Return to Proposal Finder FY 1999 Proposal 9084

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date


Section 1. General Administrative Information

Title of Project Proposal Assessing Genetic Variation Among Columbia Basin White Sturgeon Populations
BPA Project Proposal Number 9084
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI
 

Proposal contact person or principal investigator

Name Madison S. Powell
Mailing Address 3059 F National Fish Hatchery Road
City, State, Zip Hagerman, ID 83332
Phone 2088379096
Fax 2088376047
E-mail fishdna@micron.net
 
Manager of program authorizing this project
 
Review Cycle FY 1999
Province Systemwide
Subbasin Systemwide
 
Short Description Genetic variation and stock structure among white sturgeon populations in the Columbia Basin based on analysis of mitochondrial and nuclear DNA.
Target Species


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


NPPC Program Measure Number(s) which this project addresses: 10.4, 10.4A.1 through 10.4A5, 10.6C, 10.6C.1, 10.8B15, 10.8B16.
FWS/NMFS Biological Opinion Number(s) which this project addresses: Kootenai River White Sturgeon Aquaculture Program (DOE-EA-1169), USFWS 1994 Biological Opinion on the 1994-1998 Federal Columbia River Power Operation Assessment, USFWS, Biological Opinion 1995 1-4-95-F-003 (Kootenai River White Sturgeon)
Other Planning Document References


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): resident


Section 2. Past Accomplishments

n/a or no information


Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
White sturgeon mitigation and restoration in the Columbia and Snake Rivers This project will provide genetic information for management. The 86-50 project provides tissue samples for this project. No
Kootenai River ecosystem and fisheries improvement study This project will provide genetic information on an endangered population. The 94-49 project provides tissue samples for this project. No
Kootenai River fisheries studies This project will provide genetic information on an endangered population. The 88-64 project provides tissue samples for this project. No
Kootenai river fisheries investigations This project will provide genetic information on an endangered population. The 88-65 project provides tissue samples for this project. No


Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Preliminary assessment of genetic variation among Columbia Basin white sturgeon a. Mitochondrial D-loop length variation will be described and compared pair-wise between geographically proximate samples and amon geographically separated samples.
2. Assessment of mitochondrial sequence divergence among Columbia Basin white sturgeon. a. Sequence divergence of a non-repetitive portion of the D-loop will be compared among samples from each location listed in the first objective.
3. Assessment of nuclear genetic variation among Columbia Basin white sturgeon populations a. Eight nucleotide primer pairs for microsatellite loci will be used to examine nuclear genetic variation in the same populations listed previously.

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 01/01/99 12/01/99 18.0%
2 06/01/99 05/01/00 32.0%
3 06/01/99 12/01/01 50.0%


Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 1999 Cost
Personnel M. Powell, 6 mo. @ 24.00/hr. (1040 h) P. Anders, 12 mo. @ 16.90/hr. (2080 h) $ 60,112
Fringe 28.5% for both $ 17,132
Supplies chemicals, pipet tips, tubes, gloves etc. $ 20,000
Operating Equipment service and calibration, UPS shipping, Federal Express, long distance calls/faxing $ 2,000
Capital 1 Power Macintosh Computer and Perkin Elmer ABI Genotyper Software $ 11,000
Travel 1 professional meeting (AFS), 2 people, travel between laboratories $ 1,500
Indirect off campus indirect cost @ 25.8% $ 25,992
Subcontractor $ 0
Total Itemized Budget $137,736


Total estimated budget

Total FY 1999 project cost $137,736
Amount anticipated from previously committed BPA Funds $ 0
Total FY 1999 budget request $137,736
FY 1999 forecast from 1998 $ 0
% change from forecast 0.0%


Reason for change in estimated budget

Not applicable


Reason for change in scope

Not applicable


Cost Sharing

Not applicable
 

Outyear Budget Totals

2000 2001
All Phases $148,000 $152,000
Total Outyear Budgets $148,000 $152,000
 

Other Budget Explanation

Schedule Constraints: Difficult sequencing or equipment breakdown for automated sequencing may lengthen the time required to complete Objective #2 and some of Objective #3. Projections for completion are: Objective #1; 1999, Objective #2; 2000, Objective #3; 2001


Section 6. References

n/a or no information


Section 7. Abstract

Abstract

The genetic relationships of white sturgeon (Acipenser transmontanus) populations within the Columbia Basin remain unclear. To date, there has been no comprehensive genetic assessment of sturgeon populations in the Columbia, Snake and Kootenai River systems using any analytical method. The objective of this project is to assess inter- and intrapopulational genetic variation among white sturgeon in the Columbia, Snake, and Kootenai River Basins. This project will employ both nuclear and mitochondrial DNA analyses to test the null hypothesis; white sturgeon populations in the Columbia Basin represent (a) a single gene pool, and (b) one ESU of the species. This project is essential to most aspects of the Columbia Basin Fish and Wildlife Program and directly addresses section 10.4A “Study and evaluate Sturgeon Populations.” For example, falsification of this hypothesis will critically effect the management of white sturgeon populations within these watersheds. Divergent populations will have to be considered for management as significant components of overall white sturgeon diversity, possibly requiring additional measures, such as supplementation, to ensure their long term stability or recovery. It is expected that this comprehensive genetic assessment will require three years to complete. The results will be reviewed and assessed by those currently involved in BPA funded white sturgeon research and recovery throughout the Columbia Basin.


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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