing $26,430
3 Task c. Evaluate effective population size estimates with other Chinook populations. ongoing $10,200
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
1. Perform ongoing microsatellite analyses on tissue samples from sockeye outmigrant assemblages and returns. 2003 2008 $342,732
2. Screen tissue samples from Redfish Lake creel surveys and commercial harvest. 2003 2008 $75,618
3. Continue genetic analysis of captively reared Salmon River chinook salmon and evaluation of effective population size and genetic diversity using microsatellite analyses. 2003 2008 $170,133
Outyear budgets for Construction and Implementation phase
FY 2004FY 2005FY 2006FY 2007
$98,080$98,080$98,080$98,080

Section 6. Budget for Operations and Maintenance phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2003 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget
ItemNoteFY 2003 cost
Personnel FTE: M. Powell (2 mo.) Research Support Scientist (12 mo.) Master's Student (6 mo.) Lab Tech. (6 mo.) $58,742
Fringe from 0.01 to 0.36 $16,507
Supplies Tips, tubes, chemicals, enzymes, cappillaries etc. $17,700
Travel 1 professional meeting, 12 monthly TOC meetings $3,200
Indirect Ag experiment ind. cost rate = 31.5% $30,287
Capital none $0
NEPA none $0
PIT tags # of tags: none $0
Subcontractor none $0
Other none $0
$126,436
Total estimated budget
Total FY 2003 cost$126,436
Amount anticipated from previously committed BPA funds$0
Total FY 2003 budget request$126,436
FY 2003 forecast from 2002$65,000
% change from forecast94.5%
Reason for change in estimated budget

Microsatellite analyses have been expanded to include pedigree analysis and parentage assignment of all returning adults (sockeye and chinook). The "increase" is a 3.2% reduction from FY2002. The program has not scaled down as anticipated because of good returns and large numbers of outmigrant samples and others. It is anticipated that this program will have a complete dataset of three sockeye generations and two chinook generations by 2008 (expected termination date) for comparison and genetic evaluation of the captive programs.

Reason for change in scope

There has been no change in scope.

Cost sharing
OrganizationItem or service providedAmountCash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

Recommendation:
Fundable only if response is adequate
Date:
Aug 2, 2002

Comment:

A response is required. This is clearly high priority work that warrants continued funding and is overall a strong proposal. In general, the ISRP's FY00 comments remain applicable. The proposal is well written and addresses the genetic variation in Columbia River sockeye salmon, particularly in the listed stock (Redfish Lake) and its captive breeding program, plus the impact of captive rearing on three listed chinook salmon populations. These populations are severely depressed and require careful genetic monitoring to maintain the remaining genetic variation.

The ISRP's primary concern was why results of past monitoring were not presented? There are some obvious issues of concern such as what has been variation of family size in families of sockeye? Or, has mortality in the captive brood programs (sockeye or Chinook) been random among families? How has data been used to structure mating schemes? In the absence of any presentation of these data, the reviewers cannot comment on the timeliness of these analyses or adequacy of analyses.

The proposal provides additional information on reports and publications that have resulted from these studies to address some of the ISRP's FY00 comments. One hopes that with the long-term dataset that is being generated by this study that additional peer-reviewed publications will arise from the work. The proposal has a long-term monitoring component that is needed to provide consistency and insights into the Redfish Lake sockeye captive broodstock effort. This effort, while necessary, is largely routine by this time.

The most interesting aspect of the proposal, which could have been more fully described, is the more recent use of microsatellite loci analyses to develop pedigrees, identify parentage, and to set up MAI (Maximal Avoidance of Inbreeding) matrices to guide captive breeding options for severely depressed chinook populations in the East Fork of the Salmon, West Fork of the Yankee Fork, and so on. This approach has very strong applied conservation biology implications and deserves to be better described in the proposal with respect to its methods, application, and management implications.

This project also monitors the bi-catch of sockeye salmon in a sport fishery for kokanee in Redfish Lake and has demonstrated the bi-catch of anadromous or residual sockeye. While this concern is not the responsibility of this author, it is a concern that in a lake with a listed sockeye salmon stock, at an extremely depressed population size, that a kokanee fishery would be allowed at all. What impact is allowed on sockeye in this fishery and how is it justified?


