Return to Proposal Finder FY 2000 Proposal 20044

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Endocrine Control of Ovarian Development in Salmonids
BPA Project Proposal Number 20044
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI

Proposal contact person or principal investigator

Name Dr. James J. Nagler
Mailing Address
City, State, Zip Moscow, ID 83844-3051
Phone 2088854382
Fax 2088857905
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Study key intra-ovarian endocrine pathways in salmonids as a means to address reproductive problems in captive broodstock programs. Provide basis for technological development to diagnose sub-fertility and increase embryo viability.
Target Species Oncorhynchus mykiss (steelhead/rainbow trout)

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 7.2; 7.4D
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
Intracytoplasmic sperm injection: genetic retrieval from single sperm Fish Reproduction Program participant No
Analyzing genetic and behavioral changes during salmonid domestication Fish Reproduction Program participant No
Induction of precocious sexual maturity and enhanced egg production in fish Fish Reproduction Program participant No
Enhancement of salmonid gamete quality by manipulation of intracellular ATP Fish Reproduction Program participant; co-investigator No
Viral vaccines and effects on reproductive status Fish Reproduction Program participant No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. determine an intra-ovarian endocrine role for estradiol a. set up and optimize methods to quantify salmonid IGF mRNA and protein
1. b. test whether estradiol can influence IGF mRNA and/or protein levels within the ovary
2. promote ovarian follicle growth a. test whether estradiol, or other endocrine factors produced within the ovary, can increase ovarian growth
3. influence the transcription of oocyte vitellogenin receptors a. develop suitable oocyte vitellogenin receptor mRNA probes
3. b. test the ability of intra-ovarian endocrine factors (i.e. estradiol, IGFs) to modulate vitellogenin receptor mRNAs

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 10/01/99 10/01/00 experimental data showing the effects of estradiol on in vitro IGF mRNA and/or protein synthesis by the salmonid ovary 33.0%
2 10/01/99 10/01/00 hormonal enhancement of ovarian growth 33.0%
3 10/01/99 10/01/00 upregulation/ downregulation of oocyte vitellogenin receptor transcripts by the intra-ovarian endocrine agents tested 33.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel salaries: PI (2 mo); research technician and postdoctoral fellow (12 mo) $ 73,560
Fringe 28.5% for PI and professional staff $ 20,965
Supplies consumables, reagents, plumbing supplies, fish $ 13,000
Operating equipment repair and maintenance $ 5,000
Capital water chillers, recirculating pumps, experimental tanks $ 10,000
Travel annual professional meeting $ 2,000
Indirect UI rate of 44.7% on total direct costs $ 68,625
Other aquaculture core facility; hormone core facility; administrative core facility $ 29,000
Total Itemized Budget $222,150

Total estimated budget

Total FY 2000 project cost $222,150
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $222,150
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2001 2002 2003 2004
All Phases $201,700 $209,033 $216,732 $224,815
Total Outyear Budgets $201,700 $209,033 $216,732 $224,815

