FY 2007 Solicitation Homepage

Project Proposal Request for FY 2007 - FY 2009 Funding

Proposal 199801001: Grande Ronde Captive Brood O&M

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Table of Contents
Part 1. Administration and Budgeting
Section 1: General Administrative
Section 2: Project Location
Section 3: Project Species
Section 4: Past Accomplishments
Section 5: Relationship to Other Projects
Section 6: Biological Objectives
Section 7: Work Elements
Section 8: Budget
Section 9: Project Future
Section 10: Documents
Part 2. Reviews
Part 1 of 2. Administration and Budgeting
Section 1: General Administrative Information
Process Information:
Date Proposal Submitted & Finalized Status Form Generator
January 10, 2006 Finalized Brian Jonasson
DB Administrator's Note [Primary contact changed at request of B. Jonasson due to absent primary contact. 7/10/2006 - DBA]

Proposal Type: Ongoing
Proposal Number: 199801001
Proposal Name: Grande Ronde Captive Brood O&M
BPA Project Manager: Gregory Baesler
Agency, Institution or Organization: Oregon Department of Fish & Wildlife (ODFW)
Short Description: Captive rearing and spawning of threatened spring Chinook salmon from Catherine Creek, upper Grande Ronde River and Lostine River. Research to evaluate the effectiveness of rearing protocols and treatment and prevention of bacterial kidney disease.
Information Transfer: Data collected are available via a relational database to all program cooperators. Annual reports will be submitted to BPA and research results will be published in agency reports, presented at scientific meetings and published in scientific journals.
 
Project Proposal Contacts
Contact Organization Address Phone/Email Roles Notes
Form Submitter
Brian Jonasson Oregon Department of Fish & Wildlife 203 Badgley Hall, One University Blvd.
La Grande OR 97850
Ph: 541.962.3763
Fax: 541.962.3067
Email: bjonasso@eou.edu
Form Submitter
All Assigned Contacts
Tim Hoffnagle Oregon Department of Fish and Wildlife 203 Badgley Hall, EOU
La Grande, OR 97850
Ph: 541-962-3884
Fax: 541-962-3067
Email: tim.hoffnagle@eou.edu
Project Lead

Section 2: Project Location
Sponsor Province: Blue Mountain ARG Province: No Change
Sponsor Subbasin: Grande Ronde ARG Subbasin: No Change
Location(s) at which the action will be implemented
Latitude Longitude Waterbody Location Description County/State Subbasin Primary?
Catherine Creek Union, Oregon Grande Ronde No
Lostine River Wallowa, Oregon Grande Ronde No
upper Grande Ronde River Union, Oregon Grande Ronde No

Section 3: Focal Species
Focal Species:
Primary Secondary Additional Species
Chinook Snake River Spring/Summer ESU

Section 4: Past Accomplishments
Past Accomplishments for Each Fiscal Year of This Project
Fiscal Year Accomplishments
2005 Reared 2003 cohort Chinook salmon at Lookingglass and the 1999 - 2002 cohorts at Bonneville and Manchester. Collected 2004 cohort and spawned 1999 - 2003 cohorts. Released Captive Broodstock 2003 F1 cohort and 1999 - 2001 F1 cohorts returned as adults.
2004 Reared 2002 cohort Chinook salmon at Lookingglass and the 1998 - 2001 cohorts at Bonneville and Manchester. Collected 2003 cohort and spawned 1998 - 2002 cohorts. Released Captive Broodstock 2002 F1 cohort and 1998 - 2000 F1 cohorts returned as adults.
2003 Reared 2001 cohort Chinook salmon at Lookingglass and 1997 - 2000 cohorts at Bonneville and Manchester. Collected 2002 cohort and spawned 1997 - 2001 cohorts. Released Captive Broodstock 2001 F1 cohort and 1998 & 2000 F1 cohorts returned - first females.
2002 Reared 2000 cohort Chinook salmon at Lookingglass and the 1996 - 1999 cohorts at Bonneville and Manchester. Collected 2001 cohort and spawned 1996 - 2000 cohorts. Released Captive Broodstock 2000 F1 cohort and first return of F1s as adults.
2001 Reared 1999 cohort Chinook salmon at Lookingglass Hatchery and the 1995 - 1998 cohorts at Bonneville and Manchester. Collected 2000 cohort from program streams. Spawned 1995 - 1999 cohorts. Released Captive Broodstock 1999 F1 cohort into program streams
2000 Reared 1998 cohort Chinook salmon at Lookingglass Hatchery and the 1994 - 1997 cohorts at Bonneville and Manchester. Collected 1999 cohort from program streams. Spawned 1994 - 1998 cohorts. First release of Captive Broodstock offspring smolts.
1999 Reared 1997 cohort Chinook salmon at Lookingglass Hatchery and the 1994 - 1996 cohorts at Bonneville Hatchery and Manchester Research Station. Collected 1998 cohort from program streams. Spawned 1994 - 1997 cohorts.
1998 Reared 1996 cohort Chinook salmon at Lookingglass Hatchery and the 1994 & 1995 cohorts at Bonneville Hatchery and Manchester Research Station. Collected 1997 cohort from program streams. Spawned 1994 - 1996 cohorts - first spawn of females.
1997 Reared 1995 cohort Chinook salmon at Lookingglass Hatchery and the 1994 cohort at Bonneville Hatchery and Manchester Research Station. Collected 1996 cohort from program streams. Spawned 1994 and 1995 cohort males and cryopreserved their sperm.
1996 Reared 1994 cohort Chinook salmon at Lookingglass Hatchery and transferred some to Bonneville Hatchery for freshwater rearing and the remainder to Manchester Research Station for saltwater rearing. Collected 1995 cohort from program streams.
1995 Collected 1994 cohort Chinook salmon parr from Catherine Creek, upper Grande Ronde River and Lostine River and transported them to Lookingglass Hatchery for pre-smolt rearing under either an acclerated or simulated natural growth regime.

