Within-Year Modification Request Form for FY 2008

 
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Table of Contents
Part 1. Administration and Budgeting
Section 1: General Administrative Information
Section 2: Description
Section 3: Related Projects
Section 4: Planning and Design
Section 5: Construction/Implementation
Section 6: Operations and Maintenance
Section 7: Monitoring and Evaluation
Section 8: Total Budget
Section 9: Project Documents
Part 2. Current Status of Modification Request
Part 3. Comments on this Modification Request


Part 1 of 3. Administration and Budgeting
Section 1: General Administrative Information
Process Information:
Date Proposal Submitted & Finalized Status BOG Meeting Date
October 30, 2008 Finalized November 12, 2008

Modification Type: FY 2008 Within-Year Ongoing
BPA Project Number: 199305600
BPA Project Name: Research to Advance Hatchery Reform, Including Captive Broodstocks
COTR/BPA Project Manager: Greg Baesler
Agency, Institution or Organization Requesting Rescheduling: NOAA Fisheries, NWFSC
Funding Type: expense
Project Leader: Barry Berejikian
Province: Mainstem/Systemwide
Subbasin: Systemwide
 
Contact Person
First Name: Barry
Last Name Berejikian
Address: P.O. Box 130
City, State Zip: Manchester, WA 98353
Phone: 360-871-8301
Fax: 206-842-8364
Email: barry.berejikian@noaa.gov

Administrative Contacts
Primary Administrative Contact Secondary Administrative Contact:
Name Helen Brandling-Bennet Name
Address: 2725 Montlake Blvd E Address:
City, State Zip: Seattle, WA, 98112 City, State Zip:
Phone: 206-860-3232 Phone:
Fax: Fax:
Email: Helen.Brandling-Bennett@noaa.gov Email:
Note: Note:

Section 2a: Description (Species Information)
Target Species fish
Oncorhynchus nerka (sockeye)
Oncorhynchus tshawytscha (chinook)

Provide explanations as to why the following alternative funding solutions are not feasible and will jeopardize the project:
Reduction in existing scope of project: The scope of work has already been reduced to accomodate the budget reduction from the proposed amount ($1.48M) to the FY07 amount ($1.0M). The scope of work was further reduced to accomodate further budget reduction to $0.5M in FY08. The budget cannot be further reduced and still achieve the objectives that will produce important research to advance hatchery reform within the Columbia River Basin.

The Bonneville Power Administration reduced the budget for this project citing the "in lieu" issue. Based on FY 09 federal budget projections, it is now clear that NOAA will not be able to absorb further cuts in the budget for this project while producing guidance on hatchery reform for the Columbia River Basin. The goal of this project beyond FY 09 is to develop hatchery practices to reduce fitness loss in hatchery reared Chinook salmon and steelhead that may currently diminish the productivity hatchery fish in integrated supplementation and conservation programs. HSRG models currently incorporate moderate to severe fitness loss into their models of hatchery effects. The results from the current project must be completed to accurately characterize how current hatchery practices contribute to fitness loss and to propose new practices that can be tested for their effectiveness at reducing fitness loss.

Deferral of existing work elements to later date: The sample analyses requested in this modification have already been deferred as a result of the reduction in budget from $1.0M in FY07 to $0.5M in FY08. Some of the samples have a limited shelf life, and capitalizing on the investment made by BPA to this point will require funding to complete the work. The analyses must also be completed to move hatchery reform research forward and to keep pace with the need for development and implementation of Best Management Practices as called for in the 2008 BiOp.

