Return to Proposal Finder FY 2000 Proposal 20111

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Preserve Cryogenically the Gametes of Selected Mid-Columbia Salmonid Stocks
BPA Project Proposal Number 20111
Business name of agency, institution,
or organization requesting funding
Columbia River Inter-Tribal Fish Commission
Business acronym (if appropriate) CRITFC

Proposal contact person or principal investigator

Mailing Address 729 NE Oregon, Suite 200
City, State, Zip Portland, OR 97232
Phone 5037311303
Fax 5032354228
Manager of program authorizing this project
Review Cycle FY 2000
Province Mainstem/Systemwide
Subbasin Systemwide
Short Description Collect and cryogenically preserve the gametes of fall chinook and/or steelhead from the Klickitat and Upper Columbia River. Transfer these gametes to a recognized qualified salmon gene banking facility.
Target Species Chinook and Steelhead

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: Section 7.4.E.1 calls for applications of Cryopreservation technology to restore and preserve depleted populations. Section 7.4.E.2 calls for demonstrations of cryopreservation identified in the coordinated habitat and production process. Section 7 of
FWS/NMFS Biological Opinion Number(s) which this project addresses: n.a.
Other Planning Document References Wy Kan Ush Me Wa Kush Wit, the Anadromous Fish Restoration Plan of the Nez Perce, Umatilla, Warm Springs and Yakama tribes calls for the reintroduction of salmon to watersheds from which they have been extirpated. While not explicitly cited as a technique of reintroduction, cryogenically preserved gametes predating the loss of a salmon stock in the wild would be a logical choice for use in a reintroduction effort. The National Marine Fisheries Service, as condition IX to required ESA permits for handling listed Snake River Salmon, requires a written statement indicating a "Willingness to Cooperate in a Cooperative Breeding Program". Cryogenic preservation is a component of Snake River salmon restoration efforts.

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous

Section 2. Past Accomplishments

Year Accomplishment
1998 Cryopreservation of wild spring chinook from the Cle Elum River

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
CRITFC Kelt reconditioning Will assist in this effort’s cryogenic component. No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. Coordinate initial steelhead and chinook salmon gamete preservation efforts with management agencies and Tribes in the Mid-Columbia region. a. Coordinate planned chinook salmon gamete preservation activities with state and Tribal management agencies.
1. b. Request and integrate agency and Tribal input into the selection of chinook and steelhead populations abundant enough to assure the success of Objective 4.
1. c. Coordinate with ongoing cryopreservation research and sampling activities.
2. Define cryopreservation project goals for gene banking of gametes from healthy chinook and steelhead populations in the Mid-Columbia region. a. Determine chinook and steelhead populations for germplasm sampling.
2. b. Review the literature and apply finding to adjust the sample sizes of Task 3.1 to those required to preserve a representative sample of the genetic diversity within the selected populations.
2. c. Assess the shortening of the range of time (years) needed to complete cryopreservation collections so that sufficient directly non-related individual genetic material is preserved.
3. Apply cryopreservation techniques to chinook and steelhead salmon conservation units at high and or mid levels of abundance. a. Follow adult sampling protocols and cryopreservation techniques for the collection, preservation, storage and inventory of male salmon germplasm. Initial goals are to preserve the gametes of 500 individual salmon from the Klickitat and mainstem Columbia
3. b. Cryopreserve adult male chinook salmon gametes from conservation units identified as being at low levels of abundance and high risk of extirpation. Assess the quality of the collections by conducting fertility tests.
3. c. To securely store the collected materials, contract with interim, and then permanent recognized salmonid gene banks in at least two independent locations.
3. d. Preserve gamete samples on-site or at the identified independent locations.
4. Transfer of Technology. a. Subcontract for the participation of two tribal fish agencies in the collection of salmon milt in the field. Encourage other fish agencies to visit and assess the applications of these techniques in other areas. Prepare and provide annual reports summa

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 04/01/00 04/01/01 Coordination leading to agreement on the cryogenic preservation of selected salmon populations in the Mid-Columbia area. Circulation of the sampling plan to the fisheries community. 10.0%
2 04/01/00 04/01/01 Identification of the sample sizes needed to preserve a representative sample of the genetic diversity within selected populations. Defined goals for gene banking of gametes in the Mid-Columbia region 5.0%
3 04/01/00 03/01/01 An ‘insurance policy’ against the loss of genetic diversity. Completed collections of straws of frozen milt safely in recognized gene bank facilities. 80.0%
4 04/01/00 04/01/01 A transfer of technology sufficient that individual biologists, agencies and tribes will be comfortable with expanding this effort. Each witnessing of the technique by those who’ve never seen it. 5.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel Fishery Scientist, .25 FTE Fishery Scientist, .1 FTE $ 13,196
Fringe @ 31.5 % $ 4,157
Supplies $ 9,000
Operating $ 600
Travel $ 2,469
Indirect $ 11,151
Subcontractor YIN, U of I, BIA $ 49,000
Total Itemized Budget $ 89,573

