FY 2003 Mainstem/Systemwide proposal 200002900

Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary

Reviews and Recommendations

Additional documents

Title	Type
200002900 Narrative	Narrative
200002900 Powerpoint Presentation	Powerpoint Presentation
200002900 Sponsor Response to the ISRP	Response

Section 1. Administrative

Proposal title	Identification and thermal requirements of larval Pacific, river, and western brook lampreys
Proposal ID	200002900
Organization	U.S. Geological Survey (USGS)
Proposal contact person or principal investigator
Name	Jennifer M. Bayer
Mailing address	CRRL, 5501A Cook-Underwood Road Cook, WA 98605
Phone / email	5095382299 / jennifer_bayer@usgs.gov
Manager authorizing this project	James G. Seelye
Review cycle	Mainstem/Systemwide
Province / Subbasin	Mainstem/Systemwide /
Short description	Determine morphological and molecular characteristics that differentiate sympatric larval lampreys and evaluate thermal tolerances of larval lampreys by species
Target species	Pacific lamprey (Lampetra tridentata); river lamprey (L. ayresi); western brook lamprey (L. richardsoni)

Project location

Latitude	Longitude	Description
		systemwide

Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agency	Action #	BiOp Agency	Description

Section 2. Past accomplishments

Year	Accomplishment
2000	Annual progress report to Bonneville Power Administration
2001	Annual progress report to Bonneville Power Administration
2001	Presentation to the Native American Fish and Wildlife Society
2002	Presentation to the International Congress on the Biology of Fishes, Vancouver, B.C. July 2002

Section 3. Relationships to other projects

Project ID	Title	Description
199402600	Pacific Lamprey Research and Restoration	Proposed work will complement Project 199402600 by providing tools to use in the implementation and evaluation of restoration of lamprey populations in the Umatilla River.
200002800	Evaluate Status of Pacific Lamprey in the Clearwater River Drainage, Idaho	Proposed work will complement Project 200002800 by providing tools to identify larval lampreys and information to guide the design of lamprey distribution surveys.
25007	Determine lamprey species composition, larval distribution and adult abundance in the Deschutes Subbasin	Proposed work will complement Project 25007 by providing tools to identify larval lampreys and information to guide the design of lamprey distribution surveys.
200001400	Evaluate habitat use and population dynamics of lampreys in Cedar Creek	Proposed work will complement Project 200001400 by providing tools to identify larval lampreys and information to guide the design of lamprey distribution surveys.
	Evaluate Status of Pacific Lamprey in the Willamette River Subbasin	Sponsors of this work are willing to cooperate with USGS by providing specimens.. Also, USGS work will complement this proposed work by providing tools to identify larval lampreys and information to guide the design of lamprey distribution surveys.

Section 4. Budget for Planning and Design phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2003 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2003 cost
1. Determine diagnostic characteristics of egg and larval stages of lampreys	Spawn adult Pacific, river, and western brook lamprey in the laboratory, collect a time series of resulting progeny, and conduct morphometric analysis to determine diagnostic characteristics that differentiate these species	4	$43,925
2. Evaluate temperature effects on the survival and early development of lampreys	Rear early life history stages of each species at four temperatures, document survival and timing of developmental events	4	$43,925
3. Develop molecular techniques suitable for distinguishing Columbia River Basin lamprey species.	a. Determine appropriate molecular techniques for identifying Columbia River Basin lampreys to the species level; b. validate these molecular techniques as appropriate for identification of lampreys distributed throughout the Columbia River Basin	2	$99,095

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost
3. Develop molecular techniques suitable for distinguishing Columbia River Basin lamprey species.	2003	2004	$175,000

Outyear budgets for Construction and Implementation phase

FY 2004
$75,000

Section 6. Budget for Operations and Maintenance phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2003 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2003 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget

Item	Note	FY 2003 cost
Personnel	FTE: 2.4	$90,377
Fringe	.3	$25,657
Supplies		$14,000
Travel		$10,000
Indirect	annually determined rate	$46,911
		$186,945

Total estimated budget

Total FY 2003 cost	$186,945
Amount anticipated from previously committed BPA funds	$0
Total FY 2003 budget request	$186,945
FY 2003 forecast from 2002	$0
% change from forecast	0.0%

Cost sharing

Organization	Item or service provided	Amount	Cash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

REVIEW:
ISRP Preliminary Review (ISRP 2002-13)

Recommendation:
Fundable only if response is adequate

Date:
Aug 2, 2002

Comment:

A response is needed. This is a project, now (2002) in its third year, to provide basic biological data on the species of lampreys occurring together in the Columbia River basin. With anadromous Pacific lamprey populations in decline, likely because of hydrosystem effects, and mitigation measures underway, there is a strong need to be able to identify that species from other lamprey species (western brook lamprey and river lamprey) that also reside in streams during early life stages. The project has focused initially on basic morphological tools of the taxonomist for differentiating eggs and early larvae raised in the laboratory, but there are plans to expand the effort to use biochemical genetic markers of species identity. Additionally, the rearing of larvae in the laboratory has been carried out at four temperatures in order to characterize the temperature requirements for survival of these stages, which might differ. An equipment failure caused delay in some aspects of the intended schedule, so the study team proposes another two years for completion. The final years will emphasize replicated work on morphological and temperature-effect studies, preparation of manuscripts on that work, and more emphasis on the genetic differentiation techniques.