Section 2b: Description (Location Information)
Location(s) at which the action will be implemented
Latitude Longitude Location Description
47° 27' N 122° 18' W NOAA Fisheries Northwest Fisheries Science Center

Is this/Are these the same study location(s) that was/were identified in the original proposal? Yes

Condition/situation creating the need for the modification:
Is the situation the result of a catastrophic event that occurred within the existing location? No
Has a habitat/population/mechanical/structural dilemma developed in the proposed study location since the contract was signed with BPA? No

Section 2c: Description (Work Element Information)
What work elements in the existing proposal does the proposed modification address?
Work Element Work Element Title
WE B: 157 Collect/Generate/Validate Field and Lab Data Complete experiments to maintain adaptive life history strategies in hatchery Chinook salmon
WE C: 157. Collect/Generate/Validate Field and Lab Data Complete experiments to match wild phenotypes in Chinook salmon reared in hatcheries
WE D: 157. Collect/Generate/Validate Field and Lab Data Complete experiments on sockeye salmon to assess imprinting success of juveniles

Proposed action to achieve the objective(s) With funding reinstated to the requested amount, we will complete current studies begun in FY 07 to guide hatchery reform and captive broodstocks in the Columbia River Basin. The request would simply provide for 50% of the funding authorized through Council/ISRP processes. This information is critical for guidance of operations of artificial propagation facilities. The 2008 BiOp calls for development of Best Management Practices (BMP)for hatcheries. Council and BPA programs are supporting development of Hatchery Scientific Review Group (HSRG) BMP for hatcheries to achieve their dual roles of conservation and sustainable fisheries support. A primary uncertainty for development of these needed BMP is understanding how current hatchery practices contribute to fitness loss. The Northwest Fisheries Science Center project provides a unique approach to understanding fitness loss by unifying behavioral, ecological, physiological, and molecular disciplines on problems central to hatchery reform. These approaches will help accurately characterize how current hatchery practices contribute to fitness loss and how to propose new practices that can be tested for their effectiveness at reducing fitness loss. The analyses must be completed to move hatchery reform research forward. The specific Work Elements to be completed are:

WE B: 157. Conduct experiments to maintain adaptive life history strategies in hatchery Chinook salmon. NMFS will conduct DNA pedigree analyses on offspring produced from the 2008 experiment evaluating jack and adult male breeding success (June 2009 through November 2010). Offspring will be subsampled. DNA will be amplified and genotypes of the offspring will be compared to those of the potential parents to determine the relative success of jack and adult males under different jack male frequencies. Data from the 2007 and 2008 experiments will be analyzed and incorporated into a model that will recommend Best Management Practices for the proper inclusion of jack males in Chinook salmon hatchery broodstocks. Completing this objective will provide the data needed to prepare a final report and manuscript for publication that provides specific empirically based guidance on broodstock management in Chinook salmon hatcheries(1 December 2009 through 30 April 2010).

WE C: 157. Conduct experiments to match wild phenotypes in Chinook salmon reared in hatcheries. NMFS will conduct analyses of body composition and metabolic hormones in approximately 300 samples collected from juvenile spring Chinook salmon that were from an experiment evaluating the effects of emergence timing and body growth on life history patterns (age of maturity in males and timing/age of smoltification). Samples will be analyzed June 1 2009 - 31 December 2009. Results from this analysis will provide a more indepth analysis of the how hatchery rearing strategies that either delay or accelerate salmon development affect the condition of smolts and the numbers of jacks released. Completing this objective will provide the data needed to prepare a final report and manuscript for publication 1 January 2010 - 31 May 2010) that provides recommendations on Best Management Practices for hatcheries to produce smolts with rapid downstream migration and high survival upon ocean entry, and to reduce production of minijacks.

WE D: 157. Assess imprinting success of juvenile sockeye salmon.NMFS will conduct analyses of olfactory imprint timing in Stanley Basin sockeye salmon by measuring molecular correlates of olfactory sensitivity in 250 samples collected from presmolt and smolting Stanley Basin sockeye salmon as part of an experiment examining the critical periods for olfactory imprinting in juveniles released from the Snake River sockeye safety-net Captive Broodstock program. Samples will be analyzed June 1 2009- November 30 2009. Correlative analysis of these results and smolting physiology will be conducted and reported as a final report and manuscript for publication prepared (December 1 2009 - May 31 2010). Results from this analysis will provide confirmation of imprint timing, complete validation of the imprinting assay developed under this project and allow development of Best Management Practices for release strategies utilized for recovery of Snake River sockeye and other salmon populations.


