Return to Proposal Finder FY 2000 Proposal 20046

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Induction of Precocious Sexual Maturity and Enhanced Egg Production in Fish
BPA Project Proposal Number 20046
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI

Proposal contact person or principal investigator

Name Gerald T. Schelling
Mailing Address Dept. of Animal & Veterinary Science, U of Idaho
City, State, Zip Moscow, ID 838344
Phone 2088857310
Fax 2088856420
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Determine if female fish sexual maturity can be achieved earlier (thus increased reproductive capacity and reduced generation time) due to a drastic growth rate increase (3.5 times normal) induced by the use of somatotropin.
Target Species Salmonid, White Sturgeon

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 7.2, 7.4D (1994 Columbia River Basin Fish and Wildlife Program)
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
Intracytoplasmic Sperm Injection: Genetic Retrieval from Single Sperm Project participant No
Endocrine Control of Ovarian Development in Salmonids Project participant No
Analyzing Genetic and Behavioral Changes During Salmonid Domestication Project participant No
Enhancement of Salmonid Gamete Quality by Manipulation of Intracellular ATP Project participant No
Viral Vaccines and Effects on Reproductive Status Project participant No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Test the effects of various dosage levels of bovine somatotropin on physiological growth in young white sturgeon. a. Conduct growth trials with 40,80 and 120 mcg somatotropin/g body weight to determine optimal growth conditions.
2. Test the effects of various dosage levels of bovine somatotropin on physiological growth and its relationship to a) the timing of sexual maturity, b) fecundity and c) egg quality in rainbow trout. b. Conduct trout trials to determine optimal growth conditions and carry out the sexual maturity, fecundity and egg quality studies.
3. Investigate the relationship of growth hormone, IGF-I and IGF-II to growth rate and sexual maturity in rainbow trout and white sturgeon. c. Conduct trout trials to determine optimal growth conditions and carry out the sexual maturity, fecundity and egg quality studies.
4. Test the effects of various dosage levels of bovine somatotropin on physiological growth and its relationship to a) the timing of sexual maturity, b) fecundity and c) egg quality in white sturgeon. d. Conduct the necessary animal trials with graded levels of somatotropin, other than that optimal for growth to determine the effect of these levels on sexual maturity fecundity and egg quality.
5. Test the effects of various dosage levels of bovine somatotropin on the periodicity of white sturgeon spawning. e. Carry out the trials to determine the periodicity of spawning.

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 11/01/99 02/01/00 Optimal growth conditions 9.0%
2 11/01/99 04/01/01 Determination of earlier sexual maturity in salmonids if successful Yes 25.0%
3 06/01/00 02/01/02 An understanding of the growth stimulating hormones and onset of sexual maturity 19.0%
4 01/01/02 10/01/03 Determination of earlier sexual maturity in sturgeon if successful Yes 28.0%
5 01/01/02 09/01/04 Determination of periodicity of sturgeon spawning Yes 19.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel Post-docotral research assoc. $ 33,000
Fringe Post-docotral research assoc. $ 9,405
Supplies $ 34,450
PIT tags 200 $ 580
Travel One meeting. Travel to off-campus fish laboratory $ 3,900
Indirect 31.5% $ 47,145
Other Technical res. Assoc. (100%), fringe, tech. Res. Assoc (34.5%), aquaculture, hormone & admin core $ 68,332
Total Itemized Budget $196,812

Total estimated budget

Total FY 2000 project cost $196,812
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $196,812
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2001 2002 2003 2004
All Phases $191,400 $189,000 $179,500 $162,500
Total Outyear Budgets $191,400 $189,000 $179,500 $162,500

Other Budget Explanation

Not applicable

Section 6. References

Reference Watershed?
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Section 7. Abstract


The overall objective is to develop a bovine somatotropin (bST) treatment regime that will result in the increased reproductive capacity and reduced generation time of female salmonid (rainbow trout) and white sturgeon. We hypothesize that increased growth rate is highly correlated with earlier sexual maturity, as is the case with mammals, and have already demonstrated that the growth of white sturgeon can be increased to 3.5 fold with bST. The general nature of the work will be to induce an accelerated growth rate (of several magnitudes) through the use of sustained-release bST and determine if sexual maturity can be achieved much earlier. Our goal is to develop this application for enhanced fish production through increased reproductive capacity and reduced generation time, as well as elucidate the hormonal relationships underlying the association between growth and sexual maturity in fish. We will work with two different species of Pacific Northwest fish that are representative of diverse physiological traits. White sturgeon will be studied due to their slow growth and delayed timing of sexual maturity, and rainbow trout will serve as a representative of the faster-growing cold water salmonids. Sexual maturity will be determined, egg quantity and quality measurements will be made, and related hormone levels will be studied. This work can be achieved in a reasonable time with rainbow trout, and an assay model will be developed utilizing juvenile, nearly mature, and sexually mature female sturgeon in order to facilitate the timely generation of data necessary for this slower growing species.

Reviews and Recommendations

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