Section 5: Relationships to Other Projects
Other Current Projects Related to this Project (any funding source)
Funding Source Related ID Related Project Title Relationship
Other: Lower Snake River Compensation Plan [no entry] LSRCP Northeast Oregon Spring Chinook Salmon Offspring from the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program are incorporated into the LSRCP mitigation program.
BPA 198402500 Ne Oregon Habitat Projects Habitat improvement projects implemented by this project will improve habitat for offspring of Captive Broodstock Chinook salmon and increae the likelihood of success of this program.
BPA 198805301 Ne Or Hatchery Master Plan - N The Captive Broodstock Program will be directly integrated into the NE Oregon Hatcheries Program, by providing the broodstock and eggs that will be utilized by NEOH.
BPA 198805302 Ne Or Hatchery Master Plan - C The Captive Broodstock Program will be directly integrated into the NE Oregon Hatcheries Program, by providing the broodstock and eggs that will be utilized by NEOH.
BPA 198909600 Genetic M&E Prog For Sal/Steel Captive Broodstock Chinook salmon and their offspring are sampled for these important genetics studies.
BPA 199107200 Redfish Lake Sockeye Salmon Ca The Idaho sockeye salmon captive broodstock program is another similar program that uses some different methodologies and, with which, the Grande Ronde Basin Captive Broodstock Program will be compared. We meet approximately every two months to update and discuss each program.
BPA 199202601 Grand Ronde Model Watershed Watershed improvement projects implemented by the Grande Ronde Mofdel Watershed will improve habitat for offspring of Captive Broodstock Chinook salmon and increase the likelihood of success of this program.
BPA 199202604 Life Studies of Spring Chinook Success of the Captive Broodstock Program will be defined by an increase in the number of naturally spawning Chinook salmon in the program streams (F2 and subsequent generations). Information developed by Early Life History studies will assist with rearing and releasing Captive Broodstock offspring at a more natural size, condition and time to improve their survival in nature.
BPA 199305600 Demonstration of Captive Salmo This project has helped evaluate the effectiveness of captive rearing strategies, monitor fish health of Captive Broodstock salmon reared at Manchester Research Station and develop protocols to improve success of Captive Broodstock offspring released into nature.
BPA 199403300 Fish Passage Center The Captive broodstock program uses data collected by the Fish Passage Center to monitor survival and movement of the offspring through the Snake and Columbia rivers. Also, Captive broodstock progeny are part of downstream suvrvival studies conducted by the Fish Passage Center.
BPA 199606700 Manchester Spring Chinook Capt This is an integral componenent of the Captive Broodstock Program. Approximately one-half of the Grande Ronde Basin spring Chinook salmon captive broodstock are sent to Manchester Research Station for saltwater rearing to maturation.
BPA 199608300 Grand Ronde Watershed Restor Watershed improvement projects implemented by this project will improve habitat for offspring of Captive Broodstock Chinook salmon and increase the likelihood of success of this program.
BPA 199700100 Idaho Chinook Salmon Captive R The Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program is complimentary to the Idaho Chinook Salmon Captive Rearing Program. Results from the two programs will be used to develop recommendations for future captive rearing of Chinook salmon and other salmonids.
BPA 199703800 Listed Stock Chinook Salmon Ga This program allows us to cryopreserve ssperm and use it when there are insufficient numbers of fresh males to spawn with females. Cryopreserves sperm has been used extensively in the Captive Broodstock Program.
BPA 199800702 Gd Ronde Supp Lostine O&M/M&E Work and facilites funded under this project are used to rear and release Captive Broodstock offspring, as well as to enumerate them when they return as adults. Offspring from the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program are incorporated into the Lostine River supplementation program.
BPA 199800703 Grande Ronde Supp. O&M/M&E Work and facilites funded under this project are used to rear and release Captive Broodstock offspring, as well as to enumerate them when they return as adults. Offspring from the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program are incorporated into the Catherine Creek and Grande Ronde River supplementation program.
BPA 199800704 Grande Ronde Sp Chinook-Odf&W Offspring from the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program are incorporated into the Catherine Creek, upper Grande Ronde River and Lostine River supplementation programs.
BPA 199801006 Captive Broodstock Artificial The Nez perce Tribe is one of the co-management agencies for the Captive Broodstock Program (along with ODFW, CTUIR and NOAA Fisheries). This project funds M&E for the Nez Perce Tribe and is an integral part of the Captive Broodstock Program.
BPA 200106200 Lostine R Streamflow Enhanceme Enhancing streamflow in the Lostine River during adult migration will inprove habitat and survival for Lostine River Captive Broodstock offspring.
BPA 200303400 Captive Rearing For Steel/Coho This program will likely use methodolgies that have been developed and are being developed by the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program.