Does not implementing this modification jeopardize the ability to achieve existing biological objectives of this project, as well as other projects? Yes

Explain: The biological objectives described in this project cannot be met without the requested funding. The proposed project recieved recommendations for funding at the $1.47M level by the ISRP and CBFWA and NPCC recommendation for annual funding at $1.0M. The project was funded at 1.0M in FY 07. Funding was reduced to $500K in FY 08 with no guidance on what work elements should be removed. We terminated some work on biological objectives related to reducing BKD mortality. We reduced the scope of work on 1) sockeye salmon homing and imprinting and 2) producing wild type chinook salmon, and delayed work on 3) maintaining adaptive life history traits in Chinook salmon. The $200K currently planned for FY 09 is insufficient to bring work on these three biological objectives to completion. BPA has invested substantial funds ($1.5M) towards this important hatchery reform research. NOAA fisheries has contributed approximately $200K annually as an in kind cost share. The sample analyses, data analyses, modelling, and reporting from these studies cannot be completed without the requested funding from BPA. The BPA is depending on the Hatchery Scientific Review Group to provide guidance on hatchery reform throughout the Basin. The results of project 1993-056-00 provides empirically based guidance on hatchery reform by solving biological uncertainties in hatchery practices that the HSRG is unable to address. Research results will result best management practices recommendations, which are a key component of the 2008 BiOP with respect to artificial propagation. The two approaches are complementary and together provide the region with appropriate broodstock management practices and optimal rearing practices.

Section 2d: Description (BiOp Information)
NMFS and/or FWS Biological Opinion that this funding request addresses Strategy
NMFS &/or USFWS
How proposed actions will address the BiOp
(i.e., substrategy / limiting factor / metric to be achieved)
NMFS 2008 BiOP hatchery The analyses and reporting to be completed under this project will support Hatchery Strategies 1 and 2. All three objectives of the research project will provide recommendations to reduce the negative impacts of hatcheries and optimize the use of safety-net and conservation hatchery programs. The objective maintaining adaptive life history characteristics in Chinook salmon will guide broodstock management designed to preserve adaptive genetic diversity, and we expect the recommendations will be incorporated into best management practices. The objective to assess imprinting success of juvenile sockeye salmon has direct management implications for Hatchery Strategy 2; Actions 41 and 42 by providing guidance on which release strategies will facilitate recovery of Snake River sockeye through successful imprinting and homing.

