Return to Proposal Finder FY 1999 Proposal 198909600

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date


Section 1. General Administrative Information

Title of Project Proposal Monitor, Evaluate Genetic Characteristics of Supplemented Salmon & Steelhea
BPA Project Proposal Number 198909600
Business name of agency, institution,
or organization requesting funding
National Marine Fisheries Service
Business acronym (if appropriate) NMFS
 

Proposal contact person or principal investigator

Name Robin S. Waples
Mailing Address 2725 Montlake Blvd. East
City, State, Zip Seattle, WA 98112
Phone 2608603254
Fax 2068603267
E-mail robin.waples@noaa.gov
 
Manager of program authorizing this project
 
Review Cycle FY 1999
Province Lower Snake
Subbasin Grande Ronde
 
Short Description Monitor changes over time in genetic characteristics of hatchery, natural (supplemented), and wild (unsupplemented) populations of Snake River spring/summer chinook salmon and steelhead. Use results to help evaluate effectiveness of supplementation.
Target Species Snake River spring/summer chinook salmon and steelhead


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


NPPC Program Measure Number(s) which this project addresses:
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: BiOp designation from 4/16/03 budget estimates spreadsheet.
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

n/a or no information


Section 3. Relationships to Other Projects

n/a or no information


Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Collect samples a. Conduct preseason evaluations of previous year escapements to identify optimal sampling strategy
1. Collect samples b. Coordinate sampling efforts to maximum extent possible with other ongoing projects
1. Collect samples c. Collect samples from hatchery, natural, and wild populations
2. Conduct genetic analyses a. Perform allozyme and DNA analyses
2. Conduct genetic analyses b. Perform quality control tests on preliminary data
3. Measure levels of genetic variability in each population a. Quantify percent polymorphic loci, heterozygosity, number of alleles per locus
3. Measure levels of genetic variability in each population b. Compare values in hatchery, natural, and wild populations
3. Measure levels of genetic variability in each population c. Evaluate pattern of change in genetic variability over time
4. Estimate effective population size (Ne) and the ratio Ne/N for each population a. Compute F, a measure of temporal change in allele frequency
4. Estimate effective population size (Ne) and the ratio Ne/N for each population b. Compute r2, a measure of gametic disequilibrium
4. Estimate effective population size (Ne) and the ratio Ne/N for each population c. Use temporal and disequilibrium methods to obtain a combined estimate of Ne for each population
4. Estimate effective population size (Ne) and the ratio Ne/N for each population d. Estimate total population size (N) based on redd counts, spawner surveys, or population enumeration
4. Estimate effective population size (Ne) and the ratio Ne/N for each population e. Compute ratio Ne/N
5. Evaluate population genetic structure of natural and wild populations a. Compute indices of genetic differentiation among natural and wild populations
5. Evaluate population genetic structure of natural and wild populations b. Perform hierarchical gene diversity analyses to partition genetic differences into various components
5. Evaluate population genetic structure of natural and wild populations c. Estimate levels of gene flow among populations based on genetic data
6. Evaluate genetic effects of supplementation on target and non-target populations a. Compute indices of genetic differentiation between hatchery and natural and hatchery and wild populations
6. Evaluate genetic effects of supplementation on target and non-target populations b. Compare patterns of genetic change over time in hatchery populations with those in natural and wild populations
6. Evaluate genetic effects of supplementation on target and non-target populations c. Compare recent genetic data for Grande Ronde populations with historic (pre-supplementation) data obtained from DNA analysis of archived scales
7. Evaluate effectiveness of genetic monitoring a. Quantify genetic differences between hatchery, natural, and wild populations
7. Evaluate effectiveness of genetic monitoring b. Quantify sources of noise in analysis (sampling error, genetic drift)
7. Evaluate effectiveness of genetic monitoring c. In light of a) and b), evaluate combined power of genetic markers (allozymes + DNA) to provide monitoring and evaluation information that is useful for an adaptive management approach to supplementation.

