Return to Proposal Finder FY 2003 Columbia Estuary Proposal 30013

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget Summary

Reviews and Recommendations
Title Type File Size File Date


Section 1. General Administrative Information

Title of Project Proposal Role of Bacteria as Indicator Organisms for Watershed Assessment and in Determining Fish Pathogen Relationships with Fauna of Abernathy Creek
BPA Project Proposal Number 30013
Business name of agency, institution,
or organization requesting funding
U.S. Fish and Wildlife Service - Abernathy Fish technology Center
Business acronym (if appropriate) USFWS
 

Proposal contact person or principal investigator

Name Richard Glenn
Mailing Address 1440 Abernathy Creek Road
City, State, Zip Longview, WA. 98632
Phone 3604256072
Fax 3606361855
E-mail richard_glenn@fws.gov
 
Manager of program authorizing this project Carl Burger
 
Review Cycle Columbia Estuary
Province Columbia Estuary
Subbasin Elochoman
 
Short Description The purpose of this project is to develop techniques to assess watershed health and fish health using bacteria as system indicator organisms.
Target Species Steelhead Trout (Onchorhynchus mykiss), Cutthroat Trout (Onchorhynchus clarki), Aquatic Ecosystem


Project Location

Latitude Longitude Description
46.11'15" 123.08'40" Headwater of Abernathy Creek
46.18'45" 123.13'30" Mouth of Abernathy Creek
46.13'00" 123.08'45" Abernathy Fish Technology Center


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

RPA
Action 1
Action 152
Action 155
Action 198

Relevant RPAs based upon NMFS & BPA Review

This information comes from National Marine Fisheries Service (NMFS) and Bonneville Power Administration (BPA) reviews of this project proposal. The following table represents BPA & NMFS determinations as to this proposal's relevance to particular Biological Opinion Reasonable and Prudent Alternatives (RPAs).

Reviewing Agency Action Number BiOp Agency Description
NMFS Action 152 NMFS The Action Agencies shall coordinate their efforts and support offsite habitat enhancement measures undertaken by other Federal agencies, states, Tribes, and local governments by the following:


Information Transfer

The expected outcomes of this project are:
Quantitative

Data generated by this project are:
Primary

Are there restrictions on the use of this data?
None

Where do the data reside?
Private/Managed Locally:

Printed
Electronic

Public Access: [no information]


In what other ways will information from this project be transferred or used?

It will be used to show the strengths of bacterial testing to indicate health of aquatic system and relate fish disease to environmental fluctuations.


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: Revised budget submitted with response to ISRP on 3/15/02
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

n/a or no information


Section 3. Relationships to Other Projects

Project ID Title Description
30008 The use of small stream PIT tag monitoring methods to evaluate movements, life history charachteristics and survival of salmonid. Will collect fish tissue samples from sacrificed fish.
RPA Action 1 Is a USFWS proposed project which provides research, monitoring, and evaluation of hydro resources and habitat.
RPA Action 152 Federal agency proposed project that will aid in water quality and biological monitoring information for subbasin and watershed assessments.
RPA Action 155 Project will aid to determine cause and effect relationships and identify research needs for improvement plans.
RPA Action 198 Is a USFWS project that will provide data for management of water quality and habitat use and restoration.


Section 4. Budget for Planning and Design Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2003 cost Subcontractor
1. Determine bacterial species found in the water column that are of particular interest. a. Collect water samples, plate, and isolate specific bacteria. 1 $ 8,850  
b. Send isolates to Microbial ID, Inc. for fatty-acid analysis and positive identification. 1 $ 1,000 x
2. Develop 16s rDNA PCR primers a. Use PCR techniques to cut out the 16s rDNA segment. b. Sequence 16s rDNA to determine areas where species specific primers can be developed. 1 $ 18,000  
c. Production of primers at Genosys Biotechnologies. 1 $ 450 x
3. Determine which bacterial species found in the water column are associated with mayfly, stone fly larvae and crawfish a. Capture representative samples of invertebrates. b. Isolate bacteria from their intestines and body cavities and process specific bacteria. 1 $ 9,650  
4. Determine which bacterial species found in the water column and associated with invertebrates are associated with fish populations a. Capture a representative sample of fish of each species of interest and sample intestinal tract and other body cavities and surfaces and process specific bacteria for isolation. 1 $ 9,650  


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Planning and Design Phase

n/a or no information


Section 5. Budget for Construction and Implementation Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Construction and Implementation Phase

n/a or no information


Section 6. Budget for Operations and Maintenance Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Operations and Maintenance Phase

n/a or no information


Section 7. Budget for Monitoring and Evaluation Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2003 cost Subcontractor
1. Obtain overall view of specific bacteria presence in the creek and watershed a. Take water samples at predetermined locations to determine presence of specific indicator bacteria by PCR analysis with species-specific primers. 3 $ 11,300  
b. Produce species-specific primers at Genosys Biotechnologies, Inc. 3 $ 450 x
2. Determine the bacterial relationships between bacteria, invertebrates, and fish species of interest. a. Take tissue samples of invertebrates and fish at the beginning and end of test periods by PCR analysis with species-specific primers. 3 $ 11,300  
b. Produce species-specific primers at Genosys Biotechnologies, Inc. 3 $ 450 x
3. Relate bacterial presence with water quality and environment data a. At sites and in the lab, run water quality analysis and obtain water environment data 3 $ 2,350  
4. Relate bacterial presence and environment data to land uses. a. Use survey maps to assess land uses and environment changes. 3 $ 0  


Outyear Objective-Based Budget

Objective Starting FY Ending FY Estimated cost
1. Obtain an overall view of specific bacteria and their presence in the creek. 2004 2005 $ 59,300
2. Determine the bacterial relationships between bacteria, invertebrates, and fish species of interest. 2004 2005 $ 59,300
3. Relate bacterial presence with water quality and environment data. 2004 2005 $ 500


Outyear Budgets for Monitoring and Evaluation Phase

FY 2004 FY 2005
$ 58,690 $ 60,400


Section 8. Estimated Budget Summary

Itemized Budget

Item Note FY 2003 Cost
Personnel FTE: GS-5 $ 25,700
Fringe GS-5 health/dental/life/retirement $ 8,500
Supplies Laboratory and Sampling Supplies $ 13,000
Travel Seminars and conferences $ 2,000
Indirect Overhead costs $ 17,900
Subcontractor Microbial ID, Inc. $ 1,000
Subcontractor Genosys Biotechnologies, Inc $ 1,350
Other Sequencer Training $ 4,000
Total Itemized Budget $ 73,450


Total estimated budget

Total FY 2003 project cost $ 73,450
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2003 budget request $ 73,450
FY 2003 forecast from 2002 $ 0
% change from forecast 0.0%


Reason for change in estimated budget

Not applicable


Reason for change in scope

Not applicable


Cost Sharing

Not applicable
 

Outyear Budget Totals

2004 2005
Monitoring and evaluation $ 58,690 $ 60,400
Total Outyear Budgets $ 58,690 $ 60,400
 

Other Budget Explanation

Not applicable


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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