Section 6: Biological Objectives
Biological Objectives of this Proposed Project
Biological Objective Full Description Associated Subbasin Plan Strategy Page Nos
Enhance Natural Production of Salmonid Populations Increase numbers of naturally spawning Chinook salmon in the Grande Ronde Basin to historic levels (5,000-12,400) within 24 years (4-5 generations). Grande Ronde Use artificial production to increase the numbers of returning adults that spawn naturally. The Captive Broodstock Program is designed to rapidly increase the number of adult salmon in threatened populations, thereby reducing the threat of extinction. 272-280
Maintain Genetic Diversity in Supplemented and Uns Maintain life history characteristics and genetic diversity in supplemented and unsupplemented focal popualtions in the Grande Ronde Basin. None Operate artificial propagation programs so that genetic diversity is maintained (matrix spawning, large effective popualtion size and collect the broodstock from throughout the range, in both time and space) and life history characteristics are preserved. 272-285

Section 7: Work Elements
Work Elements and Associated Biological Objectives
Work Element Name Work Element Title Description Start Date End Date Estimated Budget
Produce Environmental Compliance Documentation Obtain NEPA Clearance and Permits Have necessary permits to conduct program. The Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program is currently operating under our Section 10 permit but are switching to a Hatchery Genetic Management Plan, which has been submitted to NOAA. 10/1/2006 9/30/2009 $10,534
Biological Objectives Metrics
No Metrics for this Work Element

Maintain Fish Health Maintain Health of Captive Broodstock Chinook Salmon Maintaining the best health possible is critical to the program, so we do all that we can to keep the Captive Broodstock salmon healthy. Prophylactic measures are taken, whenever possible, and we treat disease outbreaks aggressively to reduce the amount of horizontal transmission of the disease within each tank. Strict quarrantine measures are implemented at all times to prevent contamination between tanks. Additionally, eggs collected from females are culled for disease (particularly bacterial kidney disease) prevention based on ELISA OD values. We consult with Dr. Christine Moffitt, Univeristy of Idaho, and other fish health experts on use of drugs for treatment of disease. lastly, we are aggressively investigating, developing and evaluating innovative new methods for the detection, treatment and prevention of bacterial kidney disease, the largest source of mortality in the Captive Broodstock Program. 10/1/2006 9/30/2009 $170,348
Biological Objectives Metrics
No Metrics for this Work Element

Maintain Hatchery Maintain Fish Rearing Facilities Maintain captive broodstock facilities at Wallowa Fish Hatchery and Bonneville Fish Hatchery in good working condition - repair when necessary. 10/1/2006 9/30/2009 $11,127
Biological Objectives Metrics
No Metrics for this Work Element

