Return to Proposal Finder FY 2001 Ongoing Proposal 199902200

Proposal Table of Contents

Additional Documents

Section 1. General Administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget Summary

Reviews and Recommendations
No documents associated with this request


Section 1. General Administrative Information

Title of Project Proposal Assessing Genetic Variation Among Columbia Basin White Sturgeon Populations
BPA Project Proposal Number 199902200
Business name of agency, institution,
or organization requesting funding
University of Idaho
Business acronym (if appropriate) UI
 

Proposal contact person or principal investigator

Name Madison Powell
Mailing Address 3059F National Fish Hatchery Road
City, State, Zip Hagerman, ID 83332
Phone 2088379096
Fax 2088376047
E-mail fishdna@micron.net
 
Manager of program authorizing this project Madison Powell
 
Review Cycle FY 2001 Ongoing
Province Systemwide
Subbasin Systemwide
 
Short Description Assessing genetic variation and stock structure among white sturgeon populations in the Columbia Basin using analyses of mitochondrial and nuclear DNA.
Target Species


Project Location

[No information]


Reasonable and Prudent Alternatives (RPAs)

Sponsor-Reported Relevant RPAs

Sponsor listed no RPAs for this project proposal

Relevant RPAs based upon NMFS & BPA Review

NMFS and BPA did not associate any reasonable and prudent alternatives with this project proposal


Biological Outcomes of this project: The project, in general, will provide a comprehensive genetic assessment of white sturgeon populations throughout the Columbia Basin. In particular, the objectives and tasks undertaken in FY2001 will address nuclear variation among sturgeon populations. The first two years of the research project have examined mitochondrial DNA variation and diversity. Overall, each phase of the 3 year project builds upon the next and creates a comprehensive data set from several types of genetic analyses. This in turn provides an unparalleled examination of population structure and genetic diversity among white sturgeon systemwide.
Biological Data: Each phase of this research and the results are being submitted as academic publications in addition to annual reports. The conclusions drawn from the results as well as the merit of the experimental design will thus be peer-reviewed by several sources. Peer-review serves as the measure for significance and scientific rigor for genetic data. Peer-review is the academic process by which "biological outcomes" are generally measured


CBFWA-Generated Information

Database Administrator notes on the history of this proposal form: None
Type of Project (assigned by CBFWA Analysts): resident


Section 2. Past Accomplishments

Year Accomplishment
1998 Began examination of white sturgeon genetic diversity as a subcontract to ODFW. A copy of this preliminary report was submitted as section F of the annual report for BPA project number 86-50.
1999 Completed tasks associated with year one of project, examination of mitochondrial d-loop length variation, and submitted annual report to BPA.
1999 Preliminary genetic information was presented at either professional meetings or White Sturgeon Work Group meetings during each quarter.
1999 Organized and held a White Sturgeon Symposium during the Western Division Meeting of the American Fisheries Society.
2000 Began second year objectives for this project, DNA sequencing of the mitochondrial d-loop region.


Section 3. Relationships to Other Projects

n/a or no information


Section 4. Budget for Planning and Design Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Planning and Design Phase

n/a or no information


Section 5. Budget for Construction and Implementation Phase

Task-based Budget

Objective Task Duration in FYs Estimated 2001 cost Subcontractor
1. Survey available (published and unpublished) sturgeon microsatellite loci for variability. a. Survey 8, published microsatellite primer sets for variability among 60 samples from 5 sturgeon populations. .25 $ 34,011  
1. b. Survey up to 15 additional microsatellite primer sets (unpublished) for their utility and variability among the same sample set above. .30 $ 40,813  
2. Employ microsatellite analysis on available sturgeon DNA samples. a. Analyze available sturgeon DNA samples from 24 Columbia Basin populations with variable microsatellite primers. .33 $ 44,894  
2. b. Analyze available sturgeon DNA samples from 3 out-of-Basin populations using the same variable microsatellite loci from above to add geographic relevance. .12 $ 16,325  


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Construction and Implementation Phase

n/a or no information


Section 6. Budget for Operations and Maintenance Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Operations and Maintenance Phase

n/a or no information


Section 7. Budget for Monitoring and Evaluation Phase

Task-based Budget

n/a or no information


Outyear Objective-Based Budget

n/a or no information


Outyear Budgets for Monitoring and Evaluation Phase

n/a or no information


Section 8. Estimated Budget Summary

Itemized Budget

Item Note FY 2001 Cost
Personnel FTE: Principle Investigator: 0.5 FTE Research Support Scientist: 1.0 FTE Administrative Asst.: 0.25 FTE $ 69,867
Fringe Principle Investigator: @0.285 Research Support Scientist @0.25 Administrative Asst. @0.345 $ 18,995
Supplies Chemicals, pipet tips, tubes, labelled primers etc. $ 21,850
Travel 1 professional meeting (AFS or ASIH) and white sturgeon workgroup meetings. $ 2,000
Indirect Off campus indirect cost rate @20.7% $ 23,331
Total Itemized Budget $136,043


Total estimated budget

Total FY 2001 project cost $136,043
Amount anticipated from previously committed BPA Funds $ 0
Total FY 2001 budget request $136,043
FY 2001 forecast from 2000 $152,000
% change from forecast -10.5%


Reason for change in estimated budget

An anticipated capital acquisition (an additional PCR thermal cycler) will not be needed for completion of the project. Also, costs of labelled microsatellite primers for automated fragment analysis have decreased since the original budget estimation.


Reason for change in scope

NA


Cost Sharing

Not applicable
 

Outyear Budget Totals

Not applicable  

Other Budget Explanation

Not applicable


Reviews and Recommendations

This information was not provided on the original proposals, but was generated during the review process.

This project has not yet been reviewed

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