Recommendation:
Urgent
Date:
Oct 24, 2002

Comment:

The FY 2004 budget may increase to FY 2003 level.
Recommendation:
Urgent
Date:
Oct 24, 2002

Comment:

Our current proposed budget ($126,436.00) represents an 8.4% decrease from our FY2002 budget ($138,015.00). We have removed of all cost-of-living increases for project personnel and all capital acquisitions from the FY 2003 budget. The remaining budget includes only objectives and personnel essential to carry out stated scientific goals. Our level of cost sharing from the University is currently at a maximum with respect to this project. We anticipate one additional year (FY2004) at the FY2002 budget level in order to allow us to complete genetic analysis of captive-reared chinook populations within the Columbia Basin. Following FY2004, our budget requirements will substantially decrease (22.2%) for FY2005 and remain at this level until FY2008, the expected completion date of the sockeye genetic analysis objectives.
Recommendation:
Fund
Date:
Nov 5, 2002

Comment:

Fundable. We agree with the CBFWA review and Urgent ranking. This is clearly high priority work that warrants continued funding. The proposal is well written. The project addresses the genetic variation in Columbia River sockeye salmon, particularly in the listed stock (Redfish Lake) and its captive breeding program, plus the impact of captive rearing on three listed chinook salmon populations. These populations are severely depressed and require careful genetic monitoring to maintain the remaining genetic variation. This project also monitors the bycatch of sockeye salmon in a sport fishery for kokanee in Redfish Lake and has demonstrated the bycatch of anadromous or residual sockeye. While this concern is not the responsibility of this project, it is a concern that in a lake with a listed sockeye salmon stock, at an extremely depressed population size, that a kokanee fishery would be allowed at all. The ISRP remains concerned about what impact is allowed on sockeye in this fishery and how is it justified?

The proposal has a long term monitoring component that is needed to provide consistency and insights into the Redfish Lake sockeye captive broodstock effort. The proposal also provides additional information on reports and publications that have resulted from this study (and related ones) to address some of the ISRP's FY00 review comments. One hopes that with the long-term dataset that is being generated by this study that additional peer-reviewed publications will arise from the work. The project sponsors provided a thorough response that adequately addressed the ISRP's preliminary review questions, including additional description and details on the recent use of microsatellite loci analyses to develop pedigrees, identify parentage, and to set up MAI (Maximal Avoidance of Inbreeding) matrices to guide captive breeding options for severely depressed chinook populations in the East Fork of the Salmon and West Fork of the Yankee Fork.


Recommendation:
Date:
Jan 21, 2003

Comment:

Statement of Potential Biological Benefit
Indirect. This project provides genetic profiles for returning sockeye and chinook salmon currently in captive rearing or captive broodstock programs. These genetic profiles can be used to assess immediate and long-term genetic risks to Snake River sockeye and Salmon River chinook salmon from captive propagation programs

Comments
This proposal is part of an ongoing project to provide genetic information for assessment of immediate and long-term genetic risks to ESA-listed Snake River sockeye and Salmon River chinook salmon currently in captive rearing programs. As such, it will provide a biological benefit by providing genetic monitoring necessary to maintain genetic variation. Of scientific interest to us is the use of microsatellite DNA analyses to develop pedigrees, identify parentage, and to define Maximal Avoidance of Inbreeding matrices to guide captive breeding options for the chinook salmon populations in the East Fork of the Salmon, West Fork of the Yankee Fork, etc. This approach may assist conservation biology.

Already ESA Required?
No

Biop?
Yes


Recommendation:
Fund (Tier 1)
Date:
Jun 11, 2003

Comment:

Category:
1. Council Staff preferred projects that fit province allocation

Comments:
Link to programmatic captive broodstock issue.


Recommendation:
Date:
Aug 4, 2003

Comment:

Review for scope and budget for chinook. Budget consistent with NPCC recommendation.
Recommendation:
Date:
Sep 20, 2003

Comment:


REVIEW:
NW Power and Conservation Council's FY 2006 Project Funding Review
Funding category:
expense
Date:
May 2005
FY05 NPCC start of year:FY06 NPCC staff preliminary:FY06 NPCC July draft start of year:
$126,000 $98,000 $98,000

Sponsor comments: See comment at Council's website