Return to Proposal Finder FY 1999 Proposal 9143

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Evaluate Disease Interactions Between Wild and Hatchery Salmonids
BPA Project Proposal Number 9143
Business name of agency, institution,
or organization requesting funding
Oregon State University, Department of Microbiology
Business acronym (if appropriate) OSU

Proposal contact person or principal investigator

Name Dr. Linda M. Bootland
Mailing Address Rm 528 Nash Hall, Dept. Microbiolgy, Oregon State Univ.
City, State, Zip Corvallis,OR 97331
Phone 5417371860
Fax 5417370496
Manager of program authorizing this project
Review Cycle FY 1999
Province Systemwide
Subbasin Systemwide
Short Description This research addresses the role disease plays in the survival of wild salmon and whether hatchery fish negatively impact wild fish either directly, by transmitting pathogens and disease, or indirectly, by reducing the fitness of wild fish.
Target Species

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 7.2A.2, 7.2D.4
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

n/a or no information

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Determine the prevalence of pathogens and disease in wild salmonids in the CRB a. Complete a literature search to compile information on pathogens and diseases of fish in the CRB
. b. Survey of pathogens in salmonid smolts
2. Determine whether hatchery and wild fish show differences in disease susceptibility a. Obtain gametes from wild and hatchery stocks from selected locations in the CRB
. b. Quantify genetic divergence between populations
. c. Compare the susceptibility of fish from each stock to a viral, parasitic and bacterial fish pathogen
. d. Determine the effect of environmental parameters on the survival of pathogen-exposed fish
3. Determine whether fish surviving disease can transmit infection and disease to naive fish a. Determine if cohabitation of naive and infected fish results in pathogen transmission
. b. Determine if disease survivors can transmit infection to naive fish when held under stressful conditions
4. Determine if interbreeding between hatchery and wild fish causes a change in disease resistance a. Produce F1 crossbred progeny between hatchery and wild fish.
. b. Expose F1 progeny to pathogens
. c. Characterize genetic differences in the F1 progeny

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 10/01/98 02/01/02 20.0%
2 10/01/98 12/01/00 30.0%
3 05/01/99 10/01/01 20.0%
4 10/01/00 09/01/02 30.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 1999 Cost
Personnel Bootland $27,756, Bartholomew $12,336, RA @.33 FTE $15,688, 2 RA $52,308 $108,088
Fringe @41% to 51% $ 49,623
Supplies PCR reagents, tissue culture reagents, ELISA kits, plasticware $ 24,000
Travel local travel to collection sites, partial travel to one national meeting for each PI $ 3,000
Indirect @42.5% $ 84,877
Subcontractor USFWS $ 15,000
Total Itemized Budget $284,588

Total estimated budget

Total FY 1999 project cost $284,588
Amount anticipated from previously committed BPA Funds $ 0
Total FY 1999 budget request $284,588
FY 1999 forecast from 1998 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2000 2001 2002
All Phases $294,405 $285,820 $299,376
Total Outyear Budgets $294,405 $285,820 $299,376

Other Budget Explanation

Not applicable

Section 6. References

n/a or no information

Section 7. Abstract


The importance of maintaining genetic diversity in the salmon stocks of the Columbia River Basin (CRB) is well recognized, but little attention has been paid to the relationship between genetic diversity and disease resistance in wild salmonid populations. It has been assumed that hatchery fish cause disease in wild fish and that interbreeding of hatchery fish with wild fish may cause adverse genetic changes, including loss of disease resistance. However, there is little scientific evidence to support either of these assumptions. The goal of the proposed research is provide data that will establish whether or not hatchery fish adversely affect the disease status of wild fish. Objectives have been designed to determine whether 1) there are significant differences between hatchery and wild salmonid populations in infection and disease resistance, 2) acutely infected hatchery fish can transmit infection and disease to wild salmonids, 3) hatchery fish that survive infection can transmit pathogens and disease to wild fish when held under stressful conditions, and 4) interbreeding of hatchery fish with wild salmonids adversely affects the genetically-controlled disease resistance of progeny. Answers to these questions will be obtained over four years and will be of value in making policy and management decisions related to the use of hatchery fish within the CRB. The proposed research addresses measures 7.2A.2 and 7.2D.4 in the Fish and Wildlife Program by providing scientific data necessary to increase salmon runs in a biologically sound manner.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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