Return to Proposal Finder FY 2001 Ongoing Proposal 199009300

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget Summary

Reviews and Recommendations
No documents associated with this request


Section 1. General Administrative Information

Title of Project Proposal Genetic Analysis of Oncorhynchus nerka (Modified to include Chinook Salmon)
BPA Project Proposal Number 199009300
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI
 

Proposal contact person or principal investigator

Name Madison S. Powell
Mailing Address 3059F National Fish Hatchery Road
City, State, Zip Hagerman, ID 83332
Phone 2088379096
Fax 2088376047
E-mail fishdna@micron.net
 
Manager of program authorizing this project Madison S. Powell
 
Review Cycle FY 2001 Ongoing
Province Systemwide
Subbasin Systemwide
 
Short Description This project provides genetic information on O. nerka and O. tshawytscha throughout the Snake and Columbia Basins to be used in the overall recovery and captive propagation of endangered Snake River sockeye and threatened Salmon River chinook salmon.
Target Species


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


Biological Outcomes of this project: This project provides genetic data for returning Snake River sockeye and outmigrants to monitor the ongoing captive broodstock program. In FY2001 the project will provide more detailed genetic analyses for sockeye using microsatellite DNA markers developed in FY1999 and FY2000. In FY2001 the program will continue to develop microsatellite markers for chinook salmon and complete an assessment of mitochondrial DNA diversity among threatened Salmon River chinook salmon populations. Currently, molecular markers developed in this program are being used to construct breeding matrices for captively reared sockeye and chinook to avoid inbreeding and maximize remaining genetic diversity.
Biological Data: Each aspect of this research has either been submitted for peer-review, submitted a partial fulfillment for an academic degree (2) or submitted as an annual report. In the first two cases, the results and conclusions as well as the experimental design has been peer-reviewed by several sources. Each experimental method used has been previously published with the exception of novel microsatellite markers being tested this (FY2000) year. Numerous academic publications exist describing the merit and validity of each genetic method used and the strengths and limitations of the results obtained from each.


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

Year Accomplishment
1995 Genetic Relationships Between Maternal lineages of Sympatric Anadromous and Resident Forms of Oncorhynchus nerka determined through PCR-RFLP Analysis of mtDNA submitted as partial fulfillment of the requirement for a Master of Science degree, U of I.
1995 Development of a standardized mitochondrial DNA Restriction Fragment Length Polymorphism assay for 4 gene regions in sockeye salmon.
1995 Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1995 Genetic Analysis of Oncorhynchus nerka. Annual Report to BPA.
1996 Presentation of Redfish Lake sockeye mitochondrial data at 2 invited symposia.
1996 Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1997 Presentation of Redfish Lake sockeye mitochondrial data at BPA review.
1997 Expansion of genetic diversity analyses among sockeye to include 37 populations (including kokanee).
1997 Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1998 Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1998 Construction and analysis of a genetic breeding matrix used for captive East Fork salmon River chinook salmon.
1999 Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
1999 Genetic Analysis of threatened Salmon River Chinook Salmon (Oncorhynchus tshawytscha) Populations. Technical Report.
1999 Determination of genetic identities of returning Redfish Lake Captive Broodstock program adults and returning Sawtooth Hatchery chinook salmon.
1999 Construction and analysis of a genetic breeding matrix used for captive West Fork, Yankee Fork and Sawtooth chinook salmon.
2000 Monthly report of genetic research at sockeye and chinook salmon Technical Oversight Committee Meetings.
2000 Phylogeography of Oncorhynchus nerka in the Pacific Northwest, resubmitted for peer-reviewed publication.
2000 Review of Redfish Lake O.nerka Populations and Efforts to Recover Endangered Redfish Lake Anadromous Sockeye, submitted as partial fulfillment of the requirement for a Master of Science degree, University of Idaho.
2000 Final report for sockeye mitochondrial DNA diversity and Columbia Basin phylogeography submitted to BPA (May)