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 08/01/89 09/01/00 10.0%
2 12/01/89 06/01/01 60.0%
3 03/01/90 09/01/01 5.0%
4 06/01/90 09/01/01 5.0%
5 03/01/90 09/01/01 5.0%
6 03/01/90 09/01/01 5.0%
7 03/01/01 03/01/02 10.0%


Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 1999 Cost
Personnel $ 74,200
Fringe $ 32,100
Supplies $ 48,500
Operating $ 3,000
Indirect $ 7,000
Subcontractor $ 45,700
Total Itemized Budget $210,500


Total estimated budget

Total FY 1999 project cost $210,500
Amount anticipated from previously committed BPA Funds $ 0
Total FY 1999 budget request $210,500
FY 1999 forecast from 1998 $ 0
% change from forecast 0.0%


Reason for change in estimated budget

Not applicable


Reason for change in scope

Not applicable


Cost Sharing

Not applicable
 

Outyear Budget Totals

2000 2001 2002 2003
All Phases $250,000 $250,000 $250,000 $250,000
Total Outyear Budgets $250,000 $250,000 $250,000 $250,000
 

Other Budget Explanation

Schedule Constraints: The major potential constraints are availability of parr for sampling from wild and natural populations. This was not a problem in 1989-94, but in 1995 and 1996 lethal collections of spring/summer chinook salmon for allozymes were suspended because of record low returns of adults in 1994 and 1995. In those years, we placed more emphasis on non-lethal fin clips for DNA analysis and continued to sample from hatchery populations as feasible. Lethal sampling in 1997 was restricted to three collections on the South Fork Salmon River. Sampling in 1998 and 1999 should be feasible without undue risk to wild/natural populations, but the abundance of parr in subsequent years is more uncertain. Appropriate levels of sampling will be determined in consultation with state agency biologists, and through the process of securing state and federal ESA collection permits. The recent ESA listing of Snake River steelhead will require an ESA permit for this species.


Section 6. References

n/a or no information


Section 7. Abstract

Abstract

This genetic monitoring program is designed to evaluate the effects of outplanting hatchery-reared fish on natural and wild populations of spring/summer chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss) in the Snake River Basin. The two major goals are 1) to evaluate the nature and extent of genetic changes in hatchery stocks to be used for outplanting, and 2) to quantify the genetic impact of outplanting on targeted natural stocks and non-targeted wild stocks. These goals address important components of the Fish and Wildlife Program, the proposed Snake River Recovery Plan, and other regional planning documents. Information developed in this study will also provide valuable insight into the spatial and temporal scale of genetic structure in natural and wild populations. The basic study plan includes eight different supplementation programs (two for each species). Yearly samples are taken from the hatchery stocks used for supplementation and selected natural and wild populations. Samples are analyzed for a suite of protein and DNA characters, and data obtained will be used to test the hypothesis that the outplanted stock has no significant genetic impact on natural or wild populations. Allele frequency change over time will be used to estimate effective population size and rates of inbreeding. Avoiding deleterious effects of inbreeding is of considerable importance, particularly in stocks used in attempts to establish self-sustaining natural populations. In addition to monitoring the effects of the individual enhancement programs, the study will provide a broader perspective of the kind of results to be expected from different methods of supplementation. Results thus should be of general use in planning and implementing enhancement programs throughout the Columbia River Basin.


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

CBFWA: Nonwatershed Technical Group Comments Recommendation:
Date:
May 13, 1998
[There are no budget numbers associated with this review.]
Comment:
Comments:

Criteria 1: Technical Criteria - Yes

Criteria 2: Objectives Criteria - Yes: Vague compared to other genetics proposals

Criteria 3: Milestones Criteria - Yes

Criteria 4: Resources Criteria - Yes:


CBFWA Funding Recommendation Recommendation:
Fund
Date:
May 13, 1998
1999 2000 2001
$225,000 $250,000 $250,000
Comment:
The proposed budget reduction proportionally reduces information from this study

ISRP Review , ISRP 1998-1 Recommendation:
Adequate
Date:
Jun 18, 1998
[There are no budget numbers associated with this review.]
Comment:
This is a well-written proposal for needed information. This is the kind of work that should be done before major hatchery projects are implemented or expanded. Methods are thoroughly described and results to date have been published in the open literature. The small sampling of returning adults may limit what information can be gathered.

NW Power and Conservation Council's FY 2006 Project Funding Review Funding category:
expense
Date:
May 2005
FY05 NPCC Start of Year:
$460,500
FY06 NPCC Staff Preliminary:
$460,500
FY06 NPCC July Draft Start of Year:
$460,500
Sponsor (NOAA Fisheries) Comments (Go to Original on NPCC Website):

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