Return to Proposal Finder FY 2000 Proposal 20048

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Viral Vaccines and Effects on Reproductive Status
BPA Project Proposal Number 20048
Business name of agency, institution,
or organization requesting funding
Washington State University
Business acronym (if appropriate) WSU

Proposal contact person or principal investigator

Name Sandra S. Ristow
Mailing Address Department of Animal Sciences
City, State, Zip Pullman, WA 99164-6351
Phone 5093350165
Fax 5093354246
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Develop a vaccine for IHNV and test its efficacy and effect on reproductive status of salmonids
Target Species Oncorhynchus mykiss (Steelhead and Rainbow trout)

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 7.2, 7.4D, (1994 Columbia River Basin Fish and Wildlife Program)
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References Endangered Species Act Section 7a2

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
1 Intracytoplasmic Sperm Injection: Genetic Retrieval Project Participant No
2 Endocrine Control of Ovarian Development in Salmonids Project Participant No
3 Analyzing Genetic and Behavioral Changes During Salmonid Domestication Project Participant No
4 Induction of Precocious Sexual Maturity and Enhanced Egg Production in Fish Project Participant No
5 Enhancement of Salmonid Gamete Quality by Manipulation of Intracellular ATP Project Participant No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Express the G and N proteins of IHNV on the surface of Yersinia ruckerii a. Construct the G expression plasmid
1. b. Express the N expression plasmid
2. Fix the recombinantly made Yesinias containing the G and N proteins with formalin or beta-propiolactone a. Fix the recombinantly made Yesinias containing the G and N proteins with formalin or beta-propiolactone

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 10/01/99 10/01/00 Express the G and N proteins of IHNV 1.0%
2 09/01/00 10/01/00 Fix the recombinantly made Yesinias containing the G and N proteins with formalin or beta-propiolactone 0.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel Sandra Ristow 1.5 Technicians, Student Timeslip $ 61,586
Fringe Sandra Ristow 26%; Technicians 29%; Timeslip 9% $ 17,330
Supplies Molecular biology supplies, restriction enzymes; Flow cytometer, Supplies $ 18,055
Operating Repair of equipment $ 3,000
Travel $ 2,000
Indirect @45% of Direct Costs (exclude equipment) $ 54,887
Other Administrative Costs $5,000; Aquaculture Core $15,000; PCR $8,685; Documentation System $19,344 $ 48,029
Total Itemized Budget $204,887

Total estimated budget

Total FY 2000 project cost $204,887
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $204,887
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

2001 2002 2003 2004
All Phases $215,131 $225,888 $237,182 $249,041
Total Outyear Budgets $215,131 $225,888 $237,182 $249,041

Other Budget Explanation

Schedule Constraints: None

Section 6. References

Reference Watershed?
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Section 7. Abstract


Infectious hematopoietic necrosis is the most important viral pathogen in the culture of steelhead trout and commercial rainbow trout. There have been a number of vaccines formulated to immunize salmonids against IHNV, but many of the products were too expensive to produce on a large scale, were limited in their effectiveness, or concerns about reversion to virulence prevented their licensure and commercialization. The purpose of this proposal is to develop a method of delivery of IHNV immunogens using the bacterium, Yersinia ruckeri, as an adjuvant carrier which will simultaneously stimulate immunity to both Y. ruckeri and to IHNV. A current vaccine strain of Y. ruckeri will be used to express the IHNV G and N genes from the pTX101 plasmid. Utilizing a rainbow trout specific pathogen free model system, we will immunize broodstock with the formalin fixed Yersinia displaying the G and N proteins of IHNV and monitor passive transfer of maternal antibody into the egg and subsequently challenge the progeny from matings (control and vaccinated) to monitor protection against IHNV. Likewise, steelhead broodstock at Dworshak National Fish Hatchery will be monitored for antibodies against IHNV and subsequently immunized with the prepared vaccine. It is anticipated that eggs from matings of vaccinated broodstock will contain antibodies against IHNV and that the progeny will be protected against an early natural challenge of IHNV.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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