FY 2000 proposal 20045

Additional documents

TitleType
20045 Narrative Narrative

Section 1. Administrative

Proposal titleAnalyzing Genetic and Behavioral Changes During Salmonid Domestication
Proposal ID20045
OrganizationWashington State University (WSU)
Proposal contact person or principal investigator
NameGary H. Thorgaard
Mailing addressDepartment of Zoology Pullman, WA 99164-4236
Phone / email5093357438 / thorglab@wsu.edu
Manager authorizing this project
Review cycleFY 2000
Province / SubbasinMainstem/Systemwide / Systemwide
Short descriptionAnalyze genetic changes occurring during domestication in chinook salmon and steelhead trout by studying selection on mapped DNA markers under wild and hatchery conditions and analyze behavioral and physiological changes using standardized tests.
Target speciesChinook salmon (Oncorhynchus tshawytscha), Steelhead trout (Oncorhynchus mykiss)
Project location
LatitudeLongitudeDescription
Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agencyAction #BiOp AgencyDescription

Section 2. Past accomplishments

YearAccomplishment

Section 3. Relationships to other projects

Project IDTitleDescription
Intracytoplasmic Sperm Injection: Genetic Retrieval from Sperm also part of UI/ WSU Fish Reproduction program grant
Endocrine Control of Ovarian Development in Salmonids also part of UI/ WSU Fish Reproduction program grant
Induction of Precocious Sexual Maturity and Enhanced Egg Production in Fish also part of UI/ WSU Fish Reproduction program grant
Motility and Fertility of Salmonid Gametes also part of UI/ WSU Fish Reproduction program grant
Viral Vaccines and Effects on Reproductive Status also part of UI/ WSU Fish Reproduction program grant

Section 4. Budget for Planning and Design phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2000 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2000 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Construction and Implementation phase

Section 6. Budget for Operations and Maintenance phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2000 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget
ObjectiveTaskDuration in FYsEstimated 2000 costSubcontractor
Outyear objectives-based budget
ObjectiveStarting FYEnding FYEstimated cost
Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget
ItemNoteFY 2000 cost
Personnel .5 mo salary (summer) for 3 co-Pis; 2 postdoc fellows (1 yr), 1 sem. Support for grad research asst $70,924
Fringe Benefits for the above six employees $20,874
Supplies Molecular biology supplies for mapping studies ($15K), behavior testing ($3K), hormone testing ($6K) $24,000
Capital PCR machine for genetic mapping studies ($4,000), Video equipment for behavior studies ($10,000) $14,000
Travel Travel to collect gametes and attending a scientific meeting $1,000
Indirect 45% of direct costs excluding equipment and student tuition $59,922
Other Costs of aquaculture core lab ($15K) and admin core for Fish Reproduction Program ($4K) $19,000
$209,720
Total estimated budget
Total FY 2000 cost$209,720
Amount anticipated from previously committed BPA funds$0
Total FY 2000 budget request$209,720
FY 2000 forecast from 1999$0
% change from forecast0.0%
Cost sharing
OrganizationItem or service providedAmountCash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

Recommendation:
Fund
Date:
Jun 15, 1999

Comment:

Recommendation: Fund, OK for a multi-year review cycle, review in FY2002 for results to date.

Comments: Rationale. There is evidence that hatcheries domesticate salmon, which is manifested in changed behavior and physiology. A consequence of domestication expected is that offspring of wild salmon and hatchery products will be less fit in the wild because they will have inherited maladaptive traits from less fit hatchery parents.

This project proposes to develop readily observed indices of domestication, which are behavioral assay, cortisol assay, and fluctuating asymmetry. These indices would serve resource managers as a means of evaluating specific stocks. They propose to use QTL techniques to map these domestication traits on the genome of steelhead and chinook. They'll develop a microsatellite map for chinooks similar to the one Thorgaard has for rainbow; entails producing inbred androgens, which Thorgaard has done for rainbows. They'll develop behavior and physiological and meristic (FA) stress indicators that hypothetically relate to domestication selection. They will test for associations between traits and genetic map. It is not explicitly claimed, but the ambition seems to be to be able to assess the 'domestication' of a group of salmon by assessing the frequencies of QTL's known to be associated with domestication traits.

The method entails working with pairs of chinook and steelhead stocks, each pair containing domesticated and wild. A product useful to the FWP will be "standard behavioral tests that can be used to monitor levels of domestication" of those species; it is not clear how the information would be used in future hatchery management. (One reviewer suggests that behavioral work be conducted in running water rather than static conditions.)

The proposers are eminent in their respective disciplines and provide considerable evidence of peer-reviewed publications of their work. This is highly innovative science. The ISRP strongly endorsed this project and recommends it for funding.


