Return to Proposal Finder FY 2001 Ongoing Proposal 199703800

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget Summary

Reviews and Recommendations
No documents associated with this request


Section 1. General Administrative Information

Title of Project Proposal Preserve Salmonid Gametes
BPA Project Proposal Number 199703800
Business name of agency, institution,
or organization requesting funding
Nez Perce Tribe Department of Fisheries Resource Management
Business acronym (if appropriate) NPT
 

Proposal contact person or principal investigator

Name Robyn Armstrong
Mailing Address PO Box 1942, 125 South Mission Street
City, State, Zip McCall, ID 83638
Phone 2086345290
Fax 2086344097
E-mail robyna@nezperce.org
 
Manager of program authorizing this project Silas Whitman
 
Review Cycle FY 2001 Ongoing
Province Mountain Snake
Subbasin Salmon
 
Short Description Preserve male salmonid gametes through cryogenic techniques in order to maintain genetic diversity in populations with low levels of abundance and at high risk of localized extinction.
Target Species


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


Biological Outcomes of this project: A germplasm repository with maximum genetic diversity of threatened salmonids cryopreserved in the form of male gamete is the known biological outcome of this research. A genetic analysis report characterizing haplotypes is generated after every collection season and continually updated with new information as new markers and genetic techniques become available. Annual and quarterly project reports are available. An understanding of the population structure, genetics, and the germplasm inventory will enable managers to wisely use the gametes to rebuild depleted salmonid runs. Our technology and cryopreservation procedures are being used in current captive broodstock programs and potentially in conventional hatchery programs to ensure an adequate genetically diverse supply of male gametes. Experiments with chinook salmon have found a fertilization potential of 40% relative to fresh sperm fertilization. We are working towards a national and regional salmonid germplasm repository.
Biological Data: A total of 1,171 cryopreserved samples from Snake River basin steelhead and spring and summer chinook salmon, from 1992 through 1999 is in the germplasm repository at present. This inventory is tracked with a spreadsheet. Three annual reports are available to measure the progress of this project. We plan to evaluate the chinook salmon sampling in 2001 after 5 years of collections and evaluate the steelhead program in 2004. A minimum of five years of cryopreserving male gametes is necessary to cover one generation of different year classes. There exists a written sampling protocol for collecting male gametes in the field and at a hatchery. Fertility trials on the 1999 frozen and thawed chinook salmon sperm found a fertility potential of 40% relative to the fresh sperm fertilization.


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): anadromous


Section 2. Past Accomplishments

Year Accomplishment
1997 Cryopreserved 189 chinook salmon and 5 steelhead gametes
1998 Cryopreserved 296 chinook salmon and 25 steelhead gametes
1998 Submitted 1997 annual report to BPA
1998 Conducted chinook fertilization trials with cryopreserved versus fresh milt
1998 Thawed and fertilized Grande Ronde basin eggs with cryopreserved milt
1998 Cryopreserved 101 Grande Ronde captive broodstock chinook salmon male gametes
1999 Cryopreserved 335 chinook salmon and 208 steelhead samples
1999 Submitted 1998 annual report to BPA
1999 Conducted chinook fertilization trials with cryopreserved versus fresh milt
1999 Completed genetic analysis on 1998 chinook and 1999 steelhead cryopreserved
1999 Four large freezer tanks operational as short and long term repositories
1999 Presented project information at the Western Division American Fisheries Society meeting


Section 3. Relationships to Other Projects

n/a or no information


Section 4. Budget for Planning and Design Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Planning and Design Phase

