FY 2001 Ongoing proposal 199009300

Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary

Reviews and Recommendations

Additional documents

Title	Type

Section 1. Administrative

Proposal title	Genetic Analysis of Oncorhynchus nerka (Modified to include Chinook Salmon)
Proposal ID	199009300
Organization	University of Idaho (UI)
Proposal contact person or principal investigator
Name	Madison S. Powell
Mailing address	3059F National Fish Hatchery Road Hagerman, ID 83332
Phone / email	2088379096 / fishdna@micron.net
Manager authorizing this project	Madison S. Powell
Review cycle	FY 2001 Ongoing
Province / Subbasin	Systemwide / Systemwide
Short description	This project provides genetic information on O. nerka and O. tshawytscha throughout the Snake and Columbia Basins to be used in the overall recovery and captive propagation of endangered Snake River sockeye and threatened Salmon River chinook salmon.
Target species

Project location

Latitude	Longitude	Description

Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA

Relevant RPAs based on NMFS/BPA review:

Reviewing agency	Action #	BiOp Agency	Description

Section 2. Past accomplishments

Year	Accomplishment
1995	Genetic Relationships Between Maternal lineages of Sympatric Anadromous and Resident Forms of Oncorhynchus nerka determined through PCR-RFLP Analysis of mtDNA submitted as partial fulfillment of the requirement for a Master of Science degree, U of I.
1995	Development of a standardized mitochondrial DNA Restriction Fragment Length Polymorphism assay for 4 gene regions in sockeye salmon.
1995	Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1995	Genetic Analysis of Oncorhynchus nerka. Annual Report to BPA.
1996	Presentation of Redfish Lake sockeye mitochondrial data at 2 invited symposia.
1996	Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1997	Presentation of Redfish Lake sockeye mitochondrial data at BPA review.
1997	Expansion of genetic diversity analyses among sockeye to include 37 populations (including kokanee).
1997	Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1998	Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1998	Construction and analysis of a genetic breeding matrix used for captive East Fork salmon River chinook salmon.
1999	Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1999	Genetic Analysis of threatened Salmon River Chinook Salmon (Oncorhynchus tshawytscha) Populations. Technical Report.
1999	Determination of genetic identities of returning Redfish Lake Captive Broodstock program adults and returning Sawtooth Hatchery chinook salmon.
1999	Construction and analysis of a genetic breeding matrix used for captive West Fork, Yankee Fork and Sawtooth chinook salmon.
2000	Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
2000	Phylogeography of Oncorhynchus nerka in the Pacific Northwest, resubmitted for peer-reviewed publication.
2000	Review of Redfish Lake O.nerka Populations and Efforts to Recover Endangered Redfish Lake Anadromous Sockeye, submitted as partial fulfillment of the requirement for a Master of Science degree, University of Idaho.
2000	Final report for sockeye mitochondrial DNA diversity and Columbia Basin phylogeography submitted to BPA (May)

Section 3. Relationships to other projects

Project ID	Title	Description

Section 4. Budget for Planning and Design phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost
1. Monitor and evaluate Snake River O. nerka populations.	a. Examine all returning sockeye, creel surveys, and trawls using mtDNA (FY2001)	.15	$19,585
1.	b. Examine all returning sockeye, creel surveys and trawls using 4 microsatellite markers (all years).	.25	$32,641
1	c. Analyze available samples from Snake and Columbia River sockeye with 4 microsatellite markers.	.15	$19,585
2. Continue to assess mitochondrial DNA variation among Salmon River Chinook Salmon	a. Complete mitochondrial DNA RFLP analysis of Salmon River Chinook Populations.	.05	$6,528
2.	b. Compare Salmon River Chinook Populations with current data from other Columbia Basin populations.	.1	$13,055
3. Continue to assess nuclear genetic variation among Salmon River Chinook salmon	a. Continue microsatellite DNA analysis of Salmon River Chinook Salmon.	.15	$19,585
3.	b. Utilize microsatellite analysis for relatedness and pedigree evaluation of captive chinook salmon.	.15	$19,585

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Construction and Implementation phase

FY 2004	FY 2005	FY 2002	FY 2003
$65,000	$65,000	$65,000	$65,000

Section 6. Budget for Operations and Maintenance phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2001 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget

Item	Note	FY 2001 cost
Personnel	FTE: Principle Investigator: 0.5 FTE Research Support Scientist: 1.0 FTE	$58,344
Fringe	Principle Investigator @0.285 Res. Support Scientist@0.285	$16,628
Supplies	chemicals, pipet tips, gloves, labelled primers	$18,100
Travel	1 professional meeting (AFS), and travel to 12 monthly T.O.C. meetings for 1 person.	$3,200
Indirect	Off campus indirect cost rate @ 20.7%	$22,392
Capital	Stratagene thermocycler and a 24 place microfuge	$11,900
		$130,564

Total estimated budget

Total FY 2001 cost	$130,564
Amount anticipated from previously committed BPA funds	$0
Total FY 2001 budget request	$130,564
FY 2001 forecast from 2000	$145,000
% change from forecast	-10.0%

Reason for change in estimated budget

Microsatellite analysis will be conducted off campus at the Hagerman Fish Culture Experiment Station instead of the laboratory at Moscow. Thus, the off campus indirect cost rate (20.7%) applies instead of an indirect rate of 47%.

Reason for change in scope

Cost sharing

Organization	Item or service provided	Amount	Cash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

REVIEW:
CBFWA Funding Recommendation

Recommendation:
Ongoing Funding: yes; New Funding: no

Date:
Jul 14, 2000

Comment:

There is no change in scope of work. The reduction in budget is due to cost cutting measures. Microsatellite analysis will be conducted off campus at the Hagerman Fish Culture Experiment Station instead of the laboratory at Moscow. Thus, the off campus indirect cost rate (20.7%) applies instead of an indirect rate of 47%.

REVIEW:
NWPPC Funding Recommendation

Recommendation:
Fund

Date:
Sep 13, 2000

Comment:

REVIEW:
NW Power and Conservation Council's FY 2006 Project Funding Review

Funding category:
expense

Date:
May 2005

FY05 NPCC start of year:	FY06 NPCC staff preliminary:	FY06 NPCC July draft start of year:
$126,000	$98,000	$98,000

Sponsor comments: See comment at Council's website