Return to Proposal Finder FY 2000 Proposal 20047

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Enhancement of salmonid gamete quality by manipulation of intracellular ATP
BPA Project Proposal Number 20047
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI

Proposal contact person or principal investigator

Name Rolf L. Ingermann
Mailing Address Department of Biological Sciences
City, State, Zip Moscow, ID 83844-3051
Phone 2088856280
Fax 2088857905
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Develop methods to enhance the short-term storage of unfrozen salmonid gametes and improve the quality of salmonid eggs and nonactivated, activated, and cryopreserved sperm.
Target Species Oncorhynchus tshawytscha (chinook) and Oncorhynchus mykiss (steelhead/rainbow)

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 1994 Columbia River Basin Fish and Wildlife Program: 7.2, 7.4D, 7.4E
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
Intracytoplasmic Sperm Injection: Genetic Retrieval from Single Sperm (Dr. Project Participant No
Endocrine Control of Ovarian Development in Salmonids (Dr. James J. Nagler) Project Participant No
Analyzing Genetic and Behavioral Changes During Salmonid Domestication (Dr. Project Participant No
Induction of Precocious Sexual Maturity and Enhanced Egg Production in Fish Project Participant No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

n/a or no information

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 Correlate ATP content and sperm motility and fertility for sperm from different males  
1 Evaluate whether sperm ATP and/or PCr content can be increased by manipulations of short-term storage conditions a  
1 Examine whether such maintenance conditions will lead to elevated ATP stores in untreated as well as activated sperm Examine whether such maintenance conditions will lead to elevated ATP stores in untreated as well as activated sperm  
1 Examine whether such maintenance conditions will lead to elevated ATP stores in untreated as well as activated sperm Examine whether such maintenance conditions will lead to elevated ATP stores in untreated as well as activated sperm  

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel Rolf Ingermann (3.0 mo); Joseph Cloud (1.0 mo), (James Nagler after FY2000), 1 Technician (12.0 mo), $ 76,000
Fringe RLI, JGC, Tech: 28.5%, GS: 1%, UGS: 1% during school year, 9% during summer $ 16,410
Supplies Supplies (7,000), NMR & Flow Cytometer use (6,000) $ 13,000
Operating Graduate Student Tuition & Fees (2,500), Vehicle Rental (1,000), Publication Costs, Xeroxing, Graphi $ 8,000
Capital Equipment: Microcentrifuge (2,500), Laboratory Computer (2,500) $ 5,000
Travel $ 3,000
Indirect 44.7% $ 56,505
Other Center for Reproductive Biology Administrative Core (5,000) $ 5,000
Total Itemized Budget $182,915

Total estimated budget

Total FY 2000 project cost $182,915
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $182,915
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2001 2002 2003 2004
All Phases $193,761 $203,450 $213,622 $224,304
Total Outyear Budgets $193,761 $203,450 $213,622 $224,304

