Return to Proposal Finder FY 2000 Proposal 20104

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Sources of Myxobacterial Pathogens in Propagated Salmonids
BPA Project Proposal Number 20104
Business name of agency, institution,
or organization requesting funding
Abernathy Salmon Culture Technology Center/U.S. Fish & Wildlife Service
Business acronym (if appropriate) USFWS/SCTC

Proposal contact person or principal investigator

Name Dr. Peter W. Taylor
Mailing Address 1440 Abernathy Creek Rd.
City, State, Zip Longview, WA 98632
Phone 3604256072
Fax 3606361855
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Determine the sources and progression of Bacterial Cold Water Disease in propagated salmon. Develop methods to diagnose and treat this disease prior to clinical outbreaks.
Target Species coho salmon, chinook salmon and steelhead

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: 7.2A.6, 7.2D.1, .3, .4 and .6
FWS/NMFS Biological Opinion Number(s) which this project addresses: N/A
Other Planning Document References N/A

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

n/a or no information

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. methods development a. evaluate and select testing protocol
2. sampling a. collect samples, test samples
2. b. data analysis to determine control points
3. treatment selection a. test treatments

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 01/01/00 06/01/00 complete protocols 35.0%
2 08/01/00 04/01/02 collect samples 65.0%
3 03/01/02 01/01/03 test treatments 0.0%
4 01/01/03 05/01/03 final analysis 0.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel $ 26,000
Fringe $ 7,800
Supplies $ 10,000
Operating $ 5,000
Capital $ 15,000
Travel $ 5,000
Indirect $ 19,800
Other publications $ 1,500
Total Itemized Budget $ 90,100

Total estimated budget

Total FY 2000 project cost $ 90,100
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $ 90,100
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Organization Item or service provided Amount Cash or In-Kind
USFWS 0.5FTE salary $ 32,000 unknown


Outyear Budget Totals

2001 2002
All Phases $ 69,000 $ 69,000
Total Outyear Budgets $ 69,000 $ 69,000

Other Budget Explanation

Schedule Constraints: Sample collection needs to begin with a spawning season; if funds are available by 08/1999, it would begin then. If not it would begin in 08/2000.

Section 6. References

Reference Watershed?
Bader, J.A., 1995. Identification and Classification of Three Bacteria Pathogenic to Fish in the Genus Flexibacter Using the Polymerase-Chain-Reaction and Sequence Analysis on the Small Subunit (16S) Ribosomal RNA Gene. Doctoral Dissertation, Univ. of GA No
Brown, L.A., Cox, W.T., Levine, R.P. 1997. Evidence that the Causal Agent of Bacterial coldwater disease Flavobacterium psychrophilum is transmitted within salmonid eggs. Dis. Aquatic Organisns 29: 213-218 No
Chase, D.M. and Pasco, R.J., 1997. Nested PCR for Amplification of a Sequence of the p57 Gene of Renibacterium salmoninarum (abstract only). 39th Annual Western Fish Disease Workshop, Parkville, B.C. No
Cipriano, R.C., Ford, L.A., Teska, J.D., Hale, L.E., 1992. Detection of Aeromonas salmonicida in the Mucus of Salmonid Fish. J. Aquat. Animal Health 4:114-118 No
Cipriano, R.C., Schill, W.B., Teska, J.D., Ford, L.A., 1996. Epizootological Study of Bacterial Cold-water Disease in Pacific Salmon and Further Characterization of the Etiologic Agent, Flexibacter psychrophilum. J. Aquat. Animal Health 8: 28-36 No
FDA, 1984. Bacteriological Analytical Manual, 6th edition. AOAC Publications, Arligton, VA No
Ford, L.A. 1993. Detection of Aeromonas salmonicida from Water Using a Filtration Method. Aquaculture 122: 1-7 No
PNFHPC. 1997. 28th Meeting Report. Portland, OR. No
Toyama, T., Kita-Tsukamoto, K., Wakabayashi, H. 1994. Identification of Cytophaga psychrophila by PCR Targeted 16S Ribosomal RNA. Fish Pathology 29:271-275 No
Wood, J.W. 1974. Diseases of Pacific Salmon: their prevention and treatment, 2nd Edition. State of Washington, Department of Fisheries, Hatchery Division. Olympia, WA No

Section 7. Abstract


Bacterial Cold Water Disease (BCWD) and Columnaris Disease (CD) are two of the most detrimental fish health problems at hatcheries in the Pacific Northwest. Their cost, measured in numbers of fish lost and overall production dollar value, makes them the most expensive fish health problem in the region. This project proposes to look at the sources of these diseases and examine disease progression through the production cycle in hatcheries. By better defining the diseases’ source and progress during the production cycle, critical control points can be determined to reduce or eliminate the disease. Once critical control points are established, treatment options to reduce disease impact will be tested. Goals of this project address FWP goals in areas of fish health, production cost, smolt quality improvement and hatchery-wild fish interactions. The polymerase chain reaction (PCR) is a highly sensitive, species-specific method to target pathogen DNA. This test will be used to screen water, wild fish, returning spawners and eggs for the presence of BCWD. Water-hardened eggs, sac fry, alevins and fingerlings also will be screened to determine the progress of the disease throughout the hatchery production cycle. By examining a bacterial presence profile, critical control points can be selected for treatment methods to minimize the disease impact.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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