Return to Proposal Finder FY 1999 Proposal 199005200

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date


Section 1. General Administrative Information

Title of Project Proposal Performance/Stock Productivity Impacts of Hatchery Supplementation
BPA Project Proposal Number 199005200
Business name of agency, institution,
or organization requesting funding
Biological Resources Division, U.S. Geological Survey
Business acronym (if appropriate) USGS
 

Proposal contact person or principal investigator

Name Reg Reisenbichler
Mailing Address 6505 NE 65th St.
City, State, Zip Seattle, WA 98115
Phone 2065266282 334
Fax 2065266654
E-mail Reg_Reisenbichler@usgs.gov
 
Manager of program authorizing this project
 
Review Cycle FY 1999
Province Systemwide
Subbasin Systemwide
 
Short Description Provide increased understanding of reputed failure of supplementation w/steelhead in Idaho's Clearwater River & an improved basis for planning/modifying supplementation programs & program evaluations by measuring genetic effects from artificial propagatio
Target Species Anadromous Oncorhynchus spp. (particularly steelhead and spring chinook salmon) throughout the Columbia River basin (and throughout their range).


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


NPPC Program Measure Number(s) which this project addresses:
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: BiOp designation from 4/16/03 budget estimates spreadsheet.
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

n/a or no information


Section 3. Relationships to Other Projects

n/a or no information


Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Compare growth & survival of genetically marked offspring from wild steelhead and Dworshak steelhead reared in 2 natural streams. Comparisons to be completed for each of 4 year-classes. Growth & survival evaluated at end of each season & at migration. a. Sample hatchery fish, capture wild adults. Determine PEPA types, retain only wild fish homozygous for common allele, only hatchery fish homozygous for alternate allele.Combine gametes, incubate, release experimental groups as fry in 20-Mile & Silver creek
1. Compare growth & survival of genetically marked offspring from wild steelhead and Dworshak steelhead reared in 2 natural streams. Comparisons to be completed for each of 4 year-classes. Growth & survival evaluated at end of each season & at migration. b. Sample juveniles in streams at end of each growing season, trap migrating juveniles; determine relative survival & size of offspring. Estimate standing stock of experimental fish in streams & number of outmigrants, & account for differential outmigration.
2. Compare growth & survival of genetically marked offspring from wild chinook from Warm Springs R. & Warm Springs Hatchery in 2 natural streams. Compare for each of 4 year-classes. Growth & survival evaluated at end of each season & at migration. a. Capture hatchery & wild adults, combine gametes to create genetically marked groups; release these experimental groups as eyed embryos or swim-up fry in Little White Salmon River & Buck Creek.
2. Compare growth & survival of genetically marked offspring from wild chinook from Warm Springs R. & Warm Springs Hatchery in 2 natural streams. Compare for each of 4 year-classes. Growth & survival evaluated at end of each season & at migration. b. Sample juveniles residing in the study streams at the end of each growing season, and trap juveniles migrating downstream. Determine relative survival and size of the offspring of hatchery and wild fish.
3. Compare growth & survival of genetically marked offspring from local wild fish & Dworshak, Clearwater or Warm Springs hatcheries raised together. Growth & survival evaluated immediately before release from hatchery as smolts. a. Rear genetically marked groups of fish, spawned in Tasks 1a or 2a, together in hatchery troughs or ponds using standard hatchery rearing practices and release the fish at the standard time of release.
3. Compare growth & survival of genetically marked offspring from local wild fish & Dworshak, Clearwater or Warm Springs hatcheries raised together. Growth & survival evaluated immediately before release from hatchery as smolts. b. Compare the relative survival and size of experimental fish shortly before the standard release date.
3. Compare growth & survival of genetically marked offspring from local wild fish & Dworshak, Clearwater or Warm Springs hatcheries raised together. Growth & survival evaluated immediately before release from hatchery as smolts. c. Mark experimental fish; determine relative adult return rates/sizes. Transport 100 juvenile sp. Chinook to BRD's Marrowstone Field Station for 3-6 mos. Of growth monitoring.
4. Compare reproductive success of genetically marked adult offspring from wild steelhead and hatchery steelhead. Compare for each of 4 year-classes. a. Develop length-fecundity & weight-fecundity relations for hatchery fish & for wild fish spawned for Objs. 1 and 3. Determine whether size of eggs differs between hatchery fish and wild fish.
4. Compare reproductive success of genetically marked adult offspring from wild steelhead and hatchery steelhead. Compare for each of 4 year-classes. b. Capture adult fish returning to Selway R. or adult experimental fish returning to Crooked R. Release radio-tagged adults with appropriate PEPA into Silver Cr. Estimate # of eggs in hatchery females & wild females above barriers.
4. Compare reproductive success of genetically marked adult offspring from wild steelhead and hatchery steelhead. Compare for each of 4 year-classes. c. Sample juveniles and experimental fish released into study sections at end of each growing season. Trap juveniles that migrate downstream, test for differences in migration between groups.
5. Test for selection on genetic marks. The test fish will be the offspring of hatchery fish. The tests will be repeated for up to four year-classes, depending on preliminary results from the first two year-classes. a. At the respective hatcheries, mate adults heterozygous at the locus used for genetic marks (sSOD-1 or PEPA). Release at least 20,000 of the resulting fish (from each year-class) in a study stream, and rear at least 60,000 in the hatchery.
5. Test for selection on genetic marks. The test fish will be the offspring of hatchery fish. The tests will be repeated for up to four year-classes, depending on preliminary results from the first two year-classes. b. Take a sample of juvenile fish from the study stream at the end of each growing season, and determine the mean size and relative survival of fish with the different genotypes.
5. Test for selection on genetic marks. The test fish will be the offspring of hatchery fish. The tests will be repeated for up to four year-classes, depending on preliminary results from the first two year-classes. c. Compare the relative survival and size of experimental fish with the different genotypes immediately before the standard release date at the hatchery.
5. Test for selection on genetic marks. The test fish will be the offspring of hatchery fish. The tests will be repeated for up to four year-classes, depending on preliminary results from the first two year-classes. d. Determine relative return rates and sizes of fish with the different genetic marks when they return to the hatchery as adults.
6. Test for an effect of incubation temperature by comparing the growth and survival of juvenile steelhead from matings early in the season, incubated at 7?C and from matings made later, incubated at 12?C. a. Spawn adult steelhead at Dworshak. Mark otoliths of juveniles thermally. Release half of fish as unfed fry in N.Frk Palouse R; rear remainder in hatchery.
6. Test for an effect of incubation temperature by comparing the growth and survival of juvenile steelhead from matings early in the season, incubated at 7?C and from matings made later, incubated at 12?C. b. Take a sample of juvenile fish from the study stream at the end of each growing season, and determine the mean size and relative abundance of fish from the two treatments (early, 7?C vs. late, 12?C).
6. Test for an effect of incubation temperature by comparing the growth and survival of juvenile steelhead from matings early in the season, incubated at 7?C and from matings made later, incubated at 12?C. c. Compare the relative survival and size of early and late experimental fish immediately before the standard release date at the hatchery.
6. Test for an effect of incubation temperature by comparing the growth and survival of juvenile steelhead from matings early in the season, incubated at 7?C and from matings made later, incubated at 12?C. d. Determine relative return rates and sizes of fish with the different genetic marks when they return to the hatchery as adults.
7. Test for effect of cryopreservation via growth & mortality comparison & response of juveniles to other stressors, using "fresh" & "cryo" milt. Challenge with 1) pathogen, 2) restricted ration, 3) acute handling, 4) 48-hr density stress, 5) saltwater. a. Take milt from 12 hatchery males, divide into "cryo" and "fresh" portions; take eggs from 12 hatchery females, divide into equal portions. Fertilize one portion with "cryo" milt, one with "fresh." Use different male with each female.
7. Test for effect of cryopreservation via growth & mortality comparison & response of juveniles to other stressors, using "fresh" & "cryo" milt. Challenge with 1) pathogen, 2) restricted ration, 3) acute handling, 4) 48-hr density stress, 5) saltwater. b. Incubate each group separately; divide each family; mark with mirror fin clips. One group will receive high ration levels; the other fed at 1/2 recommended level. Remaining fish reared separately by treatment at high level ration.
7. Test for effect of cryopreservation via growth & mortality comparison & response of juveniles to other stressors, using "fresh" & "cryo" milt. Challenge with 1) pathogen, 2) restricted ration, 3) acute handling, 4) 48-hr density stress, 5) saltwater. c. Monitor growth & survival, test for differences between treatments.