Total estimated budget

Total FY 2000 project cost $ 89,573
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $ 89,573
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Organization Item or service provided Amount Cash or In-Kind
Bureau of Indian Affairs Personnel $ 4,800 unknown


Outyear Budget Totals

All Phases $ 75,000
Total Outyear Budgets $ 75,000

Other Budget Explanation

Schedule Constraints: The window of opportunity for collecting milt from adult salmon in is narrow. The design of this proposal is to take milt from males which have already naturally spawned (note that the volumes of necessary for cypreservation are small, and that spent males remain an adequate source of milt for this purpose). It is envisioned that a consensus process will determine which stocks this project will ultimately select. Of the initial list, a range of schedule constraints exists: Spring chinook spawning in the Klickitat spawn peaks in late August and early September. Winter Steelhead spawning in the Klickitat occurs from March through June. Summer steelhead, if selected, spawn from January through March. Fall chinook in the Hanford Reach spawn from late October, peak in mid to late November, and taper off into December. On a different time scale, the opportunity to collect exclusively wild Spring Chinook from the Rosa Dam on the Cle Elum River will expire in the year 2000. Ideally preparation for field work should commence in mid 1999.

Section 6. References

Reference Watershed?
Ashwood-Smith, M.J. (1980). Low temperature preservation of cells, tissue, and organs. In: Low Temperature Preservation in Medicine and Biology. M.J. Ashwood-Smith and J. Farrant, eds. Pitman Medical Limited, Turnbridge Wells, Kent, Eng. pp. 19-44. No
Cloud, J. G., and C. Osborne. 1997. Cryopreservation of salmonid sperm. Department of Biological Sciences, University of Idaho. Moscow, ID No
Cloud, J. G. and G. H. Thorgaard, editors. Genetic Conservation of Salmonid Fishes. Series A: Vol. 248, NATO Advanced Science Institute Series, Plenum Press, New York and London No
Mounib, M.S. 1978. Cryogenic preservation of fish and mammalian spermatozoa. Journal of Reproductive Fertilization, 53: 13-18. No
Northwest Power Planning Council. 1994. Columbia River Basin Fish and Wildlife Program. Portland, OR. No
Stoss, J. 1983. Fish gamete preservation and spermatozoan physiology. In: Fish physiology Vol. 9 Part B. Hoar, W.S., D.J. Randall, and E.M. Donaldson eds. Academic Press, New York. pp. 305-350. No
Whittingham, D.G. 1980. Principles of embryo preservation. In: Low Temperature Preservation in Medicine and Biology. M.J. Ashwood-Smith and J. Farrant, eds. Pitman Medical Limited, Turnbridge Wells, Kent, Eng. pp. 65-83. No

Section 7. Abstract


The world faces changing environmental and political conditions, and these conditions do not always maintain native fish stocks at levels which maintain genetic diversity. Detrimental conditions causing the decreases in salmon stocks can be improved in some cases, but time is required. The 1994 Columbia Basin Fish and Wildlife Program calls for demonstrations of cryopreservation identified in the coordinated habitat and production process, as well as ensuring biodiversity. Cryopreservation of sperm is a proven technique for preserving fish germ plasm for extended periods of time (200+ years), and therefore can serve as a partial insurance against the loss of genetic diversity. Cryopreservation is already in use in programs designed to store rare fish germ plasm, but it is unapplied in the regions proposed. Biologists working with endangered stocks urge that the collection and storage of salmon gametes be conducted when stocks are at healthy levels. The end product of these approaches differ in that collections made on endangered stocks genetically sample a subset of the few individuals left in a population. The goal of this project is to demonstrate that representative genetic samples of the biodiversity from healthy populations may be economically made and preserved. This project may be monitored and evaluated based upon an assessment of the representativeness of the collections made and fertility evaluations of the stored product. Cryopreservation programs do not address habitat problems, but they are the simplest and most economical means to store genetic information contained in the DNA of male salmon from today's stocks.

Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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