FY 1999 proposal 9084

Additional documents

TitleType
9084 Narrative Narrative

Section 1. Administrative

Proposal titleAssessing Genetic Variation Among Columbia Basin White Sturgeon Populations
Proposal ID9084
OrganizationUniversity of Idaho (UI)
Proposal contact person or principal investigator
NameMadison S. Powell
Mailing address3059 F National Fish Hatchery Road Hagerman, ID 83332
Phone / email2088379096 / fishdna@micron.net
Manager authorizing this project
Review cycleFY 1999
Province / SubbasinSystemwide / Systemwide
Short descriptionGenetic variation and stock structure among white sturgeon populations in the Columbia Basin based on analysis of mitochondrial and nuclear DNA.
Target species
Project location
LatitudeLongitudeDescription
Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agencyAction #BiOp AgencyDescription

Section 2. Past accomplishments

YearAccomplishment

Section 3. Relationships to other projects

Project IDTitleDescription
White sturgeon mitigation and restoration in the Columbia and Snake Rivers This project will provide genetic information for management. The 86-50 project provides tissue samples for this project.
Kootenai River ecosystem and fisheries improvement study This project will provide genetic information on an endangered population. The 94-49 project provides tissue samples for this project.
Kootenai River fisheries studies This project will provide genetic information on an endangered population. The 88-64 project provides tissue samples for this project.
Kootenai river fisheries investigations This project will provide genetic information on an endangered population. The 88-65 project provides tissue samples for this project.

Section 4. Budget for Planning and Design phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 1999 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 1999 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Construction and Implementation phase

Section 6. Budget for Operations and Maintenance phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 1999 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 1999 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget
ItemNoteFY 1999 cost
Personnel M. Powell, 6 mo. @ 24.00/hr. (1040 h) P. Anders, 12 mo. @ 16.90/hr. (2080 h) $60,112
Fringe 28.5% for both $17,132
Supplies chemicals, pipet tips, tubes, gloves etc. $20,000
Operating Equipment service and calibration, UPS shipping, Federal Express, long distance calls/faxing $2,000
Capital 1 Power Macintosh Computer and Perkin Elmer ABI Genotyper Software $11,000
Travel 1 professional meeting (AFS), 2 people, travel between laboratories $1,500
Indirect off campus indirect cost @ 25.8% $25,992
Subcontractor $0
$137,736
Total estimated budget
Total FY 1999 cost$137,736
Amount anticipated from previously committed BPA funds$0
Total FY 1999 budget request$137,736
FY 1999 forecast from 1998$0
% change from forecast0.ToString("0.0%"))
Cost sharing
OrganizationItem or service providedAmountCash or in-kind
Outyear budget totals

(working on it)

Other budget explanation

Schedule Constraints: Difficult sequencing or equipment breakdown for automated sequencing may lengthen the time required to complete Objective #2 and some of Objective #3. Projections for completion are: Objective #1; 1999, Objective #2; 2000, Objective #3; 2001


Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

Recommendation:
Fundable
Date:
May 13, 1998

Comment:

Presentation: The goal is to provide information to develop and implement future management actions for sturgeon.

Sturgeon life history used to allow gene flow. Hydro development has restricted the gene flow and the reproductive genes have been compromised. What do we conserve? Where do we conserve? What is population? Is the Columbia Basin one gene pool? The objective of the study is to compare inter- and intra population variation by looking at mitochondrial sequencing and nuclear genetic variation. This is the first comprehensive study. We have received over 60 samples from a variety of locations.

Questions/Answers:

Task 1b in project 8605000 seems to address this for $40,000, your budget is $100,000. Response: The $40,000 in 8605000 is for lab work. If this project (9084) is funded 8605000 won't use the $40,000. The scope of this project is bigger geographically and scientifically.

All four sturgeon projects have a genetic component. It seems like we are paying for this twice. Response: All of the sponsors recognized the need and do not intend to duplicate each other. This is a comprehensive project.

How much money goes into genetics? BPA could open their own lab cheaper. Is the technology to the point that if we do this study will it resolve the question or will we need more work? Answer: Yes. The life histories for salmon are opposite from sturgeon. Sturgeon are more simple than salmon. Getting baseline information before the opportunity is lost will lead to a more controlled approach to sturgeon. This information can identify issues related to transferring and stocking.

Is there cost sharing? Answer: National Science Foundation money (since 1987) runs out this summer and probably won't be renewed.

In Objective 2, are 10 individuals considered random samples? Answer: They would be. We want minimum 60 samples from each location. And a 95% statistical confidence for 5% of the individuals. This is the last key piece of information needed to move toward restoration and supplementation.

Screening Criteria: Yes

Technical Criteria: Yes. This project duplicates ongoing work. Redirect funds from the 4 sturgeon proposals (8605000, 9502700, 9056, 9700900) to this project. Project 8605000 agreed to reduce its genetic component if this project gets funded.

Programmatic Criteria: Yes


Recommendation:
Fund
Date:
May 13, 1998

Comment:


Recommendation:
Adequate
Date:
Jun 18, 1998

Comment:

This is an especially good proposal ranked in the top 10 of the set. It contains excellent linkage to the Fish and Wildlife Program measures, and excellent linkage to other fish and wildlife program/BPA projects, both conceptually and in shared samples. We assume the genetic methods are good; the presentation of them is excellent. It is not clear how much genetic difference is a real and significant difference nor is it clear if they will track over an extended time frame. No plans are described to see if the patterns remain stable over time (monitoring).