Recommendation:
Do Not Fund
Date:
Aug 20, 1999

Comment:


Recommendation:
Date:
Aug 20, 1999

Comment:

Criteria all: Met? yes - Strategy of ongoing programs is to avoid domestication. Possible overlap with #9005200.
Recommendation:
Date:
Aug 20, 1999

Comment:

Sounds like pure theoretical research. Unclear what the application is for the recovery of listed species.
Recommendation:
Rank 1
Date:
Oct 8, 1999

Comment:

Rank Comments: This proposal is for highly innovative science that should assist in the development of hatchery performance standards such as those being developed for the Council's Artificial Production Review. It addresses all four of the Council criteria. This proposal will provide useful information: 1) in the application and use of supplementation which is a major part of the Council's program, and 2) in setting policies in regard to interactions of wild and hatchery fish.
Recommendation:
Rank 1
Date:
Oct 8, 1999

Comment:

This proposal is for highly innovative science that should assist in the development of hatchery performance standards such as those being developed for the Council's Artificial Production Review. It addresses all four of the Council criteria. This proposal will provide useful information: 1) in the application and use of supplementation which is a major part of the Council's program, and 2) in setting policies in regard to interactions of wild and hatchery fish.
Recommendation:
Fund as Innovative
Date:
Mar 1, 2000

Comment:

27. Projects recommended by ISRP, but rated tier 2 or tier 3 by CBFWA/Innovative projects.

There are two groups of projects that the Council considered for funding. First, the ISRP recommended projects for funding that were rated as either tier 2 or tier 3 by CBFWA (the "elevated projects"). Two law enforcement projects were added to this "elevated" list because they did not receive a funding recommendation from CBFWA, but were rated as "fund" by the ISRP. The second group of projects are those that the ISRP identified in its report as "innovative" and offering promising new techniques or approaches (the "innovative projects").

All of the projects that the ISRP found to be "innovative" (and also meeting the scientific review standards) were included in first list of "elevated" projects by the ISRP. The Council itself did not combine the project lists.

In past reports, the ISRP has expressed concern that new and innovative project proposals were not receiving sufficient attention in the funding process. Two years ago, the Council created a targeted request for proposals process for certain areas of interest that had not otherwise received funding recommendations, and a relatively small amount of funding was provided for qualifying projects. The Fiscal Year 2000 solicitation for proposals indicated that an "innovative proposal fund" would be established to support new initiatives of this type.

However, no criteria were specified for "innovative" proposals and most new projects were not proposed as "innovative." The Council requested that the ISRP prioritize the list of "elevated" projects (42 total). The Council also asked the ISRP to consider four specific criteria in its rankings. They were asked to determine if the project: 1) dealt with an unimplemented program area; 2) improves existing projects; 3) has systemwide significance; and 4) advances critical watershed assessment work. The ISRP ranked the projects from 1 to 42 based on their assessment of the overall worth of each project and indicated which of the criteria were met by each. The Council reviewed the ranked list of 42 projects, and determined that it would not recommend funding for all of them. The Council established $2 million as a planning target for funding projects on this list. In order to bring discipline to the selection process, the Council decided what type of projects it wanted to recommend the limiting funding for. The Council determined that it wished to focus on research-oriented projects that the ISRP found to be innovative, and also met two or more of the four criteria identified above (as determined by the ISRP). At the February 1, 2000 work session meeting in Portland, the Council recommended possible funding for eleven projects from the list of 42 elevated projects. Those projects are:

20045, 20057, 20034, 20102, 20106, 9803500, 20064, 20006, 20067, 20076, and 20054.

Review of the ISRP rankings shows that only these projects were identified by the ISRP as fulfilling an unimplemented program area and having systemwide significance. These 11 projects were mainly in the upper half of the overall ranking; the lowest-ranked project on the list ranks 24 out of 42. All 11 projects are research-oriented and, by definition, fulfill part of our current fish and wildlife program and have importance for the system as a whole. The Council found that this seems a reasonable subset of projects to be funded as "innovative."

The Council has previously indicated its desire to cover all of the initial costs for "innovative" projects at the time they are selected, allowing a new competition for funding of innovative projects to be held each year without creating a burden on future years' budgets. Unfortunately, the proposed budgets for these eleven projects, over the next four years, would exceed $8 million. (The budgets for the first four projects alone would exceed $4 million.) Rather than fully fund a few projects, the Council's proposal is to provide initial funding for preliminary research, prototyping, and proof of concept for all 11 projects. Specifically, the proposal is to offer each project $200,000 (or the amount initially requested by the sponsor if that amount is less than $200,000), for a total of $2,119,000. After completion of the initial work and a final report on that work, project sponsors would be free to seek additional funding as a part of the regular project selection process.

While $200,000 is much less than the sum requested for most of these projects, it is still a substantial amount by the standards of most research grants and should lead to meaningful results. This approach also allows us to gain further information on the value of research before making a large, long-term investment.

Project sponsors designated to receive this funding are being asked to prepare a revised plan of work reflecting the reduced funding. The revised plan would be reviewed by Council staff and the chairman of the ISRP to assure that the revised plan still represents valuable research that is consistent with the proposal originally reviewed by the ISRP. In summary, the staff proposal is as follows:

After the Council and ISRP representatives review the revised plans for the eleven projects noted above, and confirm that valuable innovative research can be conducted and reported under the funding and other conditions discussed above, the Council will advise Bonneville under separate cover of its final recommendations for these projects. The Council anticipates that it can provide final recommendations for these projects to Bonneville in late March. Bonneville should refer to that separate letter on this issue for the final Council recommendations on these projects.

Note: Unless the context indicates otherwise, "fund" means that the Council would recommend to the Bonneville Power Administration that a project be funded. The Council's fish and wildlife program is established by statute for implementation by Bonneville, and the Council itself does not directly fund fish and wildlife mitigation. However, in recent years, Bonneville has followed the Council recommendations closely.


Recommendation:
Fund
Date:
Mar 1, 2000

Comment:

[Decision made in 2-2-00 Council Meeting]; Eligible for $200,000 as an innovative project