Return to Proposal Finder FY 2001 Ongoing Proposal 199305600

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget Summary

Reviews and Recommendations
No documents associated with this request


Section 1. General Administrative Information

Title of Project Proposal Assessment of Captive Broodstock Technology
BPA Project Proposal Number 199305600
Business name of agency, institution,
or organization requesting funding
National Marine Fisheries Service
Business acronym (if appropriate) NMFS
 

Proposal contact person or principal investigator

Name Dr. Barry Berejikian
Mailing Address P. O. Box 130
City, State, Zip Manchester, WA 98353
Phone 2068428365
Fax 2068428364
E-mail barry.berejikian@noaa.gov
 
Manager of program authorizing this project Dr. Robert Iwamoto
 
Review Cycle FY 2001 Ongoing
Province Systemwide
Subbasin Systemwide
 
Short Description Develop captive broodstock husbandry practices through experimental research to i) improve juvenile quality, ii) improve adult reproductive performance, iii) improve in-culture survival, and iv) identify genetic risk factors.
Target Species


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


Biological Outcomes of this project: The proposed studies will increase the probability of recovery of ESA-listed salmon stocks. In-culture survival will be improved by developing new vaccines and reducing disease risks. Research to identify critical periods of imprinting should reduce straying and increase return rates of released fish to target populations. Research on adult reproductive performance is expected to identify new growth regimes and rearing conditions to maximize production from artificial spawning programs and improve reproductive success of captively reared adults released to spawn naturally. Research on inbreeding effects in captive broodstocks will determine how sensitive small populations are to the effects of inbreeding and thereby evaluate the genetic risks of captive broodstock programs.
Biological Data: This is a scientific research program. The biological outcomes will be finally determined following rigorous scientific experiments, data analyses, and interpretations. Prior research conducted under this project has generated numerous peer-reviewed scientific publications which have led to improvements in captive broodstock technology.


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

Year Accomplishment
1995 Reviewed the status of captive broodstock technology with respect to physiology, microbiology, morphology, genetics, and husbandry and produced a comprehensive written document.
1995 Determined differences in reproductive behavior and success of captively reared and wild adult salmon
1996 Developed method to bioencapsulate therapeutic levels of three antibiotic compounds into live adult Artemia.
1996 Demonstrated that hormone implants effectively advanced and synchronized spawning without impairing gamete quality in sockeye salmon
1996 Demonstrated that increased body fat levels were correlated with the onset of early (age-2) male maturity in spring chinook salmon
1996 Developed new DNA microsatellite techniques for determining individual reproductive success of spawning salmon
1997 Demonstrated that growth/body size more important than fat level in affecting early male maturity, and that high growth rate impairs cellular mediated immunity in chinook salmon
1997 Quantified individual breeding success of captively reared adults to determine correlations between morphometric variation and breeding success
1997 Demonstrated that combinations of dietary carotenoid pigments are more effective than single carotenoid supplements in developing appropriate nuptial coloration patterns
1997 Determined the optimum dietary fatty level and feeding level for sockeye salmon reared from fry to spawning, and demonstrated that there was no effect of dietary fat level on precocious maturation, unlike reports in spring chinook salmon
1997 Demonstrated higher and more consistent tissue levels of erythromycin in first feeding salmonids fed live bioencapsulated Artemia diets than conventional medicated pellets.
1998 Developed a freeze-dried bioencapsulated Artemia diet that maintains high palatability with first-feeding salmon. Freeze-dried bioencapsulated Artemia can be produced off-site, shipped and stored until needed
1998 Determined the effects of male size on female breeding behavior
1998 Provided evidence that early survival in chinook salmon can be sensitive to modest levels of inbreeding (12-25% greater than a control) incurred in a single generation
1998 Shown that chinook salmon body size and shape are highly heritable and have similar genetic and phenotypic architectures, suggesting that selection could act quickly to affect variation in these characters
1998 Demonstrated that resistance to infection by Vibrio in chinook salmon is heritable but that time to death is not. These results indicate the importance in captive broodstock programs of considering the genetic bases of traits that affect fitness
1998 Quantified individual reproductive success of captively reared adults to determine correlations between morphometric variation and breeding success. Demonstrated that kype length is correlated with behavioral measures of male breeding success.
1998 Demonstrated that diets supplemented with krill and vitamins reduced embryonic deformities in sockeye salmon.
1998 Demonstrated that rearing temperature decreased age-at-maturity in sockeye salmon
1998 Developed a panel of assays to assess immune functions of salmonid broodstock reared in captivity
1998 Completed the first quantitative profile of immune functions of sockeye salmon reared at different temperatures, and identified a rearing temperature effect on antibody responses.
1998 Demonstrated that azithromycin decreased mortality caused by Bacterial Kidney Disease, when compared to conventional (erythromycin) treatments.
1999 Quantified the effects of one consequence of early male maturation (i.e., male size) on female breeding behavior and success . Demonstrated that females respond less favorably to smaller males by delaying spawning.
1999 Directly evaluated the breeding success of captively reared adults released to spawn in their natal streams in Idaho. This research has lead to developing more effective monitoring plans and protocols, and guided reintroduction strategies
1999 Described the detailed reproductive behavior patterns of chinook salmon. This information (previously absent from the published literature) guided monitoring and evaluation efforts for programs released ESA-listed, captively reared adult chinook salmon.
1999 Described the detailed reproductive behavior patterns of chinook salmon and developed protocols for use in monitoring breeding behavior of ESA-listed chinook salmon
1999 Directly evaluated the breeding success of captively reared adults released to spawn in their natal streams in Idaho
2000 Recorded survival and growth data from brood year 1997 and 1998 inbred progeny of captively reared chinook salmon