Other Budget Explanation

Schedule Constraints: None

Section 6. References

Reference Watershed?
Barr, W.A. 1963. The endocrine control of the sexual cycle in the plaice, Pleuronectes platessa (L). II. The endocrine control of oogenesis. Gen. Comp. Endocrinol. 3: 205-215. No
Blaxter, J.H.S., 1988. Pattern and variety in development. In: Fish Physiology, (Hoar and Randall, eds.) Vol. XI, The Physiology of developing fish, Part A, Eggs and Larvae. Academic, San Diego, pp. 1- 58. No
Cao, Q.-P., S.J. Duguay, E. Plisetskaya, D.F. Steiner and S.J. Chan. 1989. Nucleotide sequence and growth hormone-regulated expression of salmon insulin-like growth factor I mRNA. Mol. Endocrinol. 3: 2005-2010. No
Chen, T.T., A. Marsh, M. Shamblott, K.-M. Chan, Y.-L. Tang, C.M. Cheng and B.-Y. Yang. 1994. Structure and evolution of fish growth hormone and insulinlike growth ... In: Fish Physiology, (Hoar and Randall, eds.) Vol. XIII, pp.179-203. Academic Press, NY. No
Das, S.K., H. Tsukamura, B.C. Paria, G.K. Andrews and S.K. Dey.1994. Differential expression of epidermal growth factor receptor (EGR-R) gene and regulation of EGF-R bioactivity by progesterone and estrogen in the adult mouse … Endocrinology 134:971-981. No
Davidson, E.H. 1986. Gene activity in early development. Academic Press, Inc. Orlando, FL. No
Dorrington, J. H., J.J. Bendell, and S.A. Khan. 1993. Interactions between FSH, estradiol-17-beta and transforming growth factor-beta regulate growth and differentiation in the rat gonad. J. Steroid Biochem. Mol. Biol. 44: 441- 449. No
Duguay, S.J., L.K. Park, M. Samadpour, and W.W. Dickhoff. 1992. Nucleotide sequence and tissue distribution of three insulin-like growth factor I prohormones in salmon. Mol. Endocrinol. 6: 1202-1210. No
Hsu, C.-J. and J.M. Hammond. 1987. Gonadotropins and estradiol stimulate immunoreactive insulin-like growth factor-I production by porcine granulosa cells in vitro. Endocrinology 120: 198-207. No
Kagami, I., H. Mizunuma, S. Miyamoto, Y. Ibuki, and T. Uchida. 1996. Quantification of estrogen receptor messenger RNA by quantitative polymerase chain reaction using internal standard fragment. Biochem. Biophys. Res. Com. 228: 358-364. No
Maestro, M.A., E. Mendez, M. Parrizas, and J. Gutierrez. 1997. Characterization of insulin and insulin-like growth factor-1 ovarian receptors during the reproductive cycle of carp (Cyprinus carpio). Biol. Reprod. 56: 1126-1132. No
Manova, K., E.J. Huang, M. Angeles, V. De Leon, S. Sanchez, S.M. Pronovost, P. Besmer, and R.F. Bachvarova. 1993. The expression pattern of the c-kit ligand in gonads of mice supports a role for the c-kit receptor in oocyte … Dev. Biol. 157: 85-99. No
Nagahama, Y., M. Yoshikuni, M. Yamashita, and M. Tanaka. 1994. Regulation of oocyte maturation in fish. In: Fish Physiology, (Hoar, Randall, and Farrell, eds.) Vol. XIII, pp. 331-357. Academic Press, New York. No
Nagler, J.J., A.P. Scott, C.R.Tyler and J.P. Sumpter. 1996. Gonadotropins I and II do not stimulate the in vitro secretion of 17a,20b-dihydroxy-4-pregnen-3-one-20-sulphate by rainbow trout gonads during final sexual … Fish Physiol. Biochem.15: 149-156. No
Nagler, J.J., C.R. Tyler and J.P. Sumpter. 1994. Ovarian follicles of rainbow trout (Oncorhynchus mykiss) cultured within lamellae survive well, and sequester and process vitellogenin. J. Exp. Zool. 269:45-52. No
Nagler, J.J., and D.G. Cyr. 1997. Exposure of male American plaice (Hippoglossoides platessoides) to contaminated marine sediments decreases the hatching success of their progeny. Environ. Tox. Chem. 16: 1733-1738. No
Nagler, J. J. and D.G. Cyr. 1998. Different gonadal estrogen receptor messenger RNAs in the rainbow trout: is this ER-a and ER-b? Biol. Reprod. 58: Suppl. 1, Abstract No. 232. No
Negatu, Z., S.M. Hsiao, and R.A.Wallace. 1998. Effects of insulin-like growth factor-I on final oocyte maturation and steroid production in Fundulus heteroclitus. Fish Physiol. Biochem. 19:13-21. No
Packer, A.I., Y. Hsu, P. Besmer, and R.F. Bachvarova. 1994. The ligand of the c-kit receptor promotes oocyte growth. Dev. Biol. 161: 194-205. No
Prat, F., J.P. Sumpter, C.R. Tyler. 1996. Validation of radioimmunoassays for two salmon gonadotropins (GtH I and GtH II) and their plasma concentrations throughout the reproductive cycle in male and female rainbow trout. Biol. Reprod. 54: 1375-1382. No
Prat, F., K. Coward, J.P. Sumpter, and C.R. Tyler. 1998. Molecular characterization and expression of two ovarian lipoprotein receptors in the rianbow trout (Oncorhynchus mykiss). Biol. Reprod. 54: 1375-1382. No
Pratt, W.B. and L.C. Scherrer. 1993. Heat shock proteins and the cytoplasmic-nuclear trafficking of steroid receptors. In Steroid Hormone Receptors: Basic and Clinical Aspects, V.K. Moudgil, Ed., Birkhauser Boston, pp. 215- 246. No
Richards, J. 1994. Hormonal control of gene expression in the ovary. Endocrine Rev. 15:725-751. No
Shamblott, M.J. and T.T. Chen. 1992. Identification of a second insulin-like growth factor in a fish species. Proc. Natl. Acad. Sci. USA 89:8913-8917. No
Suzuki, K., Y. Nagahama, and H. Kawauchi. 1988. Steroidogenic activities of two distinct salmon gonadotropins. Gen. Comp. Endocrinol. 71: 452-458. No
Swanson, P., T. Flagg, J. Hard, L. Harrell, K. Shearer, R. Pascho, W. Hershberger, K. Massey, and R. Hardy. 1995. Research on captive broodstock technology for Pacific salmon. Annual Report 1995. DE-AI79-93BP55064, BPA, Portland, OR. No
Tyler, C.R. and P. Lancaster. 1993. Isolation and characterizaton of the receptor for vitellogenin from follicles of the rainbow trout, Oncorhynchus mykiss. J. Comp. Physiol. B 163: 225-233. No
Tyler, C.R., J. J. Nagler, T. G. Pottinger and M. A. Turner. 1994. Effects of unilateral ovariectomy on recruitment and growth of follicles in the rainbow trout, Oncorhynchus mykiss. Fish Physiol. Biochem. 13:309-316. No
Tyler, C.R., T.G. Pottinger, K. Coward, F. Prat, N. Beresford, and S. Maddix. 1997. Salmonid follicle-stimulating hormone (GTH I) mediates vitellogenic development of oocytes in the rainbow trout, Oncorhynchus mykiss. Biol. Reprod. 57:1238-1244. No
Yan, L., P. Swanson and W.W. Dickhoff. 1992. A two-receptor model for salmon gonadotropins (GTH I and GTH II). Biol. Reprod. 47:418-427. No
Wallace, R.A. 1985. Vitellogenesis and oocyte growth in nonmammalian vertebrates. In: L.W. Browder (Ed) Developmental biology, Vol.1., Plenum Press, New York, pp. 127-177. No
Zafarullah, M., J. Wisniewski, N.W. Shworak, S. Schieman, S. Misra and L. Gedamu. 1992. Molecular cloning and characterization of a constitutively expressed heat-shock-cognate hsc7gene from rainbow trout. Eur. J. Biochem. 204: 893-900. No

