Within-Year Modification Request Form for FY 2008

 
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Table of Contents
Part 1. Administration and Budgeting
Section 1: General Administrative Information
Section 2: Description
Section 3: Related Projects
Section 4: Planning and Design
Section 5: Construction/Implementation
Section 6: Operations and Maintenance
Section 7: Monitoring and Evaluation
Section 8: Total Budget
Section 9: Project Documents
Part 2. Current Status of Modification Request
Part 3. Comments on this Modification Request


Part 1 of 3. Administration and Budgeting
Section 1: General Administrative Information
Process Information:
Date Proposal Submitted & Finalized Status BOG Meeting Date
January 28, 2008 Finalized February 6, 2008

Modification Type: FY 2008 Within-Year Ongoing
BPA Project Number: 200305400
BPA Project Name: Reproductive success of steelhead in the Hood River
COTR/BPA Project Manager: Tracy Hauser
Agency, Institution or Organization Requesting Rescheduling: Oregon State University
Funding Type: expense
Project Leader: Michael Blouin
Province: Lower Columbia
Subbasin: Columbia Lower
 
Contact Person
First Name: Michael
Last Name Blouin
Address: Dept. Zoology, Oregon State Univ.
City, State Zip: Corvallis, OR, 97330
Phone: 541-737-2362
Fax: 541-737-0501
Email: blouinm@science.oregonstate.edu

Administrative Contacts
Primary Administrative Contact Secondary Administrative Contact:
Name Kim Calvery Name Tara Bevandich
Address: Dept. Business Affairs, OSU Address: Dept. Zoology, Oregon State Univ.
City, State Zip: Corvallis, OR 97330 City, State Zip: Corvallis, OR 97330
Phone: 541 737 2198 Phone: 541-737-5336
Fax: 541 737 2069 Fax: 541-737-0501
Email: kim.calvery@oregonstate.edu Email: Tara.Bevandich@science.oregonstate.edu
Note: Note:

Section 2a: Description (Species Information)
Target Species Oncorhynchus mykiss (steelhead)

Provide explanations as to why the following alternative funding solutions are not feasible and will jeopardize the project:
Reduction in existing scope of project: NA - we are not asking for a change in the FY 2008 budget.
Deferral of existing work elements to later date: NA -we are not asking for a change in the FY 2008 budget.

Section 2b: Description (Location Information)
Location(s) at which the action will be implemented
Latitude Longitude Location Description
45.597 -121.636 Hood River

Is this/Are these the same study location(s) that was/were identified in the original proposal? Yes

Condition/situation creating the need for the modification:
Is the situation the result of a catastrophic event that occurred within the existing location? No
Has a habitat/population/mechanical/structural dilemma developed in the proposed study location since the contract was signed with BPA? No

Section 2c: Description (Work Element Information)
What work elements in the existing proposal does the proposed modification address?
Work Element Work Element Title
B 157 collect/generate/validate field and lab data genotyping
C 162 analyze/interpret data data analysis

Proposed action to achieve the objective(s) PLEASE SEE ATTACHED DOCUMENTATION FOR MORE DETAIL ON METHODS AND WHY WE WANT TO DO THIS.

We are not requesting any additional money. We are simply requesting permission to use part of our current FY 2008 funding to test a novel method for studying genetic differences between hatchery and wild fish.

For the rest of the 2008 contract period we propose shifting 50% of our effort to the following:

B 157 collect/generate/validate field and lab data:

(1) Use the Illumina 1G sequencer at OSU to sequence cDNA library of juvenile steelhead from the Hood River.

(2) In collaboration with staff at the Oregon Hatchery Research Center and Oak Springs Hatcheries, create pedigreed crosses within and between hatchery and wild fish for subsequent gene expression analysis.

(3) The above requires reducing the number of fish we microsatellite genotype for the year from 3000 to 1500. However, as we explain in the attached document, doing so will not interfere with the original objectives of this project for the current year because most of the fish we were going to run this year were from the backlog of old hatchery stock from the early 1990's.

C 162 analyze/interpret data:

(1) Assemble the above sequence data into a reference transcriptome for steelhead. That reference will be used to do preliminary gene expression analysis on test offspring. Based on this prelminary data, we will evaluate the potential for success of this approach for identifying the selection pressures that are causing the rapid fitness decline of hatchery fish.

(2) We will still do the reproductive success analyses on the most recent run-year of fish, as proposed originally.

Schedule for completion: End of contract period, Sept. 30, 2008.