Rear Fish Bonneville Fish Hatchery One half (250 minus pre-smolt mortalities) of the Captive Broodstock salmon are reared at Bonneville Fish Hatchery in freshwater from age 2 smolt to maturation (at ages 2-6). The other half of the post-smolt salmon are reared in saltwater at Manchester Research Station (NOAA Fisheries) in order to spread the risk of catastrophic mortality and to evaluate the effectiveness of saltwater vs. freshwater (Bonneville) post-smolt rearing. The salmon are reared at Manchester Research Station by NOAA Fisheries under Project Number 1996-067-00, Manchester Spring Chinook Captive Broodstock. 10/1/2006 9/30/2009 $790,451
Biological Objectives Metrics
# of smolts into program: Number collected minus pre-smolt mortalities.
* Purpose of production program: Supplementation / Gene Conservation

Rear Fish Wallowa Fish Hatchery 900 spring Chinook salmon (2004 cohort; 300 captured from each of Catherine Creek, upper Grande Ronde River and Lostine River) will be acclimated to captive conditions (environment and food) at Wallowa Fish Hatchery and reared to smoltification. We will also incubate and rear 180 eyed eggs from the 2005 cohort of the Grande Ronde River Conventional Hatchery Program for incorporation into the Captive Broodstock Program. These eggs will be from females with enzyme-linked immunosorbent assay (ELISA) optical density (OD) levels less than 0.2. All of these fish will be reared to adulthood and spawned - no fish will be euthanized. With the collection of the 2004 cohort (August 2005), we will change the experimental emphasis of the Captive Broodstock Program to evaluating methods of detecting, preventing and treating bacterial kidney disease. We will test two methods of BKD prevention at Wallowa Fish Hatchery: 1) use of parr collected from nature vs. eggs taken from a Conventional Hatchery Program (this will be conducted with Grande Ronde River salmon, beginning with the 2005 cohort) and 2) the effectiveness of a BKD vaccine vs. the standard prophylactic measures currently employeed in this program (to be conducted using Catherine Creek and Lostine River stocks). No fish will be euthanized for these evaluations. 10/1/2006 9/30/2009 $175,519
Biological Objectives Metrics
* Purpose of production program: Supplementation / Gene Conservation
# juveniles (presmolt) into program: 300 per cohort per stock

Spawn Fish Spawn Captive Broodstock Chinook Salmon at Bonneville Fish Hatchery Spawn Captive Broodstock salmon to produce the F1 generation for release into the stream from which their parents were collected. In 2006, we will be spawning maturing members of the 2001-2004 cohorts of each stock. All salmon collected from each population and cohort that reach maturity are spawned. All spawning is done using a matrix in which eggs from each female are fertilized by 2-4 males in order to maintain as much genetic diversity as possible. Males usually fertilize 2-4 females but there are often extra males, semen from which we cryopreserve for use when there are not sufficient live males. We will also collect data (see WE 157 - Collect/Generate/Validate Field and Lab Data, Collect Data for Monitoring and Evaluation Studies). 10/1/2006 9/30/2009 $55,795
Biological Objectives Metrics
* Purpose of production program: Supplementation / Gene Conservation
* # of Female non-clip (natural origin) fish: ~ 250 minus rearing mortalties
* # of Male non-clip (natural origin) fish : ~250 minus rearing mortalities

Trap/Collect/Hold/Transport Fish - Hatchery Collect 300 Naturally Produced Chinook Salmon Parr / cohort / stock Collect 900 2004 cohort spring Chinook salmon (Snake River ESU) parr (300 from each of Catherine Creek, upper Grande Ronde River and Lostine River). These are the standard Captive Broodstock collections for production, although less than previous years. We will also collect 180 Chinook salmon eyed eggs from the 2005 cohort of the Grande Ronde River Conventional Hatchery Program for incorporation into the Captive Broodstock Program. These eggs will be from females with enzyme-linked immunosorbent assay (ELISA) optical density (OD) levels less than 0.2 and will be used to compare the effectiveness of collecting eggs vs. wild parr for use in captive broodstock programs. All of these fish will be reared to adulthood and spawned - no fish will be euthanized. Also, transport captive broodstock Chinook salmon smolts from Wallowa Fish Hatchery to either Bonneville Fish Hatchery or Manchester Research Station and maturing adults from Manchester to Bonneville. 10/1/2006 9/30/2009 $134,968
Biological Objectives Metrics
* # of non-clip (natural origin) juveniles (presmolt): 300 per cohort per stock
* Purpose of production program: Supplementation / Gene Conservation
* # of non-clip (natural origin) juveniles (presmolt): Collect 180 eyed eggs from conventional broodstock