Section 7. Abstract


Reduced female reproductive performance is currently limiting the number of viable offspring produced by captive salmonid broodstock programs in the Pacific Northwest. The consequences of these difficulties is an uncertain supply of quality eggs for artificial propagation, and endangerment of certain populations where the number of remaining females is small. An explanation for some instances of sub-fertility and variable embryo survival can undoubtedly be linked to oogenesis and the endocrine events that regulate oocyte development. To date, our understanding of intra-ovarian endocrine physiology in salmonids is limited, but research in this area could make an important contribution toward improving reproductive success. The discovery, in my laboratory, of estrogen receptor transcripts in the rainbow trout (Oncorhynchus mykiss) ovary raises the possibility that estrogens have important effects within the ovary that relate directly to oocyte development. The general hypothesis of this proposal is that an understanding of key endocrine processes within the developing salmonid ovary, particularly those involving estradiol (E2), will lead to ways of diagnosing sub-fertility and improving reproductive success. The specific objectives are to, 1) establish whether the principal female sex steroid, E2, can stimulate insulin-like growth factor production in the ovary, 2) determine whether E2, or hormones stimulated by E2, can promote the growth and development of ovarian follicles, and 3) study whether E2 can influence the transcription of genes for vitellogenin receptors within developing oocytes. The outcome of these studies are two-fold, first, it will generate basic information to understand what controls the normal growth of ovarian follicles, and secondly, to provide a basis for future strategies and technologies to improve salmonid fecundity and embryo viability.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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