n/a or no information


Section 5. Budget for Construction and Implementation Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2001 cost Subcontractor
1. Coordinate salmonid gamete preservation with management agencies a. Meet with other agencies who may help with the collection and/or use the cryopreserved gamete samples 3 $ 11,000  
b. Keep current of the best cryogenic technology 3 $ 8,000 x
c. Provide annual reports to NMFS summarizing project activities relating to listed salmonids under ESA 3 $ 7,000  
2. Define project goals for salmonid gene banking (5 years of collection per species @ 100 samples per aggregate) a. Identify salmonid conservation units that are at high risk of extirpation 3 $ 8,000  
b. Determine salmonid populations for germplasm sampling $ 9,000  
c. Annually evaluate sample size required per population to preserve a representative sample of genetic diversity $ 5,000  
3. Apply cryopreservation techniques to salmonid conservation units at low levels of abundance and high risk of extirpation a. Apply for needed ESA Section 10 research permits for cryopreservation activities 3 $ 6,000  
b. Utilize sampling protocols and cryopreservation techniques for the collection, preservation, storage and inventory of male salmonid germplasm 3 $ 66,000  
c. Conduct genetic analysis on fish from which sperm was cryopreserved 3 $ 20,000 x
4. Transfer of technology a. Provide quarterly and annual reports summarizing all activities associated with cryopreservation of salmonid gametes 3 $ 22,000  
b. Educate agencies, Tribes, public and other professional biologist about the salmonid gamete preservation program 3 $ 15,000  


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Construction and Implementation Phase

FY 2003 FY 2005 FY 2004 FY 2002
$1,220,000 $260,000 $240,000 $345,000


Section 6. Budget for Operations and Maintenance Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2001 cost Subcontractor
1. .Apply cryogenic techniques to salmonid conservation units at low levels of abundance and at high risk of extirpation a. Maintain germplasm repositories in at least two independent locations. 5 $ 28,000 x
b. Operate a regional germplasm repository for threatened salmonids 5 $ 15,000 x


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Operations and Maintenance Phase

FY 2003 FY 2005 FY 2004 FY 2002
$ 85,000 $170,000 $160,000 $ 75,000


Section 7. Budget for Monitoring and Evaluation Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Monitoring and Evaluation Phase

n/a or no information


Section 8. Estimated Budget Summary

Itemized Budget

Item Note FY 2001 Cost
Personnel FTE: 2 $ 75,000
Fringe 28% $ 20,000
Supplies rent, office, field supplies, telephone $ 11,500
Travel vehicles, travel, training, cryo flights $ 17,500
Indirect 26.6% of above costs $ 33,000
Subcontractor genetics lab $ 20,000
Subcontractor Univ. of ID, WA State Univ. and Nitrogen cost $ 43,000
Total Itemized Budget $220,000


Total estimated budget

Total FY 2001 project cost $220,000
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2001 budget request $220,000
FY 2001 forecast from 2000 $180,000
% change from forecast 22.2%


Reason for change in estimated budget

Indirect costs have increased about 4% from 2000. Germplasm repository operation and maintenance has increased 50% from 2000 as steelhead sample preservation has increased. Genetic analysis costs have also increased. Cryopreserving gametes from bull trout will incur additional minor costs for the universities. Some support staff were also added.


Reason for change in scope

The scope of this project has increase to cover cutthroat trout.


Cost Sharing

Not applicable
 

Outyear Budget Totals

2002 2003 2004 2005
Construction/implementation $345,000 $1,220,000 $240,000 $260,000
Operations and maintenance $ 75,000 $ 85,000 $160,000 $170,000
Total Outyear Budgets $420,000 $1,305,000 $400,000 $430,000
 

Other Budget Explanation

Not applicable


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

CBFWA Funding Recommendation Recommendation:
Ongoing Funding: yes; New Funding: no
Date:
Jul 14, 2000
2001
$213,660
Comment:
The budget has been adjusted due to a change in the indirect cost rate.

Germplasm repository O&M has increased 50% from 2000 as steelhead sample preservation has increased. Genetic analysis costs have also increased. Cryopreserving gametes from bull trout will incur additional minor costs for the universities. Some support staff were also added. The scope of this project has increased to cover cutthroat trout.


NWPPC Funding Recommendation Recommendation:
Fund
Date:
Sep 13, 2000
2001
$163,000
Comment:
Rationale: Budget increase inappropriate in this review.

NW Power and Conservation Council's FY 2006 Project Funding Review Funding category:
expense
Date:
May 2005
FY05 NPCC Start of Year:
$308,447
FY06 NPCC Staff Preliminary:
$308,447
FY06 NPCC July Draft Start of Year:
$308,447
Sponsor (Nez Perce Tribe - Lapwai) Comments (Go to Original on NPCC Website):

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