Coordination Program Coordination Coordinate program activities with co-managers within the Captive Broodstock Program and with BPA and simiilar regional programs. Restricted funding will affect the level of coordination for the program. 10/1/2006 9/30/2009 $36,124
Biological Objectives Metrics
No Metrics for this Work Element

Manage and Administer Projects Manage project and Prepare FY06 SOW with budget Provide oversight of the project and respond to BPA as requested providing financial, contractual, and administrative documents. Prepare FY 06 Statement of Work for submission to BPA no less than 90 days before end of current performance period. 10/1/2006 9/30/2009 $78,266
Biological Objectives Metrics
No Metrics for this Work Element

Produce Plan Produce Plans for Conducting the Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program Develop annual operating plans for guidance of the Captive Broodstock Program through each year. 10/1/2006 9/30/2009 $57,195
Biological Objectives Metrics
No Metrics for this Work Element

Provide Technical Review Provide Technical Review of Captive Broodstock/Propagation Projects Provide technical review of captive broodstock/propagation projects, as requested. 10/1/2006 9/30/2009 $5,269
Biological Objectives Metrics
No Metrics for this Work Element

Produce Annual Report Development and Submission of Annual Report Prepare and submit an annual report documenting all acitivities conducted during the year 10/1/2006 9/30/2009 $118,576
Biological Objectives Metrics
No Metrics for this Work Element

Produce Pisces Status Report Quarterly Reporting Provide oversight of the project and respond to BPA as requested providing financial, contractual, and administrative documents. Prepare FY 06 Statement of Work for submission to BPA no less than 90 days before end of current performance period. 10/1/2006 9/30/2009 $10,534
Biological Objectives Metrics
No Metrics for this Work Element

Produce/Submit Scientific Findings Report Disseminate Findings Through Scientific Presentations and Publications Disseminate the results and findings of the Captive Broodstock Program through presentations at scientific meetings and publications of agency reports and in scientific journals as sufficient data become available. 10/1/2006 9/30/2009 $21,071
Biological Objectives Metrics
No Metrics for this Work Element

Analyze/Interpret Data Analyze Data to Evaluate Rearing Protocols, Spawning, Genetic Relatedness and Experimental Treatments Analyze and interpret data collected on growth, health, survival, maturation and spawning of captive broodstock salmon reared under either an accelerated or simulated natural presmolt growth regime and freshwater vs. saltwater post-smolt rearing and their effects on spawning parameters. Draw conclusions from analysis results that will suggest which rearing method is best suited for captive broodstock programs. Also analyze data to examine genetic relatedness of the captive broodstock and the effectiveness of erythromycin vs. azithromycin to prevent vertical transmission of BKD. 10/1/2006 9/30/2009 $84,280
Biological Objectives Metrics
Primary R, M, and E Type: Action Effectiveness Research

Collect/Generate/Validate Field and Lab Data Collect Data for Monitoring and Evaluation Analyses Collect data to evaluate growth, health, survival to maturation, age and size at maturation, fecundity and other spawning characteristics for individuals of each treatment group - accelerated vs. natural presmolt growth regimes and freshwater vs. saltwater post-smolt rearing. Conduct lab analyses to determine cause of death of mortalities and health status of spawned salmon. Bacterail kidney disease (BKD) is the largest source of mortality in the Captive Broodstock Program. Since a goal of the program is to develop protocols for use in future captive broodstock programs and an M&E objective is to develop and evaluate new methodologies, we are now shifting the focus of the program to finding new ways to combat this disease and its effects. We have developed four evaluations. 1) Vertical Transmission of BKD - We collected kidney and ovarian fluids samples from all females spawned in 2005 for analysis by three methods and will rear some of their offspring and observe for develpment of BKD. We can then determine which detection method does a better job of predicting the likelihood of vertical transmission of BKD. This may help us reduce the amount of BKD in the F1 generation and reduce the amount of unnecessary culling of eggs for BKD prevention. 2) Erythromycin vs. Azithromycin - We will test the effectiveness of each of these drugs to reduce vertical transmission of BKD. We collected 200 eggs and a kidney sample from all females spawned in 2005 for analysis of drug content of the tissues and analysis for BKD. 3) BKD Vaccine - We are also testing an experimental BKD vaccine on the 2004 cohort parr from Catherine Creek and the Lostine River and will continue the evaluation using some offspring from high ELISA females from the 2005 cohort. We will compare the effectiveness of the BKD vaccine against the standard prophylactic treamtment that we have been using in the Captive Broodstock Program. 4) Parr vs. Eggs - We will compare the use of natural parr vs. collection of eggs from the conventional hatchery program for use in a captive broodstock program. We will compare growth, health, survival and genetic relatedness among the individuals in each cohort. 10/1/2006 9/30/2009 $788,962
Biological Objectives Metrics
Primary R, M, and E Type: Action Effectiveness Research

