FY 2000 proposal 20043

Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary

Reviews and Recommendations

Additional documents

Title	Type
20043 Narrative	Narrative

Section 1. Administrative

Proposal title	Intracytoplasmic Sperm Injection: Genetic Retrieval From Single Sperm
Proposal ID	20043
Organization	University of Idaho (UI)
Proposal contact person or principal investigator
Name	Joseph G. Cloud
Mailing address	Moscow, ID 83844-3051
Phone / email	2088856388 / jcloud@uidaho.edu
Manager authorizing this project
Review cycle	FY 2000
Province / Subbasin	Mainstem/Systemwide / Systemwide
Short description	Develop methodology to retrieve the genetics from a single sperm by microinjection into an egg.
Target species	chinook salmon (Oncorhynchus tshawystcha) steelhead (Oncorhynchus mykiss)

Project location

Latitude	Longitude	Description

Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agency	Action #	BiOp Agency	Description

Section 2. Past accomplishments

Year	Accomplishment

Section 3. Relationships to other projects

Project ID	Title	Description
9703800	Listed Salmonid Stocks Gamete Preservation	ICSI of salmonid sperm will interact with the development of a sperm bank by ultimately providing insurance against accidental thawing of the cryopreserved materials
	Enhancement of Samonid Gamete Quality by Manipulation of Intracellualar ATP	CO-PI; both projects have a goal of increasing the probability of retrieving the genetic information from sperm
	Endocrine Control of Ovarian Development in Salmonids	A participant of the UI/WSU Fish Reproduction Program
	Analyzing Genetic and Behavioral Changes During Samonid Domestication	A participant of the UI/WSU Fish Reproduction Program
	Induction of Precocious Sexual Maturity and Enhanced Egg Production in Fish	A participant of the UI/WSU Fish Reproduction Program
	Viral Vaccines and Effects on Reproductive Status	A participant of the UI/WSU Fish Reproduction Program

Section 4. Budget for Planning and Design phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2000 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2000 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Construction and Implementation phase

Section 6. Budget for Operations and Maintenance phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2000 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2000 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget

Item	Note	FY 2000 cost
Personnel	PI (4.0 mo); Scientific Aide (12 mo); Graduate Student (9.0 mo); Undergrad (320 hrs)	$77,696
Fringe	28.5% for PI and Scientific Aide; 1.0% for students	$16,940
Supplies		$24,000
Operating	equipment repair	$4,240
Capital		$8,765
Travel		$3,000
Indirect		$69,124
Other	Administrative and Aquaculture Core Facilities	$20,000
		$223,765

Total estimated budget

Total FY 2000 cost	$223,765
Amount anticipated from previously committed BPA funds	$0
Total FY 2000 budget request	$223,765
FY 2000 forecast from 1999	$0
% change from forecast	0.0%

Cost sharing

Organization	Item or service provided	Amount	Cash or in-kind

Other budget explanation

Schedule Constraints: None

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

REVIEW:
ISRP Preliminary Review (ISRP 99-2)

Recommendation:
Do Not Fund

Date:
Jun 15, 1999

Comment:

Recommendation: Do not fund.

Comments: This proposal specifically addresses future incorporation of genetic material from presently infertile males into future generations. This is an expensive proposal to address the disadvantages to the (present) cryopreservation approach to conserving genetic material, and should be examined from the standpoint of the investment in a strategy that might be considered a technological alternative to restoring spawning populations. Reviewers find no convincing argument as to how the proposed research relates to priority needs of the FWP. The proposal does not adequately describe how it will be programmatically applied. There is no citation of other applications of similar technology to other ESA efforts.

REVIEW:
CBFWA Funding Recommendation

Recommendation:
Do Not Fund

Date:
Aug 20, 1999

Comment:

REVIEW:
CBFWA: Nonwatershed Technical Group Comments

Recommendation:

Date:
Aug 20, 1999

Comment:

Criteria all: Met? yes - Question the applicability of this research.

REVIEW:
CBFWA: Subregional Team Comments

Recommendation:

Date:
Aug 20, 1999

Comment:

Sounds like pure theoretical research. Unclear what the application is for the recovery of listed species.

REVIEW:
NWPPC Funding Recommendation (NWPPC 2000-6)

Recommendation:
Do Not Fund

Date:
Mar 1, 2000

Comment:

[Decision made in 9-22-99 Council Meeting];