Return to Proposal Finder FY 2000 Proposal 20045

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date


Section 1. General Administrative Information

Title of Project Proposal Analyzing Genetic and Behavioral Changes During Salmonid Domestication
BPA Project Proposal Number 20045
Business name of agency, institution,
or organization requesting funding
Washington State University
Business acronym (if appropriate) WSU
 

Proposal contact person or principal investigator

Name Gary H. Thorgaard
Mailing Address Department of Zoology
City, State, Zip Pullman, WA 99164-4236
Phone 5093357438
Fax 5093353184
E-mail thorglab@wsu.edu
 
Manager of program authorizing this project
 
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
 
Short Description Analyze genetic changes occurring during domestication in chinook salmon and steelhead trout by studying selection on mapped DNA markers under wild and hatchery conditions and analyze behavioral and physiological changes using standardized tests.
Target Species Chinook salmon (Oncorhynchus tshawytscha), Steelhead trout (Oncorhynchus mykiss)


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


NPPC Program Measure Number(s) which this project addresses: 7.1.F.2, 7.2.A.1, 7.4.D.1
FWS/NMFS Biological Opinion Number(s) which this project addresses: -
Other Planning Document References Technical Recommendations 4 and 5 from Executive Summary, “Spirit of the Salmon”


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

n/a or no information


Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
Intracytoplasmic Sperm Injection: Genetic Retrieval from Sperm also part of UI/ WSU Fish Reproduction program grant No
Endocrine Control of Ovarian Development in Salmonids also part of UI/ WSU Fish Reproduction program grant No
Induction of Precocious Sexual Maturity and Enhanced Egg Production in Fish also part of UI/ WSU Fish Reproduction program grant No
Motility and Fertility of Salmonid Gametes also part of UI/ WSU Fish Reproduction program grant No
Viral Vaccines and Effects on Reproductive Status also part of UI/ WSU Fish Reproduction program grant No


Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Develop a genetic map of microsatellite markers for chinook salmon. a. Test primers from other salmonid species for amplification in chinook salmon
1. b. Prepare mapping cross for chinook salmon
1. c. Identify markers that are polymorphic in mapping cross
1. d. Map markers to develop chinook salmon map
2. Conduct tests for three behavior patterns in strains of chinook salmon and steelhead trout with varying histories of domestication which have been raised in a common environment from the egg stage. a. Test for differences in use of water column
2. b. Test for differences in startle response
2. c. Test for differences in aggressive interactions
3. Investigate physiological responses to acute and sudden environmental stressors in the chinook salmon and steelhead trout with varying histories of domestication. a. Test for differences in cortisol levels related to startle response
3. b. Test for differences in cortisol levels related to handling and restraint
3. c. Test for variation in cortisol level in eggs

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 10/01/99 09/01/02 Develop chinook salmon genetic map 200 marker map 44.8%
2 10/01/99 09/01/04 Identify behavior differences related to domestication Behavioral assay for domestication 28.7%
3 10/01/99 09/01/04 Identify stress response differences related to domestication Cortisol assay for domestication 26.5%
4 10/01/99 09/01/04 Correlation of DNA markers with survival in hatchery and wild environments Possible major gene effects identified related to domestication  
5 10/01/99 09/01/04 Measure levels of selection in hatchery and wild environments Information on selection levels in hatchery & wild environments  


Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel .5 mo salary (summer) for 3 co-Pis; 2 postdoc fellows (1 yr), 1 sem. Support for grad research asst $ 70,924
Fringe Benefits for the above six employees $ 20,874
Supplies Molecular biology supplies for mapping studies ($15K), behavior testing ($3K), hormone testing ($6K) $ 24,000
Capital PCR machine for genetic mapping studies ($4,000), Video equipment for behavior studies ($10,000) $ 14,000
Travel Travel to collect gametes and attending a scientific meeting $ 1,000
Indirect 45% of direct costs excluding equipment and student tuition $ 59,922
Other Costs of aquaculture core lab ($15K) and admin core for Fish Reproduction Program ($4K) $ 19,000
Total Itemized Budget $209,720


Total estimated budget

Total FY 2000 project cost $209,720
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $209,720
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%


Reason for change in estimated budget

Not applicable


Reason for change in scope

Not applicable


Cost Sharing

Not applicable
 

Outyear Budget Totals

2001 2002 2003 2004
All Phases $203,549 $211,741 $220,120 $229,120
Total Outyear Budgets $203,549 $211,741 $220,120 $229,120
 

Other Budget Explanation

Not applicable


Section 6. References

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Kocher, T.D., W-J Lee, H. Sobolewska, D. Penman and B. McAndrew. 1998. A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus ). Genetics 148: 1225-1232. No
Kohane, M.J. and P.A. Parsons. 1989. Domestication: evolutionary changes under stress. Evol. Biol. 23:31-48. No
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Section 7. Abstract

Abstract

A serious concern in regional salmon and steelhead management programs is that hatchery rearing may select genetically for fish with reduced viability in the wild. It is important to understand the types of genetic and behavioral changes that may be occurring during the domestication process. We propose to investigate domestication-related genetic and behavioral changes using controlled crosses of strains of chinook salmon and steelhead trout with varying degrees of hatchery ancestry. A genetic map of microsatellite markers will be developed for chinook salmon and this map, together with an already-developed map for steelhead, will be used in crosses that test for DNA markers associated with the ability of chinook salmon and steelhead trout to survive in hatchery or wild environments. We will also measure the level of selection occurring in populations of chinook salmon and steelhead trout in hatchery and wild environments by measuring the levels of fluctuating asymmetry of the same populations reared in these environments. Behavior patterns and physiological responses to acute and sudden environmental stressors of wild and hatchery strains of both species which have been raised in a common environment from the egg stage will be characterized. On completion of this study, we will have initiated the characterization of the types of genetic changes associated with domestication in chinook salmon and steelhead trout, and will have identified standard behavioral and physiological tests that can be used to monitor levels of domestication in these species.


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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