Create/Manage/Maintain Database Manage and Maintain Captive Broodstock Database The captive broodstock database is maintained and managed by ODFW. It is a relational database that contains all data collected during captive broodstock activities and is accessible to all individuals from the co-managment agencies (ODFW, NPT, CTUIR and NOAA Fisheries) that are involved in the Captive Broodstock Program. In the near future, this database will be linked to another database that will be used to evaluate the performance of captive broodstock offspring in captivity and in nature for comparisons with natural and conventional hatchery offspring. 10/1/2006 9/30/2009 $9,299
Biological Objectives Metrics
No Metrics for this Work Element

Develop RM&E Methods and Designs Develop RM&E Methods and Designs for the Captive Broodstock Program Develop Research, Monitoring and Evaluation methods and designs to evaluate the Captive Broodstock Program and its research elements. Also develop research plans and experimental designs to evaluate issues of importance to the Captive Broodstock Program - these issues may also have applicability beyond captive broodstock/rearing programs, e.g., the detection, treatment and prevention of BKD and the use of ultrasound and near infrared spectroscopy for early detection of maturation and sex.. 10/1/2006 9/30/2009 $21,071
Biological Objectives Metrics
No Metrics for this Work Element

Mark/Tag Animals Tag Captive Broodstock Chinook Salmon Each Captive Broodstock salmon (up to 1,500 per cohort) will be individually tagged with both a PIT tag and VI tag so that we can monitor individual growth, health and maturation. The VI tags will provide backup identification to the PIT tags to insure that individuals can be identified. Jaw tags are applied to maturing salmon due to the possibility of loss of PIT and/or VI tags during maturation. 10/1/2006 9/30/2009 $25,101
Biological Objectives Metrics
Primary R, M, and E Type: Action Effectiveness Research


Section 8: Budget

Itemized Estimated Budget
Item Note FY 2007 Cost FY 2008 Cost FY 2009 Cost
Personnel M&E, hatchery, fish health and fish transport staff $330,642 $347,174 $364,533
Fringe Benefits M&E, hatchery, fish health and fish transport staff $163,534 $171,711 $180,297
Supplies Field, lab and office supplies $52,918 $54,506 $56,141
Travel includes vehicle costs $41,952 $43,210 $44,507
Other rent and utilities $10,970 $11,299 $11,638
Capital Equipment There may be foreseeable but unpredictable capital costs that have not been accounted for in any of these budgets, such as replacement of filter systems, pumping systems or tanks or roofing repairs at Bonneville Fish Hatchery or Wallowa Fish Hatchery. $14,008 $14,428 $14,861
Overhead 35.87% $215,226 $225,228 $235,707
Totals $829,250 $867,556 $907,684

Total Estimated FY 2007-2009 Budgets
Total Itemized Budget$2,604,490
Total Work Element budget$2,604,490

Cost sharing
Funding Source or Organization Item or Service Provided FY 2007 Est Value ($) FY 2008 Est Value ($) FY 2009 Est Value ($) Cash or in-kind? Status

Section 9: Project Future
Project Future Costs and/or Termination
FY 2010 Est Budget FY 2011 Est Budget Comments
$949,718 $993,755 annual budget increased from 2007 - 2011 by 5% for Personnel and Fringe Benefits (as dictated by union contract) and 3% for other costs
Future Operations & Maintenance Costs
There are likely to be foreseeable but unpredictable capital costs that have not been accounted for in any of these budgets, such as replacement of filter systems, pumping systems or tanks or roofing repairs at Bonneville Fish Hatchery or Wallowa Fish Hatchery. Also, our fish transport vehicles are ageing and may need to be replaced, which will cost approximately $60,000.
 