Section 2e: Description (Objectives)
Objectives of this proposed project
Objective
(abbreviation)
Full Description
Chinook salmon life history This objective will produce management guidance for incorporating jack Chinook salmon males into spawning matrices for Columbia Basin artificial propagation programs that aim to minimize genetic divergence from the wild state. Specific recommendations on the proper use of jack and adult males in broodstock management will be made. This hatchery reform research quantifies the natural reproductive success of jack and adult Chinook salmon males and will compliment the Hatchery Scientific Review Group's guidance on broodstock management. The guidance will be based on the first ever experimental assessment of jack male breeding success under natural conditions. Eight separate groups of Chinook salmon, including varying numbers of jack males have been studied to quantify spawning behavior and breeding success. The offspring have been collected and preserved. This within-year funding request would allow for collected samples to be genotyped, statistical analyses to be conducted and recommendations developed to provide broodstock management recommendations.
Match wild phenotypes This objective will yield recommendations on Best Management Practices (rearing regimes) for juvenile spring Chinook salmon in hatchery programs that aim to produce fish with life history patterns similar to wild fish. The rearing strategies aim to reduce numbers of minijacks and produce smolts with rapid downstream migration and high survival upon seawater entry. This hatchery reform research is the first study in spring Chinook salmon to evaluate the effects of either compressing or accelerating early salmon development, which hatcheries often manipulate, on later life history events (age of male maturation and age/timing of smoltification). The fish rearing phase of the study has been completed and samples have been collected. Data on numbers of maturing males and seasonal timing of smoltification have been collected, but because of the reduced funding we will not be able to do a thorough assessment of smolt condition. The within-year funding request will provide a more indepth assessment of the condition of smolts produced by various rearing regimes. We will analyze factors such as body fat stores and metabolic hormones that reflect metabolic status of juveniles and their ability to survive the transition to seawater.
Improve imprinting and homing This objective will provide guidance on appropriate releases strategies (eyed-embryos, fry, parr, smolt) for the successful imprinting and homing of fish released as part of the Snake River sockeye Safety-Net Program and newly developed assays for imprinting will help guide general recommendations for Best Management Practices for rearing and release of hatchery fish to minimize straying. Under this objective we have developed molecular tools to assess olfactory imprinting success and complete validation of these techniques are critical before these tools can be implemented more generally. The fish-rearing phase of the validation experiments have been completed and all samples have been collected. Behavioral and physiological assessments of imprint timing have been largely completed but final analysis of the correlated molecular tools for imprinting success has not been analyzed due to the budget reductions imposed on the project. The restored funds requested would be used to complete these analyses and ensure that these new molecular tools will be available for future assessments of rearing and release practices that will minimize straying.

Section 3: Is This Funding Request Related to Other Projects in the Basin?

Other projects that are related to this request
Project # Project Title/Description Nature of Relationship
1996-067-00 Manchester Spring Chinook Captive Broodstock Data from research conducted under 1993-056-00 is communicated to captive broodstock programs through participation in Technical Oversight Committee participation. TOCs help set research priorities.
1998-010-01 Grande Ronde Captive Brood O&M Data from research conducted under 1993-056-00 is communicated to captive broodstock programs through participation in Technical Oversight Committee participation. TOCs help set research priorities.
1991-072-00 Redfish Lake Sockeye Salmon Captive Broodstock Data from research conducted under 1993-056-00 is communicated to captive broodstock programs through participation in Technical Oversight Committee participation. TOCs help set research priorities.
1992-040-00 Redfish Lake Sockeye Broodstock Data from research conducted under 1993-056-00 is communicated to captive broodstock programs through participation in Technical Oversight Committee participation. TOCs help set research priorities.
1995-063-25 Yakima/Klickitat Fisheries Project
1989-096-00 Genetic Monitoring and Evaluation Program for Salmon and Steelhead. Jack and adult male reproductive success of hatchery and wild fish are evaluated under this project. Experimental manipulation of jack ratios provides guidance for the wide range of jack male spawner ratios observed throughout the Basin
2006-014-00 Columbia River Hatchery Scientific Review The HSRG will offer broodstock management and hatchery rearing recommendations to support the FY 2008 BiOP. Our project provides complementary recommendations to further hatchery reform and optimize hatchery effectiveness within the Columbia River Basin.
Various Hatchery Supplementation Programs The results of this research will provide guidance for Chinook salmon hatchery programs throughout the Basin

Section 4: Estimated Budget for Planning & Design phase for this request (request amount only)

Work element-based estimated budget
Planning & Design
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Planning & Design
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Planning & Design

Section 5: Estimated Budget for Contruction/Implementation phase for this request (request amount only)

Work element-based estimated budget
Contruction/Implementation
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Contruction/Implementation
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Contruction/Implementation

Section 6: Estimated Budget for Operations & Maintenance phase for this request (request amount only)

Work element-based estimated budget
Operations & Maintenance
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Operations & Maintenance
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Operations & Maintenance

Section 7: Estimated Budget for Monitoring & Evaluation phase for this request (request amount only)