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 06/01/91 12/01/99 25.0%
2 06/01/91 12/01/01 15.0%
3 06/01/91 12/01/02 20.0%
4 01/01/92 12/01/02 12.0%
5 06/01/91 07/01/02 20.0%
6 01/01/94 06/01/00 5.0%
7 01/01/96 12/01/98 3.0%


Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 1999 Cost
Personnel $157,973
Fringe $ 45,022
Supplies $ 48,756
Operating $ 11,832
Travel $ 18,312
Indirect $111,615
Subcontractor $102,983
Total Itemized Budget $496,493


Total estimated budget

Total FY 1999 project cost $496,493
Amount anticipated from previously committed BPA Funds $ 0
Total FY 1999 budget request $496,493
FY 1999 forecast from 1998 $ 0
% change from forecast 0.0%


Reason for change in estimated budget

Not applicable


Reason for change in scope

Not applicable


Cost Sharing

Not applicable
 

Outyear Budget Totals

2000 2001 2002
All Phases $430,000 $335,000 $315,000
Total Outyear Budgets $430,000 $335,000 $315,000
 

Other Budget Explanation

Schedule Constraints: Identify any constraints that may cause schedule changes. Describe major milestones if necessary. Arbitrary budget reductions, such as the arbitrary reduction of our budget for 1998 by $41,000, will postpone or preclude completion of objectives because we will have inadequate manpower to accomplish the field and lab work and report preparation.


Section 6. References

n/a or no information


Section 7. Abstract

Abstract

The methods involve crossing adult fish to produce genetically marked progeny of hatchery x hatchery, hatchery x wild, and wild x wild parentage. The progeny are incubated in identical conditions in the hatchery until button-up when the groups are mixed together. Mixed populations are divided; some fish are released into natural streams, others are reared in the hatchery. The progeny are sampled before or during their downstream migration or as returning adults to determine relative growth and survival of the treatment groups. The relative performances will be used to infer effects on stock productivity from using progeny of wild fish in the hatchery and hatchery fish in streams, and to design evaluations. The study will be completed in December 2002.


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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