Termination Date Comments
 
Final Deliverables

Section 10: Narrative
Document Type Size Date

Part 2 of 2. Reviews of Proposal
Administrative Review Group (ARG) Results
Account Type:
Expense
No changes were made to this proposal


NPCC Final Funding Recommendations (October 23, 2006) [Full NPCC Council Recs]

FY 2007 Budget
$723,718
FY 2008 Budget
$723,718
FY 2009 Budget
$723,718
Total NPCC Rec
$2,171,154
Budget Type:Expense
Budget Category:ProvinceExpense
Recommendation:Fund
Comments: ISRP fundable in part (qualified). No additional parr collection, as per ISRP recommendation, and to continue and complete the captive propagation experiment Budget reduction reflects the removal of the work element task titled - Collect 300 Naturally Produced Chinook Salmon Parr / cohort / stock. Other budget reductions not specific. Project to be implemented as proposed with reduced scope, other than what is specified.


NPCC Draft Funding Recommendations (September 15, 2006) [Full NPCC Council Recs]

FY 2007 Budget
$723,718
FY 2008 Budget
$723,718
FY 2009 Budget
$723,718
Total NPCC Rec
$2,171,154
FY 2007 MSRT Rec
$ 0
FY 2008 MSRT Rec
$ 0
FY 2009 MSRT Rec
$ 0
Total MSRT Rec
$ 0
Budget Category:ProvinceExpense
Comments:
NPCC Staff Comments: ISRP fundable in part (qualified). No additional parr collection as per ISRP recommendation.

Local or MSRT Comments: OSPIT recommends holding the budget to the FY06 level and flatlining for FY08 and 09.


Independent Scientific Review Panel Final Review (August 31, 2006) [Download full document]

Recommendation: Fundable in part (Qualified)
NPCC Comments: Fundable-in-part to continue and complete the captive propagation experiment. The project has already continued to collect parr beyond the timeframe that was initially envisioned. Additional parr collections do not seem justified. This project should be limited to raising the parr they now have collected, and completing the envisioned experiment.

The ISRP recommendation is Qualified in that the design of the final analysis needs to be capable of quantifying the demographic effect of natural spawning by captive propagated and supplementation adults on natural production in the next generation.

Several concerns were raised in the preliminary ISRP review. First it was not clear from the proposal that an increase in the abundance of natural-origin adult Chinook is a goal of this project, and the ISRP response stated that the ISRP believes it should be. Second, the proposal implies that a sustained production of wild fish is a goal; however, since the 150,000 smolts with an SAR of 0.1% is a goal from fish production, the ISRP response stated that "it is not clear how these fish are being called wild Chinook salmon," and that "the critical benefit to the focal species would be an increase in the numbers of natural-origin adults in the treatment stream, not just increases in the numbers of hatchery-origin adults.”

The preliminary ISRP review then asked the sponsors to address four questions. The following is an assessment of the quality of their responses.

1. Identify the method by which they will determine the demographic benefit to the focal species – in terms of an increase in the numbers of natural-origin adults.

Their response was very thorough: "We are using a variety of approaches to assess the natural-origin demographic benefit provided by natural spawning hatchery fish, including: comparing demographic trends … comparing pre- and post-treatment smolts produced per parent in all three treatment streams."

2. Outline the 19-year experiment identified in the December 19, 2003 document and clarify when juvenile (parr) collections are no longer needed to support that experiment.

They did that, but explained "we continued to collect parr because we had not met our goal of a consistent return of 150 adults spawning in nature and had problems with the experimental component that required more brood years of parr collection for captive rearing (see below)." Given that this is an experiment, this seems unjustified. We emphasize that a demographic benefit from this program is yet to be demonstrated. On that basis, we encourage the co-managers to seriously consider the option of phasing out the captive propagation program in the Lostine system, per the discussion in the response to the ISRP.

3. If that timeframe is already passed provide justification for additional collections.

The response, "We had not achieved our threshold annual goal of a consistent return of 150 adults spawning in nature in the Catherine Creek and Grande Ronde River populations at the time we collected the 1999 brood year parr (Table 1)."

Sponsors have passed the timeframe for collecting parr for their experimental evaluation of captive propagation and continue to collect parr, albeit at a reduced number. The rational for continuing the collections is that the target populations have not yet reached the abundance thresholds decided upon by the co-managers. A decision on the efficacy of the captive propagation technology will be decided in 2014 after the data collection and analysis from the 19-year experimental phase is concluded. Further, the ISRP encourages the timely reporting of the demographic response of the target populations as the data becomes available.

