Return to Proposal Finder FY 2000 Proposal 20106

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Heritability of Disease Resistance and Immune Function in Chinook Salmon
BPA Project Proposal Number 20106
Business name of agency, institution,
or organization requesting funding
U.S. Fish and Wildlife Service
Business acronym (if appropriate) USFWS

Proposal contact person or principal investigator

Name Donald E. Campton
Mailing Address Abernathy SCTC;1440 Abernathy Creek Rd.
City, State, Zip Longview, WA 98632
Phone 3604256072
Fax 3606361855
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Determine the heritabilities and genetic correlations of resistance to bacterial kidney disease (BKD) in spring chinook salmon, and evaluate whether broodstock culling based on ELISA can cause genetic changes in disease resistance and immune function.
Target Species Chinook salmon (spring-run/stream-type), Oncorhynchus tshawytscha

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: Sections 4.2A, 7.1B, 7.2D.1, 7.2D.3, and 7.2D.4
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

n/a or no information

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Spawn spring chinook salmon (Carson National Fish Hatchery) adults in a nested design (1:6 male:female) to yield 120 full-sib (20 half-sib sire) families. [Note: Male parents will be selected to maximize the variance of their ELISA values; Female parents a. Screen 100 adult males for R. salmoninarum ELISA levels and temporarily store their milt at 4o C. Select milt from 24 males, based on their ELISA values, to fertilize 600-800 eggs from each of six, randomly selected females (144 females, 6 females/male).
2. Test HO: The proportion of chinook salmon eggs containing detectable R. salmoninarum will not differ among groups of eggs from females with different levels of R. salmoninarum infection. a. Randomly collect 50 eyed eggs from each full-sib family and store at -70oC. Separately test 30 eggs per family for the presence of R. salmoninarum by PCR amplification of DNA encoding the p57 protein of the bacterium. Perform contingency table statistical
3. Test HO: The ability of progeny to resist bacterial infections to Vibrio anguillarum does not differ among families and is not phenotypically correlated with, or compromised by, parental levels of R. salmoninarum. a. PIT-tag 72 fish (60 + 12 backups) from each full-sib family and transfer to the Western Fisheries Research Center (BRD-USGS, Seattle) for the challenge experiments. Develop challenge cultures of V. anguillarum. Challenge (a) 30 fish from each full-sib fam
4. Test HO: Humoral immune responses to (a) the p57 protein of R. salmoninarum and (b) an unrelated foreign protein do not differ among families and are not correlated phenotypically with parental levels of R. salmoninarum. a. PIT-tag 120 fish (100 + 20 backups) from each full-sib family and transfer to the Western Fisheries Research Center. Immunize 40 fish from each family with p57 (trial 1) and 40 fish from each family with an unrelated foreign protein (trial 2). Use IgM-ELI
5. Determine heritabilities and genetic correlations of all traits evaluated under Objectives 2, 3, and 4. a. Collate all the data generated by all experiments and use quantitative genetic methods to analyze those data statistically.
6. Prepare and submit final (first annual) report to BPA and scientific manuscripts for publication. .

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 01/01/00 12/01/00 Production and rearing of 120 full-sib family groups XX  
2 08/01/00 12/01/00 Tests for the presence of R. salmoninarum among 30 eyed eggs from each female parent. XX  
3 03/01/01 05/01/01 Vibrio challenge expts. XX  
4 05/01/01 08/01/01 Immunocompetency experiments XX  
5 08/01/01 10/01/01 Quantitative genetic analyses XX  
6 10/01/01 12/01/01 Prepare and submit final report XX  

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel Lead Laboratory Technician, GS-7 Laboratory Technician, GS-5 Biological Science Technician, GS-5 $ 72,195
Fringe Federal fringe benefit rate: 30% $ 21,659
Supplies Expendable supplies Antigen immunoassays Antibody immunoassays FAT/MF-FAT reagents Bacteriological c $107,600
Operating Fish maintenance (water, electricity) PIT-tagging, USFWS crew Publications and photocopying $ 36,000
PIT tags 200/family ($2.90 ea) $ 69,600
Travel Car Rental Airfare Per diem (60 person-days, $80/day) Fish transportation (truck, oxygen) $ 7,100
Indirect 22% (includes I/C on subcontracts) $ 71,932
Subcontractor Nat’l Marine Fish. Serv. $ 12,510
Total Itemized Budget $398,596

Total estimated budget

Total FY 2000 project cost $398,596
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $398,596
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

Not applicable  

Other Budget Explanation

Schedule Constraints: Spawning of spring chinook salmon begins in August. Consequently, if the proposed project is approved for funding for FY2000, we would not be able to initiate the spawning of adults until August 2000. We also need to purchase and set-up rearing tanks for maintaining 120 progeny families until those fish are large enough to PIT tag. Purchasing and setting up these tanks will require 2-3 months of lead time, and and we would need to order those tanks before the adult parents were spawned. On the other hand, if funds for FY2000 could be made available during FY1999 (e.g. by July 1, 1999), then we could begin the proposed project during the summer and fall (July-Dec) of 1999. The budget and time schedules presented in this proposal assume that funds would not be available until after October 1, 1999.

Section 6. References

Reference Watershed?
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Section 7. Abstract


Genetic effects of hatchery management practices have become a major concern in recent years. In particular, questions have been raised regarding the effects of “broodstock culling” to prevent outbreaks of bacterial kidney disease (BKD) in Columbia River hatcheries. The causative agent of BKD, Renibacterium salmoninarum, is transmitted vertically from female parent to progeny, and a common practice - particularly for spring chinook salmon - is to screen female parents for R. salmoninarum using an enzyme-linked immunoabsorbent assay (ELISA) and to segregate or destroy progeny of female parents with high ELISA values. This culling practice has proven to be a powerful tool for reducing significant losses due to BKD in spring chinook salmon during hatchery rearing and after release. However, this practice could also reduce the future fitness of these populations if disease resistance or immune function is heritable and correlated genetically with high ELISA values. Such data are currently absent but are needed to assess the possible genetic impacts of broodstock culling on long-term fitness. Information is also needed concerning the efficacy of vertical transmission of R. salmoninarum from female fish with various levels of infection. These data are critical for optimizing broodstock maintenance procedures, particularly where artificial propagation may be necessary to help recover ESA-listed populations. The proposed study will investigate vertical transmission of R. salmoninarum in spring chinook salmon, and estimate the heritabilities and genetic correlations of disease resistance indicators. Adult chinook salmon, with levels of R. salmoninarum varying throughout the range commonly observed in hatcheries, will be mated in a nested design (1:6 male:female) to yield 120 full-sib (20 half-sib) families. We will quantify levels of R. salmoninarum infection among the resulting progeny and evaluate their specific and nonspecific immune functions. Quantitative genetic methods will be used to estimate genetic correlations between level of R. salmoninarum infection in parental fish and level of infection and immune response among progeny. The proposal addresses the following research needs of the 1994 Columbia River Basin Fish and Wildlife Program: Sections 4.2A, 7.2D.1, 7.2D.3, and 7.2D.4.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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