Return to Proposal Finder FY 1999 Proposal 199009300

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Life History and Genetic Analysis of Oncorhynchus nerka
BPA Project Proposal Number 199009300
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI

Proposal contact person or principal investigator

Name Madison S. Powell
Mailing Address 3059 F National Fish Hatchery Road
City, State, Zip Hagerman, ID 83332
Phone 2088379096
Fax 2088376047
Manager of program authorizing this project
Review Cycle FY 1999
Province Mainstem
Subbasin Mainstem
Short Description Provide biological and genetic information on O. nerka samples collected throughout the Snake and Columbia River Basins to be used in the overall recovery effort of the Snake River sockeye salmon.
Target Species Snake River sockeye salmon (ESA)

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 7.5A.1
FWS/NMFS Biological Opinion Number(s) which this project addresses: NMFS-Hydrosystems Operations Biological Opinion, Endangered Species Act requirements
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: BiOp designation from 4/16/03 budget estimates spreadsheet.
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
9107100 Snake River Sockeye Salmon Habitat (Sho-Ban Tribes) This project provides genetic information for habitat and resource management. The 9107100 project provides tissue samples for this project. No
9107200 Redfish sockeye Salmon Captive Broodstock (IDFG) This project provides genetic information on an endangered population. The 9107200 project provides tissue samples for this project. No
9204000 Redfish Lake Sockeye Salmon Captive Broodstock Rearing and Research (NMFS) This project provides genetic information on an endangered population.The 9204000 project provides tissue samples for this project. No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Genetic analysis of outmigrant assemblages . Continue DNA analyses on tissue samples from outmigrant assemblages (from Redfish, Alturas, and Pettit Lakes) to assess contributions from kokanee and resident sockeye populations on subsequent year classes.
2. Genetic analysis of Redfish Lake creel surveys . Continue screening tissue samples from Redfish Lake creel surveys to assess potential harvest of listed, resident sockeye and to establish baseline information on the genetic diversity of kokanee harvested.
3. Expansion of present mitochondrial database . Continue to expand the present mitochondrial DNA RFLP database for Redfish Lake and other Columbia River Basin O. nerka populations.
4. Analysis of captive broodstock progeny . Continue DNA analyses on tissue samples of captive broodstock progeny for comparison to returning sockeye assemblages and thus, characterize successful outmigrant contributions.
5. Evaluation of nuclear DNA markers . Continue to evaluate nuclear DNA markers for their utility in distinguishing populations of O. nerka.
6. Genetic evaluation of stray O. nerka . Continue to examine various tissue samples of interest to evaluate the origin of stray O. nerka in the Columbia River Basin.
7. Genetic analysis of early and late spawning O. nerka . Continue the analysis of genetic differences and population substructure among early and late spawning O. nerka.

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 01/01/99 12/01/01 15.0%
2 01/01/99 12/01/01 15.0%
3 01/01/99 12/01/99 15.0%
4 01/01/99 12/01/99 15.0%
5 01/01/99 12/01/01 25.0%
6 01/01/99 12/01/01 10.0%
7 01/01/99 12/01/99 5.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 1999 Cost
Personnel 1. M. Powell, Research Scientist 2. Senior Scientific Aide 3. Laboratory Technician $ 64,594
Fringe @ .282 for #1 and #2, @.345 for #3 $ 19,117
Supplies chemicals, pipet tips, tubes, gloves etc. $ 8,000
Operating equipment service, calibration, UPS shipping, Federal Express, long distance phone charges $ 2,000
Capital none $ 0
Tag none $ 0
Travel 1 professional meeting, 12 monthly T.O.C. meetings/1 person. $ 2,650
Indirect on campus cost @ .447% rate $ 43,073
Subcontractor $ 0
Total Itemized Budget $139,434

Total estimated budget

Total FY 1999 project cost $139,434
Amount anticipated from previously committed BPA Funds $ 0
Total FY 1999 budget request $139,434
FY 1999 forecast from 1998 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2000 2001
All Phases $ 70,000 $ 70,000
Total Outyear Budgets $ 70,000 $ 70,000

Other Budget Explanation

Schedule Constraints: Schedule changes may depend on returning adult Redfish Lake sockeye. Mitochondrial DNA database (#3), captive broodstock analysis (#4), and early/late spawning O. nerka (#7) objectives will be completed in 1999.

Section 6. References

n/a or no information

Section 7. Abstract


This ongoing project seeks to: (a) comprehensively identify the genetic structure of Redfish Lake O. nerka outmigrant-originating populations; (b) provide long term information about the genetic identity of returning anadromous sockeye as this run is restored; (c) define the relatedness of populations of O. nerka in Redfish, Stanley, and Snake River lakes and the Columbia Basin lakes; (d) provide information to monitor the change or loss of genetic biodiversity among O. nerka populations throughout the Columbia Basin and in particular, Redfish Lake, Idaho. Thus far, a total of 1373 tissue samples from 39 populations have been characterized using mitochondrial DNA analyses. Additionally, several nuclear DNA sequences have also been examined for their utility in delineating Oncorhynchus nerka stocks. Presently available genetic markers have been satisfactory in separating beach spawning, resident sockeye from Fishhook Creek kokanee. DNA analyses have also provided evidence demonstrating Fishhook Creek kokanee appear to contribute little if any to the O. nerka outmigrants from Redfish Lake. Outmigrant assemblages appear to be primarily composed of progeny from resident sockeye and captively bred smolts released into the lake. Mitochondrial and nuclear DNA analyses have provided additional evidence that beach spawning, resident sockeye are most closely related to anadromous sockeye. Fishhook Creek kokanee are genetically diverse and during spawning are temporally and spatially isolated from the resident sockeye population. A total of 35 composite mitochondrial haplotypes have been observed from populations of O. nerka sampled throughout the Columbia River Basin. Mitochondrial genetic data from 1996 and 1997 Redfish Lake creel samples putatively indicate the incidental take of a listed resident sockeye. Most recently, mitochondrial genetic data were used to demonstrate stray sockeye returning to the NMFS Big Beef Creek facility were not of Redfish Lake origin and unlikely to be of Lake Wenatchee origin as well.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

CBFWA: Nonwatershed Technical Group Comments Recommendation:
May 13, 1998
[There are no budget numbers associated with this review.]

Criteria 1: Technical Criteria - Yes

Criteria 2: Objectives Criteria - Yes

Criteria 3: Milestones Criteria - Yes

Criteria 4: Resources Criteria - Yes:

CBFWA Funding Recommendation Recommendation:
May 13, 1998
1999 2000 2001
$139,434 $ 70,000 $ 70,000

ISRP Review , ISRP 1998-1 Recommendation:
Jun 18, 1998
[There are no budget numbers associated with this review.]
This is a well-written proposal for work that is very important to the program because it traces the genetic effects of hatchery fish on wild fish.

NW Power and Conservation Council's FY 2006 Project Funding Review Funding category:
May 2005
FY05 NPCC Start of Year:
FY06 NPCC Staff Preliminary:
$ 98,000
FY06 NPCC July Draft Start of Year:
$ 98,000
Sponsor (U of Idaho) Comments (Go to Original on NPCC Website):

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