Other Budget Explanation

Schedule Constraints: None

Section 6. References

Reference Watershed?
Ballard WW. 1973. Normal embryonic stages for salmonid fishes, based on Salmo gairdneri Richardson and Salvelinus fontinalis (Mitchill). J Exp Zool, 184:7-26. No
Benau J, Terner C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3:247-257. No
Bencic DC, Yates TJ, Ingermann RL. 1997. Ecto-ATPase activity of vertebrate blood cells. Physiol Zool 70: 621-630. No
Bencic DC, Krisfalusi M, Cloud JG, Ingermann RL. In Press. ATP levels of chinook salmon (Oncorhynchus tshawytscha) sperm following in vitro exposure to various oxygen tensions. Fish Physiol Biochem. No
Bencic DC, Krisfalusi M, Cloud JG, Ingermann RL. Submitted. Short-term storage of salmonid sperm in air versus oxygen. Prog. Fish Culturist. No
Bhattacharyya A, Pakrashi A. 1993. Specificity of ATP for the initiation of flagellar motility of hamster sperm. Arch Androl, 31:159-165. No
Billard R, Petit J, Jalabert B, Szollosi D. 1974. Artificial insemination in trout using a sperm diluent. In: The Early Life History of Fish. Pp. 715-723. Ed. Blaxter JHS. Springer-Verlag, New York. No
Boulekbache H, Bastin J, Andriamihaja M, Lefebvre B, Joly C. 1989. Ageing of fish oocytes: effects on adenylic nucleotides content, energy charge and viability of carp embryo. Comp Biochem Physiol, 93B: 471-476. No
Brooks S, Tyler CR, Sumpter JP. 1997. Egg quality in fish: what makes a good egg? Rev Fish Biol Fisheries, 7: 387-416. No
Calamera JC, Brugo S, Vilar O. 1982. Relationship between motility and adenosine triphosphate (ATP) in human spermatozoa. Andrologia, 14:239-241. No
Christen R, Gatti J-L, Billard R. 1987. Trout sperm motility. The transient movement of trout sperm is related to changes in the concentration of ATP following the activation of the flagellar movement. Eur J Biochem, 166:667-671. No
Ciereszko A, Dabrowski K. 1994. Relationship between biochemical constituents of fish semen and fertility: the effect of short-term storage. Fish Physiol Biochem, 12:357-367. No
Comhaire F, Vermeulen L, Ghedira K, Mas J, Irvine S, Kallipolitis G. 1983. Adenosine triphosphate in human semen: a quantitative estimate of fertilizing potential. Fertil Steril, 40:500-504. No
Comhaire F, Vermeulen L, Schoonjans F. 1987. Reassessment of the accuracy of traditional sperm characteristics and adenosine triphosphate (ATP) in estimating the fertilizing potential of human semen in vivo. Intl J Androl, 10:653-662. No
Du Toit D, Bornman MS, Van Der Merwe MP, Du Plessis DJ, Oosthuizen JM. 1993. Differential sperm motility scoring and sperm ATP concentrations. Arch Androl, 30:69-71. No
Fourie MH, Du Toit D, Bornman MS, Van Der Merwe MP, Du Plessis DJ. 1991. Alpha-glucosidase, sperm ATP concentrations, and epididymal function. Arch Androl, 26:139-141. No
Gottlieb C, Svanborg K, Bygdeman M. 1991. Adenosine triphosphate (ATP) in human spermatozoa. Andrologia, 23:421-425. No
Jensen JOT, Alderdice DF. 1984. Effect of temperature on short-term storage of eggs and sperm of chum salmon (Oncorhynchus keta). Aquaculture, 37: 251-265. No
Lahnsteiner F, Patzner RA, Weismann T. 1993. Energy resources of spermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces, Teleostei). Reprod Nutr Dev, 33:349-360. No
McLaughlin EA, Ford WCL, Hull MGR. 1994. Adenosine triphosphate and motility characteristics of fresh and cryopreserved human spermatozoa. Intl J Androl, 17: 19-23. No
Moccia RD, Munkittrick KR. 1987. Relationship between fertilization of rainbow trout (Salmo gairdneri) eggs and the motility of spermatozoa. Theriogenology, 27: 679-688. No
Perchec G, Jeulin C, Cosson J, Andre F, Billard R. 1995. Relationship between sperm ATP content and motility of carp spermatozoa. J Cell Sci, 108: 747-753. No
Redondo-Muller C, Cosson MP, Cosson J, Billard R. 1991. In vitro maturation of the potential for movement of carp spermatozoa. Mol Reprod Dev, 29:259-270. No
Robitaille P-ML, Mumford KG, Brown GG. 31P nuclear magnetic resonance study of trout spermatozoa at rest, after motility, and during short-term storage. Biochem Cell Biol 1987; 65:474-485. No
Stoss J. 1983. Fish gamete preservation and spermatozoan physiology. In: Fish Physiology, Hoar W, Randall D, Donaldson E (eds). Vol 9B, pp. 305-350. Academic Press, NY. No
Stryer L. 1975. Biochemistry. W.H. Freeman and Co., San Francisco. No
Terner C. 1968. Studies of metabolism in embryonic development - I. The oxidative metabolism of unfertilized and embryonated eggs of the rainbow trout. Comp Biochem Physiol, 24: 933-940. No
Terner C, Korsh G. 1963a. The oxidative metabolism of pyruvate, acetate and glucose in isolated fish spermatozoa. J Cell Comp Physiol, 62: 243-249. No
Terner C, Korsh G. 1963b. The biosynthesis of fatty acids, of glycerides and phosphatides by isolated spermatozoa of the rainbow trout. J Cell Comp Physiol, 62: 251-255. No
Vigue C, Vigue L, Huszar G. 1992. Adenosine triphosphate (ATP) concentrations and ATP/adenosine diphosphate ratios in human sperm of normospermic, oligospermic, and asthenospermic specimens and in their swim-up fractions: lack of correlation between AT... No

Section 7. Abstract


Accurate prediction (and enhancement) of fish sperm and egg quality and fertilizing capability is very important in maximizing the fertility and the success of artificial reproduction, particularly with gametes from rare and endangered salmonid species. There are currently few reliable predictors of such characteristics. Our preliminary data suggest that the adenosine triphosphate (ATP) content of the sperm and egg is such a reliable predictor. (ATP is the universal currency of energy within cells.) Another candidate is the phosphocreatine (PCr) content as PCr can react with adenosine diphosphate to quickly generate additional ATP. Salmonid sperm are motile for less than a minute and appear to rely on stored ATP (and possibly PCr) for successful motility and fertility. Further, it is likely that the amount of stored ATP (and/or PCr) exerts an influence on energy-dependent events which occur rapidly in the egg at fertilization. Consequently, we hypothesize that there is a direct relationship between ATP, and possibly PCr, content and motility and fertilizing ability of salmonid sperm and eggs. We will expand the hypothesis to test relationship between ATP/PCr and sperm motility/fertility with cryopreserved as well as fresh samples. We predict that ATP content of frozen/thawed sperm will correlate positively with sperm function. If our hypothesis proves to be correct as our preliminary data suggest, our attention will be directed to establishing ways in which ATP (and possibly PCr) content can be maintained or even increased to enhance fertilizing success of precious samples of gametes. The results of these proposed 5-year studies should increase our basic understanding of gamete physiology of salmonids and thus be of fundamental significance. The results of this study should also support the development of methods which extend, and potentially increase, and thus enhance the fertilizing capabilities of precious samples. For example, it may be possible to increase fertilizing capability of sperm and/or eggs from poor adults, of gametes following shipment, or of cryopreserved sperm. Such a capability could be particularly valuable in increasing the chances for success in salmonid recovery programs. The results of these studies will be shared with hatchery personnel and published in peer-reviewed, scientific journals with global distributions.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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