Work element-based estimated budget
Monitoring & Evaluation
Objective Work Element Work Element Title Work Element Description Description of Metrics Task Duration
in FYs
Estimated Budget Subcontractor?
Chinook salmon life history Collect/Generate/Validate Field and Lab Data Maintain adaptive life history characteristics in Chinook salmon Sampling, genetic processing and data analyses are required to complete assessment of jack male breeding success. Offspring of matings including jack and adult male Chinook salmon will be genotyped and assigned to the single pair matings that produced them . Pedigree results will be analyzed with behavioral data. A model will be constructed, based on these natural levels of jack male breeding, to guide the proper use of jack male Chinook salmon in Columbia River Hatcheries. Uncertainties Research FY09 $98,599 No
Improve imprinting and homing Collect/Generate/Validate Field and Lab Data Assess imprinting success of sockeye salmon juveniles Measure molecular correlates of olfactory sensitivity in 250 samples collected from presmolt and smolting Stanley Basin sockeye salmon as part of an experiment examining the critical periods for olfactory imprinting in juveniles released from the Snake River sockeye safety-net Captive Broodstock program. Results from this analysis will provide confirmation of imprint timing, complete validation of the imprinting assay developed under this project and allow development of Best Management Practices for release strategies utilized for recovery of Snake River sockeye and other salmon populations. Uncertainties Research FY09 $98,253 No
Match wild phenotypes Collect/Generate/Validate Field and Lab Data Match wild phenotypes in Chinook salmon reared in hatcheries 300 samples of juvenile salmon carcasses will be analyzed for fat, protein, ash and water content. Plasma samples from these fish will be analyzed for metabolic hormones including insulin, growth hormone and insulin like growth factor I. This information is necessary to thoroughly assess the condition of smolts produced by different rearing regimes since the ability of the fish to migrate downstream and survive depends on energy available to support migration and seawater adaptation. Uncertainties Research FY09 $101,038 No

Outyear work element-based estimated 2009 - 2012 budget for this proposal
Monitoring & Evaluation
Work Element Work Element Outyear Description Starting FY
numbers only
Ending FY
numbers only
Estimated cost

Outyear totals
Monitoring & Evaluation
FY 2009 FY 2010 FY 2011 FY 2012
$498,000 $823,000 $823,000 $823,000

Section 8: Total Budget Modification Request

Itemized Estimated Budget
Item Note FY 2008 Cost
Personnel [blank] $177,462
Supplies [blank] $29,559
Overhead [blank] $71,092
Other Contracts $19,778

Total Estimated Budget Modification
Total FY 2008 budget modification $297,891
Total FY 2008 budget for this project, including modification request $497,891

Outyear Budget Totals
  2009 2010 2011 2012
Monitoring & Evaluation $498,000 $823,000 $823,000 $823,000
Total Outyear Budgets $498,000 $823,000 $823,000 $823,000

Cost sharing associated with this modification
Organization Item or Service Provided Amount ($) Cash or in-kind?
NOAA Fisheries Utilities, Rents, Personnel $150,000 In-Kind

Section 9: Narrative, Maps and Project Documents
Document Type Size Date

Part 2 of 3. Current Status of Modification Request
Meeting Date Status Record Entry Date Decision Group Adjustment Category Urgency Status Budget Impact Reconsider Date
11/12/2008  10/30/2008  NA      Submitted  $297,891   
Decision Comments Next Steps
   
11/12/2008  11/12/2008  BOG  Budget  CAT-3b2: Loss of Monitoring and Evaluation Data  Under BOG Review  $297,891  2/16/2009 
Decision Comments Next Steps
Change in focus from strictly captive brood to hatchery reform issues, which is reflected in their '07-'09 proposal.  This request will reviewed by Fish Four in January and at the Council Meeting in February. 
11/12/2008  2/11/2009  BPA  Budget    BPA Approved  $297,891   
Decision Comments Next Steps
BPA approved with Council concurrence at 2/10/09 Council meeting in Portland. $297,891 will be added to the FY09 budget.   

Part 3 of 3. Comments on this Modification Request
There are no comments on this proposed modification.