Return to Proposal Finder FY 2000 Proposal 20156

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Objectives, tasks and schedules
Section 5. Budget
Section 6. References
Section 7. Abstract

Reviews and Recommendations
Title Type File Size File Date

Section 1. General Administrative Information

Title of Project Proposal Identification Of Redband And Rainbow Trout In The N F Clearwater Basin
BPA Project Proposal Number 20156
Business name of agency, institution,
or organization requesting funding
Nez Perce Tribe
Business acronym (if appropriate) NPT

Proposal contact person or principal investigator

Name Dana Weigel
Mailing Address 3404 Highway 12
City, State, Zip Orofino, ID 83544
Phone 2084769502
Fax 2084760719
Manager of program authorizing this project
Review Cycle FY 2000
Province Mountain Snake
Subbasin Clearwater
Short Description We are proposing a 12 month genetic study to identify if native wild redband trout exist in the North Fork Clearwater basin, and determine if introgression from hatchery rainbow trout threatens native redband and cutthroat trout in the basin.
Target Species redband trout, westslope cutthroat trout

Project Location

[No information]

Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal

NPPC Program Measure Number(s) which this project addresses: This INNOVATIVE NEW PROPOSAL is designed to supplement data collected in the genetic inventory of westslope cutthroat trout (9501600)measure nos 10.3C.4 and 10.3C.5, and will provide important information for loss assessments meas.nos 10.1C.1 and 10.1C.3.
FWS/NMFS Biological Opinion Number(s) which this project addresses:
Other Planning Document References

CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): resident

Section 2. Past Accomplishments

n/a or no information

Section 3. Relationships to Other Projects

Project ID Title Description Umbrella
9501600 Genetic Inventory of Westslope Cutthroat Trout in the NF Clearwater basin this proposed project will supplement information that has been acquired in 9501600 testing introgression and species distribution No
8740700 Dworshak Rule Curves this proposed project will determine if a native fish, currently not identified, exists in the NF Clearwater basin and should be considered in the evaluation of multipurpose dam operations on resident fish in Dworshak Reservoir No
9709900 Dworshak Impacts Assessment this proposed project can ultimately be used toward information supporting a consumptive fishery in Dworshak Reservoir No

Section 4. Objectives, Tasks and Schedules

Objectives and Tasks

Objective Task
1. determine if native redband trout exist in the NF Clearwater basin, and identify introgression from hatchery rainbow trout stocked into Dworshak Reservoir a. Collect tissue samples from 40-50 redband or rainbow trout from 15 sites in the basin
1. b. genetically analyze and summarize the results
2. determine if there are trends in the strain of rainbow trout hybridizing with native westslope cutthroat trout in the NF Clearwater basin a. Collect additional tissue samples from 20-30 trout from 5 sites where westslope cutthroat trout and rainbow trout introgression has been identified in the ongoing genetic inventory study
2. b. genetically analyze and summarize the results

Objective Schedules and Costs

Objective Start Date End Date Measurable Biological Objectives Milestone FY 2000 Cost %
1 06/01/00 06/01/01 determination of the number of sites and individuals with the redband genotype. This information will be related to ensuring viable populations of native trout in the basin, and the possibility of future consumptive fisheries in the basin. Annual Report summarizing the findings and relating the results to basinwide distribution data, habitat, morphological characteristics. Recommendations for resident fish mitigation in the basin 75.0%
2 06/01/00 06/01/01 determination of any identifyable trends in rainbow or redband genotypes in cutthroat X rainbow trout hybrids. This information will be related to the goals of the conservation of viable populations of native trout in the basin 25.0%

Section 5. Estimated Budget Summary

Itemized Budget

Item Note FY 2000 Cost
Personnel 6 mo Project Leader 2 seasonals 2 mo each $ 32,000
Fringe $ 8,500
Supplies office supplies, vials, racks, ethanol, waders, boots $ 2,000
Operating 4 mo rent, phone, utilities, vehicle $ 4,500
Travel field per diem, travel to AFS conference to present results $ 2,000
Indirect 23% $ 10,925
Subcontractor genetic analyses $ 51,000
Total Itemized Budget $110,925

Total estimated budget

Total FY 2000 project cost $110,925
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2000 budget request $110,925
FY 2000 forecast from 1999 $ 0
% change from forecast 0.0%

Reason for change in estimated budget

Not applicable

Reason for change in scope

Not applicable

Cost Sharing

Not applicable

Outyear Budget Totals

Not applicable  

Other Budget Explanation

Not applicable

Section 6. References

Reference Watershed?
Benke, R.J. 1992. Native trout of western North America. American Fisheries Society Monograph 6. American Fisheries Society, Bathesda, MD. No
Currens, K.P., C.B. Schreck, and H.W. Li. 1990. Allozyme and morphological divergence of rainbow trout above and below waterfalls in the Deschutes River, Oregon. Copeia. 3:730-746. No
Dowling, T. E., C. Moritz and J. D. Palmer. 1990. Nucleic acids II: restriction site analysis. In D. M. Hillis and C. Moritz (ed.) Molecular Systematics, Sinauer Associates, Inc., Sunderland. No
Felsenstein, J. 1993. PHYLIP: phylogenetic inference package. University of Washington, Seattle. No
Fitch, W. M. and M. Margoliash. 1967. Construction of phylogenetic trees. Science 155:279-284. No
McElroy, D., P. Moran, E. Bermingham and I. Kornfield. 1991. REAP: the restriction enzyme analysis package. Center for Marine Studies, University of Maine, Orono. No
Moffitt, C.M. and T.C. Bjornn. 1984. Fish abundance upstream from Dworshak Dam following exclusion of steelhead trout. Technical Completion Report. Project WRIP/371404. Idaho Waer and Energy Resources Research Institute. Univ. of Idaho, Moscow, ID. No
Morris, D.B., K.R. Richard, and J.M. Wright 1996. Microsatellites from rainbow trout (Oncorhynchus mykiss) and their use for genetic study of salmonids. Can. J. Fish. Aquat. Sci. 53:120-126. No
Nei, M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York. No
Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor. No
Spruell, P., D.L. Knudsen, J.W. Smithwick, and F.W. Allendorf. 1997. Genetic monitoring of westslope cutthroat trout in the North Fork Clearwater River. Annual Report submitted to the Nez Perce Tribe. Orofino, ID. 15pp. No
Swofford, D.L., and R. B. Selander. 1981. BIOSIS-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J. Hered. 72:281-283. No
Thurow, R.F., D.C. Lee, and B.E. Rieman. 1997. Distribution and status of seven native salmonids in the interior Columbia River basin and portions of the Klamath River and Great Basins. North American Journal of Fisheries Management. 17:1094-1110. No
Williams, R.N., D.K. Shiozawa, J.E. Carter, and R.F. Leary. 1996. Genetic detection of putative hybridization between native and introduced rainbow trout populations of the upper Snake River. Transactions of the American Fisheries Society. 125:387-401 No
Williams, R.N., R.F. Leary, and K.P. Currens. 1997. Localized genetic effects of a long-term hatchery stocking program on resident rainbow trout in the Metolius River, Oregon. North American Journal of Fisheries Management. 17:1079-1093. No
Wilson, G.M., W.K. Thomas, and A.T. Beckenbach. 1985. Intra- and interspecific mitochondrial DNA sequence divergence in Salmo: rainbow, steelhead, and cutthroat trouts. Canadian Journal of Zoology. 63:288-2094. No

Section 7. Abstract


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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