Section 3. Relationships to Other Projects

n/a or no information


Section 4. Budget for Planning and Design Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2001 cost Subcontractor
No objectives for Planning and Design $ 0  


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Planning and Design Phase

n/a or no information


Section 5. Budget for Construction and Implementation Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2001 cost Subcontractor
1. Reduce mortality from Bacterial Kidney Disease (BKD) in chinook salmon a. Evaluation of toxic effects of long-term prophylactic use of azithromycin and erythromycin in fall chinook salmon 2 $ 55,400  
1. b. Determine azithromycin depuration or inactivation rates in effluent from salmon during feed-based antibiotic treatment. 2 $ 60,100  
1. c. Determine efficacy of vaccines 3 $ 97,750  
2. Improve adult salmon natural reproductive performance a. Evaluate the effects of rearing environments and acclimation on reproductive behavior and success 4 $198,840  
2. b. Evaluate the effects of hydraulic embryo sampling on viability 2 $ 92,600  
3. Improve reintroduction success a. Assess relative success of different re-introduction (release) strategies 4 $106,200  
4. Improve physiological development including maturation synchrony and reproductive characteristics a. Evaluate the effects of growth rate on incidence of early male maturity and adult quality in spring chinook salmon 4 $195,650  
4. b. Evaluate the effects of growth on maturation timing, fecundity, egg size, and egg quality. 4 $195,200  
5. Reduce genetic impacts of captive breeding programs a. Determine effects of close inbreeding on captive and released chinook salmon 3 $ 68,460  
5. b. Characterize genetic basis of life history in chinook salmon 3 $ 67,500 x
6. Enhance juvenile body characteristics of salmon a. Assess the quality of fish released from ongoing captive broodstock program 3 $ 48,000 x
6. b. Evaluate the effects of dietary protein and energy intakes on post-release fitness. 3 $ 52,000 x
7. Improve olfactory imprinting in sockeye salmon a. Determine environmental effects on imprinting at different developmental stages 5 $162,500 x


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Construction and Implementation Phase

FY 2004 FY 2005 FY 2002 FY 2003
$1,529,800 $1,575,700 $1,442,000 $1,485,300


Section 6. Budget for Operations and Maintenance Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Operations and Maintenance Phase

n/a or no information


Section 7. Budget for Monitoring and Evaluation Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Monitoring and Evaluation Phase

n/a or no information


Section 8. Estimated Budget Summary

Itemized Budget

Item Note FY 2001 Cost
Personnel FTE: NMFS $227,352
Fringe NMFS $ 50,699
Supplies $210,000
Travel Air, gov't vehicle, ferry, and POV $ 28,740
Indirect SLUC, NOAA $139,068
Capital Freshwater captive broodstock rearing and experimental evaluation facilities $ 48,000
PIT tags # of tags: 4000 $ 9,200
Subcontractor University of Washington $329,240
Subcontractor University of Idaho $ 87,982
Subcontractor Frank Orth and Associates $ 48,300
Subcontractor Miscellaneous Service Contracts $ 26,000
Subcontractor Pacific States Marine Fisheries Commission $162,839
Other Rents, Communication, Utilities, Printing $ 32,780
Total Itemized Budget $1,400,200


Total estimated budget

Total FY 2001 project cost $1,400,200
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2001 budget request $1,400,200
FY 2001 forecast from 2000 $1,400,000
% change from forecast 0.0%


Reason for change in estimated budget

No change


Reason for change in scope

In February 1999, project coordinators consulted with the Stanley Basin Sockeye and Snake River Chinook Technical Oversight Committees, including the project's BPA COTR, to assist in prioritizing research objectives. As a result Objective 5, Improve Olfactory Imprinting in Sockeye Salmon, was identified as critical to the success of the sockeye salmon captive broodstock program and was added to this research project. Other lower priority objectives were phased-out to accommodate this research. The remaining Objectives may appear different from the fy2000 proposal. This is because the project outline has been re-organized to better communicate the Objectives, Tasks, and products of the project in response to the fy2000 ISRP project review .


Cost Sharing

Not applicable
 

Outyear Budget Totals

2002 2003 2004 2005
Construction/implementation $1,442,000 $1,485,300 $1,529,800 $1,575,700
Total Outyear Budgets $1,442,000 $1,485,300 $1,529,800 $1,575,700
 

Other Budget Explanation

Not applicable


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

CBFWA Funding Recommendation Recommendation:
Ongoing Funding: yes; New Funding: no
Date:
Jul 14, 2000
2001
$1,400,200
Comment:
There is no change in budget.

In February 1999, project coordinators consulted with the Stanley Basin Sockeye and Snake River Chinook Technical Oversight Committees, including the project's BPA COTR, to assist in prioritizing research objectives. As a result Objective 7, Improve Olfactory Imprinting in Sockeye Salmon, was identified as critical to the success of the sockeye salmon captive broodstock program and was added to this research project. Other lower priority objectives were phased-out to accommodate this research. The remaining objectives may appear different from the FY 2000 proposal. This is because the project outline has been reorganized to better communicate the objectives, tasks, and products of the project in response to the FY 2000 ISRP project review. The MainSys SRT supports the reorganization of tasks and objectives.


NWPPC Funding Recommendation Recommendation:
Fund
Date:
Sep 13, 2000
2001
$1,300,000
Comment:
Rationale: Budget increase inappropriate in this review.

NW Power and Conservation Council's FY 2006 Project Funding Review Funding category:
expense
Date:
May 2005
FY05 NPCC Start of Year:
$1,468,100
FY06 NPCC Staff Preliminary:
$1,468,100
FY06 NPCC July Draft Start of Year:
$1,468,100
Sponsor (NOAA Fisheries) Comments (Go to Original on NPCC Website):

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