Does not implementing this modification jeopardize the ability to achieve existing biological objectives of this project, as well as other projects? Yes

Explain: The answer is YES if the ultimate goal of our research is to find ways to improve the health and abundance of salmon stocks in the Columbia basin. Our original mission was to test the hypothesis that first-generation hatchery fish and wild fish differ in life-time fitness (reproductive success). We have now shown convincingly that fish raised in hatcheries lose reproductive fitness extremely quickly (Araki et al., 2007, Science 318: 100-103). Our current objective for the rest of fiscal year 08, and for the following year, is to continue testing that hypothesis by adding more run-years of data. We certainly intend to continue that work. However, just continuing to demonstrate, with more and more data, that hatchery fish are less fit will not lead us to a solution to the problem. Therefore, we propose changing our focus from just asking IF it occurs to begin asking WHY it occurs. Understanding the underlying genetic mechanisms that cause hatchery fish to domesticate so rapidly may eventually lead us to identify ways to modify hatchery practices to reduce the effect. Thus, we believe that we can best contribute to the long-term objectives of improving salmon abundance in the Columbia basin by exploring this new line of research.

Section 2d: Description (BiOp Information)
NMFS and/or FWS Biological Opinion that this funding request addresses Strategy
NMFS &/or USFWS
How proposed actions will address the BiOp
(i.e., substrategy / limiting factor / metric to be achieved)
NMFS 2000 hatchery Our research was originally designed to implement Action 182 under the NOAA 2000 BiOp (the need to determine the relative reproductive success of hatchery-origin and natural-origin anadromous salmonids in the Columbia basin). We have now shown that winter-run hatchery-origin steelhead in the Hood River have substantially lower reproductive success than their wild counterparts. We are continuing that work by adding more years of data, and by expanding it to include hatchery and wild summer-run fish. However, the next important question is: what are the mechanisms by which this fitness decline occurs? Here we request permission to use some of our current funding to begin exploring that important question via a study of gene expression differences between hatchery and wild fish from the Hood River.

Section 2e: Description (Objectives)
Objectives of this proposed project
Objective
(abbreviation)
Full Description
Reference transcriptome Develop a preliminary reference transcriptome for Oncorhynchus mykiss. To be accomplished by sequencing cDNA libraries from juvenile winter steelhead from the Hood River, and assembling them using heterospecific reference sequences (see attached document for further explanation).
Create test families Create families of different genetic background: HxH, HxW, WxH and WxW (where H = hatchery and W = wild). Offspring to be raised under controlled conditions and then frozen for gene expression analysis.

Section 3: Is This Funding Request Related to Other Projects in the Basin?

Other projects that are related to this request
Project # Project Title/Description Nature of Relationship
198805303 Hood River Production M&E - WS Monitoring and evaluation of Hood River production program
198805304 Hood River Production M&E - ODFW Monitoring and evaluation of Hood River production program
198805307 Hood River production O&M - WS/ODFW Operation and maintenance of Parkdale fish hatchery (acclimate and spawn adults, transfer eggs to Oak Springs hatchery)
198805308 Hood River production: Powerdale/Oak Springs Operation and maintenance of Powerdale fish trap. Adult handling and bio-sampling. Operation and maintenance of the Oak springs hatchery where the Hood River stocks are raised.

Section 4: Estimated Budget for Planning & Design phase for this request (request amount only)

Work element-based estimated budget
Planning & Design
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Planning & Design
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Planning & Design

Section 5: Estimated Budget for Contruction/Implementation phase for this request (request amount only)

Work element-based estimated budget
Contruction/Implementation
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Contruction/Implementation
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Contruction/Implementation

Section 6: Estimated Budget for Operations & Maintenance phase for this request (request amount only)

Work element-based estimated budget
Operations & Maintenance
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Operations & Maintenance
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Operations & Maintenance

Section 7: Estimated Budget for Monitoring & Evaluation phase for this request (request amount only)

Work element-based estimated budget
Monitoring & Evaluation
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Monitoring & Evaluation
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Monitoring & Evaluation

Section 8: Total Budget Modification Request

Itemized Estimated Budget
Item Note FY 2008 Cost

Total Estimated Budget Modification
Total FY 2008 budget modification $ 0
Total FY 2008 budget for this project, including modification request $277,000

Outyear Budget Totals
Not applicable

Cost sharing associated with this modification
Organization Item or Service Provided Amount ($) Cash or in-kind?

Section 9: Narrative, Maps and Project Documents
Document Type Size Date

Part 2 of 3. Current Status of Modification Request
Meeting Date Status Record Entry Date Decision Group Adjustment Category Urgency Status Budget Impact Reconsider Date
2/6/2008  1/28/2008  NA      Submitted  $ 0   
Decision Comments Next Steps
   
2/6/2008  2/6/2008  BOG  Scope  CAT-5: Other  Under BOG Review  $ 0  5/12/2008 
Decision Comments Next Steps
The request is to see if this technique would work. He can doing it within budget, but has a scope changed.  This request will be pooled for the 2nd Quarterly Review with a decision mid May. 
2/6/2008  5/15/2008  BPA  Scope    BPA Denied  $ 0   
Decision Comments Next Steps
BPA does not support this request to change scope. This method has been proposed and not funded. Council concurrs.   

Part 3 of 3. Comments on this Modification Request
There are no comments on this proposed modification.