The proposal was well prepared and informative, and met the ISRP review requirements (monitoring and evaluation was not considered especially relevant). The project has yielded good results for the first years of the study that were well presented in the proposal. There is an adequately prepared rationale and justification based on the Council's Fish and Wildlife Program and the uncertainties, goals, and objectives from the Columbia River Lamprey Program Summary and the Mainstem/Systemwide solicitation (which included the relevant Biological Opinion information). The proponents demonstrated knowledge of related projects in the basin, and coordinate with them informally. There are no monitoring and evaluation aspects to the planned studies. The staff seems well qualified to do the work.

Because this is a 3-year solicitation, it was surprising that the proponents did not extend the proposal beyond the next two years. It is laudable to see a planned termination, but this may have been an oversight. The ISRP suspects there are more basic biological data needed in the early life stage development of the three species. There are likely limiting habitat factors other than temperature. Because the project's monitoring and evaluation component (essentially none) is the weakest of the ISRP evaluation criteria, field sampling to use and test the identification techniques might have been proposed. The ISRP requests a response to address such follow-up research for the third year of the solicitation cycle (or reaffirmation that only two years of funding was intended).

This project was not selected for review by the Action Agency/NMFS RME Work Group.

REVIEW:
CBFWA Funding Recommendation

Recommendation:
Urgent

Date:
Oct 24, 2002

Comment:

Fund for two years as proposed to complete project work and protect prior investment in project. This information will be useful to managers as lamprey management plans are developed. An element has been added to the identification task within the proposal. A molecular tool is being investigated to assist with morphometric identification. This new task should be retained during funding.

REVIEW:
CBFWA FY03 Budget Review Comments

Recommendation:
Urgent

Date:
Oct 24, 2002

Comment:

The proposal for this project describes three objectives: Objective 1) determine diagnostic characteristics of egg and larval stages of lampreys; Objective 2) evaluate temperature effects on the survival and early development of lampreys; and Objective 3) develop molecular techniques suitable for distinguishing Columbia River Basin lamprey species. The funding request for the first two of these objectives is necessary to provide a final completion product for this work as originally proposed. The funding level requested is a bare minimum already (with USGS actually picking up a greater portion of staff costs than expressed in the cost-share estimate in the proposal) and no level of reduction is possible to complete the tasks as described. Objective 3 is really an additional element of Objective 2. We described these as separate objectives in the proposal so that it would be transparent to reviewers why we needed additional funds and time. We feel this element is critical to reaching our goal of providing lamprey identification methodology to managers in the Columbia River Basin. However, since it is possible to defer this element of the work to a later year, I have placed the funding request for FY03 ($99,095) and FY04 ($75,000) for this element in the table below as you requested. We feel strongly that it would be more efficient to perform these activities simultaneously.

REVIEW:
ISRP Final Review (ISRP 2002-14)

Recommendation:
Fund

Date:
Nov 5, 2002

Comment:

Fundable to complete in two years as proposed. The response was adequate, but minimal. We agree with the CBFWA review and "urgent" ranking.

This project, now (2002) in its third year, will provide basic biological data on the species of lampreys occurring together in the Columbia River basin. With anadromous Pacific lamprey populations in decline, likely because of hydrosystem effects, and mitigation measures underway, there is a strong need to be able to identify that species from other lamprey species (western brook lamprey and river lamprey) that also reside in streams during early life stages. The project has focused initially on basic morphological tools of the taxonomist for differentiating eggs and early larvae raised in the laboratory, but there are plans to expand the effort to use biochemical genetic markers of species identity. Additionally, the rearing of larvae in the laboratory has been carried out at four temperatures in order to characterize the temperature requirements for survival of these stages, which might differ. An equipment failure caused delay in some aspects of the intended schedule, so the study team proposes another two years for completion. The final years will emphasize replicated work on morphological and temperature-effect studies, preparation of manuscripts on that work, and more emphasis on the genetic differentiation techniques.

REVIEW:
NMFS Review

Recommendation:

Date:
Jan 21, 2003

Comment:

Statement of Potential Biological Benefit

Comments
Not Reviewed

Already ESA Required?

Biop?
No

REVIEW:
BPA Funding Recommendation

Recommendation:
Fund

Date:
Jun 2, 2003

Comment:

BPA Phase 3

REVIEW:
NWPPC Funding Recommendation

Recommendation:
Fund (Tier 1)

Date:
Jun 11, 2003

Comment:

Category:
1. Council Staff preferred projects that fit province allocation

Comments:
Relatively small dollar figure for two year ongoing.

REVIEW:
Project Sponsor Request for FY04-05

Recommendation:

Date:
Sep 20, 2003

Comment:

REVIEW:
BPA Funding Decision

Recommendation:
Do Not fund

Date:
Oct 2, 2003

Comment:

Placeholder funds to enable project closeout during the first quarter of FY 2004.