Section 3. Relationships to Other Projects

n/a or no information


Section 4. Budget for Planning and Design Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Planning and Design Phase

n/a or no information


Section 5. Budget for Construction and Implementation Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2001 cost Subcontractor
1. Monitor and evaluate Snake River O. nerka populations. a. Examine all returning sockeye, creel surveys, and trawls using mtDNA (FY2001) .15 $ 19,585  
1. b. Examine all returning sockeye, creel surveys and trawls using 4 microsatellite markers (all years). .25 $ 32,641  
1 c. Analyze available samples from Snake and Columbia River sockeye with 4 microsatellite markers. .15 $ 19,585  
2. Continue to assess mitochondrial DNA variation among Salmon River Chinook Salmon a. Complete mitochondrial DNA RFLP analysis of Salmon River Chinook Populations. .05 $ 6,528  
2. b. Compare Salmon River Chinook Populations with current data from other Columbia Basin populations. .1 $ 13,055  
3. Continue to assess nuclear genetic variation among Salmon River Chinook salmon a. Continue microsatellite DNA analysis of Salmon River Chinook Salmon. .15 $ 19,585  
3. b. Utilize microsatellite analysis for relatedness and pedigree evaluation of captive chinook salmon. .15 $ 19,585  


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Construction and Implementation Phase

FY 2004 FY 2005 FY 2002 FY 2003
$ 65,000 $ 65,000 $ 65,000 $ 65,000


Section 6. Budget for Operations and Maintenance Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Operations and Maintenance Phase

n/a or no information


Section 7. Budget for Monitoring and Evaluation Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Monitoring and Evaluation Phase

n/a or no information


Section 8. Estimated Budget Summary

Itemized Budget

Item Note FY 2001 Cost
Personnel FTE: Principle Investigator: 0.5 FTE Research Support Scientist: 1.0 FTE $ 58,344
Fringe Principle Investigator @0.285 Res. Support Scientist@0.285 $ 16,628
Supplies chemicals, pipet tips, gloves, labelled primers $ 18,100
Travel 1 professional meeting (AFS), and travel to 12 monthly T.O.C. meetings for 1 person. $ 3,200
Indirect Off campus indirect cost rate @ 20.7% $ 22,392
Capital Stratagene thermocycler and a 24 place microfuge $ 11,900
Total Itemized Budget $130,564


Total estimated budget

Total FY 2001 project cost $130,564
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2001 budget request $130,564
FY 2001 forecast from 2000 $145,000
% change from forecast - 10.0%


Reason for change in estimated budget

Microsatellite analysis will be conducted off campus at the Hagerman Fish Culture Experiment Station instead of the laboratory at Moscow. Thus, the off campus indirect cost rate (20.7%) applies instead of an indirect rate of 47%.


Reason for change in scope

NA


Cost Sharing

Not applicable
 

Outyear Budget Totals

2002 2003 2004 2005
Construction/implementation $ 65,000 $ 65,000 $ 65,000 $ 65,000
Total Outyear Budgets $ 65,000 $ 65,000 $ 65,000 $ 65,000
 

Other Budget Explanation

Not applicable


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

CBFWA Funding Recommendation Recommendation:
Ongoing Funding: yes; New Funding: no
Date:
Jul 14, 2000
2001
$130,564
Comment:
There is no change in scope of work. The reduction in budget is due to cost cutting measures. Microsatellite analysis will be conducted off campus at the Hagerman Fish Culture Experiment Station instead of the laboratory at Moscow. Thus, the off campus indirect cost rate (20.7%) applies instead of an indirect rate of 47%.

NWPPC Funding Recommendation Recommendation:
Fund
Date:
Sep 13, 2000
2001
$130,564
Comment:

NW Power and Conservation Council's FY 2006 Project Funding Review Funding category:
expense
Date:
May 2005
FY05 NPCC Start of Year:
$126,000
FY06 NPCC Staff Preliminary:
$ 98,000
FY06 NPCC July Draft Start of Year:
$ 98,000
Sponsor (U of Idaho) Comments (Go to Original on NPCC Website):

Return to top of page