Return to Proposal Finder FY 1999 Proposal 9150

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Nutritional Status of Columbia River White Sturgeon
BPA Project Proposal Number 9150
Business name of agency, institution,
or organization requesting funding
Oregon State University, Department of Food Science and Technology
Business acronym (if appropriate) OSU

Proposal contact person or principal investigator

Name Jan M. Spitsbergen
Mailing Address Weigland Hall
City, State, Zip Corvallis, OR 97331
Phone 5417375055
Fax 5417376074
Manager of program authorizing this project
Review Cycle FY 1999
Province Lower Columbia
Subbasin Lower Columbia Mainstem, Lower Mid-Columbia Mainst
Short Description Investigate the possible role of nutritional factors in reproductive dysfunction and early life stage mortality problems limiting recruitment to white sturgeon populations in the Columbia River above Bonneville Dam.
Target Species

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 10.4 Sturgeon Mitigation
FWS/NMFS Biological Opinion Number(s) which this project addresses: Though this project does not propose to study the Endangered Kootenai River Population of White Sturgeon, the findings of this project may benefit restoration efforts for the Kootenai Sturgeon.
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): resident

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
8605000 White Sturgeon Productivity Status and Habitat Requirements Source of young of year sturgeon No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Determine levels of micronutrients and vitamins in serum and liver of adult white sturgeon from the Columbia R. above and below Bonneville Dam a. Perform ICP-MS analyses for iodine, selenium
. b. Perform HPLC analyses for vitamin E
. c. Use thiochrome method to assess thiamine.
2. Determine levels of certain micronutrients and vitamins in early life stages and juvenile sturgeon above and below Bonneville Dam. a. Perform ICP-MS analyses for iodine, selenium.
. b. Perform HPLC analyses for vitamin E
. c. Use thiochrome method to assess thiamine.
3. Evaluate histology of thyroid of adult and juvenile white sturgeon from the Columbia R. above and below Bonneville Dam. a. Obtain samples of thyroid tissue, fix in formalin, prepare and examine histologic sections.
4. Correlate histologic sessions of thyroid with micronutrient and vitamin status. a. Perform statistical analysis of thyroid histologic and nutritional data to determine correlations.
5. Determine minimal dietary iodine levels, with and without sufficient dietary vitamin E and selenium, to ensure normal thyroid function in juvenile white sturgeon. a. Raise young of the year white sturgeon in the laboratory while feeding diets containing various levels of iodine, selenium and vitamin E.
. b. Assay serum thyroxin (T4) and triiodothyronin (T3) at bimonthly intervals during feeding of diets containing various levels of nutrients.
. c. At midpoint and end of feeding trials, prepare thyroid tissue from sturgeon for histologic examination.
. d. Statistically evalute nutritional influences on thyroid hormone.
6. Determine the influence of iodine and/or vitamin E/selenium deficiency on sensitivity of juvenile white sturgeon to disruption of thyroid function by halogenated aromatic hydrocarbons. a. Raise young of the year white sturgeon in the lab while feeding various levels of iodine and vitamin E/selenium in combination with Arochlor 1254 or a coplanar PCB.
. b. Assay serum T4 and T3 at bimonthly intervals during dietary studies.
. c. At midpoint and end of feeding trials, prepare thyroid tissue from sturgeon for histologic examination.

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 10/01/98 09/01/99 10.0%
2 10/01/98 09/01/99 10.0%
3 10/01/98 09/01/99 70.0%
4 10/01/98 09/01/99 20.0%
5 10/01/99 09/01/01 35.0%
6 10/01/99 09/01/01 36.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 1999 Cost
Personnel Technician, 25% effort, PI, 25% effort $ 21,907
Fringe $ 8,701
Supplies For analyses of Se, I, thiamine, vitamin E; preparation of histology slides $ 42,450
Operating $ 11,821
Travel For sample collection - 10 days x $100/d $ 1,000
Indirect 42.5% $ 31,815
Subcontractor $ 0
Other Publication costs, 1 manuscript $ 800
Total Itemized Budget $118,494

Total estimated budget

Total FY 1999 project cost $118,494
Amount anticipated from previously committed BPA Funds $ 0
Total FY 1999 budget request $118,494
FY 1999 forecast from 1998 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2000 2001 2002 2003
All Phases $112,960 $114,648 $140,000 $143,000
Total Outyear Budgets $112,960 $114,648 $140,000 $143,000

Other Budget Explanation

Schedule Constraints: Difficulty obtaining sufficient numbers of early life stages, young of the year or yearling juvenile sturgeon at all sites for nutritional analysis during the initial year may cause delays of some of those analyses until subsequent years.

Section 6. References

n/a or no information

Section 7. Abstract


This project aims to clarify effects of hydropower projects on nutritional status of white sturgeon in the Columbia River. We plan to investigate levels of micronutrients, antioxidants and vitamins including iodine, selenium, vitamin E and thiamin in adult and juvenile sturgeon in the Columbia River below Bonneville Dam and to compare the nutritional status of these fish to sturgeon in impounded upstream portions of the river. Histological examination of thyroid tissue from fish collected at 5 sites will be used to correlate endocrine status with nutritional status. Dietary investigations in the laboratory using juvenile sturgeon at 2 months post-hatch will assess iodine and vitamin E/selenium requirements for optimal thyroid function and will investigate the influence of dietary deficiency of these nutrients on endocrine disruption by xenobiotics. In these laboratory studies, thyroid endocrine status will be evaluated using both histological parameters and serum thyroid hormone (T3 and T4) levels. In the first year of this study, nutritional status of fish from the Columbia River will be analyzed. In the second and third years of the study, the influence of nutritional parameters on PCB-induced thyroid dysfunction will be evaluated. These studies will yield important insights into the subtle dose-response interactions of diet and xenobiotics in determining endocrine status. Thus these proposed studies will aid in future programs to protect and augment populations of this valuable commercial species in the this river basin. These studies may also suggest novel approaches for increasing recruitment and facilitating restoration of the endangered Kootenai River white sturgeon.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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