4. Identify the timeframe for providing a reasonable demographic benefit to the focal species or the technology would be judged ineffective.

Their response was straightforward: "We plan to follow the schedule of the 19 year experiment to determine whether the Captive Broodstock program can increase the number of natural spawners." That is encouraging.

The authors, however, did add the following:

"At that time, we will have adequate data to assess relative reproductive success of the Captive Broodstock F1 generation, trends in total population abundance, recruits:spawner ratios, total adults spawning in nature and abundance of natural origin adults and compare these within streams in pre- vs. post-supplementation comparisons and with unsupplemented reference streams. The evaluation of the reproductive success of the Captive Broodstock offspring will also be done genetically by identifying the F2 generation on the spawning grounds. We will also measure and compare variables such as survival, size and age at maturation, sex ratio, migration and spawn timing, and spawning distribution."

The ISRP concern is that the DNA pedigree analysis should provide the way to conduct the evaluation – but it not sufficiently clear what is to really be done. Because that information is the key to determining the real level of success of the captive propagation experiment underway, that methodology could/should be explained in detail.


Independent Scientific Review Panel Preliminary Review (June 2, 2006) [Download full document]

Recommendation: Response requested
NPCC Comments: The Grande Ronde Captive Broodstock Operations and Maintenance project collects natural parr from Catherine Creek, the Grande Ronde River, and the Lostine River, rears these individuals to adulthood in either freshwater or saltwater, spawns them, and then rears their progeny to the smolt stage for release back into the three natal streams. The smolts are intended to migrate to the ocean and then return as adults and then finally reproduce. The project has achieved modest success at rearing the natural parr to adults, but has fallen short of the goal of releasing 150,000 smolts into each stream because of BKD mortality. There have also been modest returns of the hatchery produced smolts as adults to the three treatment streams.

The historical description in the proposal was helpful, particularly the presentation of past findings. The summary of the project and the returns of adult fish are welcome. The summary indicates that returning adult fish have exceeded projections for 1998 and 1999 cohorts, but no breakdown by sex is given. If these encouraging results are largely male jacks, the outcome is not as favorable as the raw number would suggest. The report that > 95% of the adults in the Grande Ronde River in 2004 and 2005 are from the Captive Broodstock program would support the need for this type of action, but this also raises concern about inbreeding in the population. The program had better returns in the first years than later, and the release of 200,000 smolts from the 2001 spawn year produced only 58 adults whereas a release of 151,000 from the 2000 spawn year produced 546 adults. This suggests that some potential to reduce the risk of demographic collapse is possible, but even with a captive brood program, the populations can fall to quite low numbers.

Most of the methods used to produce the fish are adequately described in the proposal. There are experiments imbedded in the work elements 1) assess and compare the effects of the natural and accelerated pre-smolt rearing; 2) assess and compare the effects of the freshwater and saltwater post-smolt rearing; 6) assess the effectiveness of matrix spawning protocols and success at meeting performance benchmarks. Project monitoring and evaluation is performed under the Lower Snake River Compensation Plan (LSRCP). Specific details are not presented, but the general framework appears adequate. The sponsors have a long history of collecting data on juvenile and adult salmon so there is no reason to question the reliability of the data. The project sponsors have produced useful reports in the past, and it is likely they will in the future.

On this basis this it is reasonable to consider continuation of this project; however, there are several elements that need clarification, so the ISRP recommends a response.

First, a number of statements in the technical and scientific background raise questions for the ISRP.

Sponsors state that "Program success is dependent on achievement of adequate survival, growth, maturation, gamete viability, smolt-to-adult survival and reproductive success," this is followed by eight assumptions that the project operates under, followed by "These became the benchmarks for success of the Captive Broodstock Program and are the targets against which we have been and will evaluate the program." The next sentence states "The Grande Ronde Basin Chinook Salmon Captive Broodstock Program was developed to achieve a sustained annual return of at least 150 wild Chinook salmon to each program stream (150,000 smolts and a smolt-to-adult return rate, SAR, of 0.1%)."

It is not clear from the first statement and eight assumptions that an increase in the abundance of natural-origin adult Chinook is a goal of this project, and the ISRP believes it should be. The second statement implies that a sustained production of wild fish is a goal; however, since the 150,000 smolts with an SAR of 0.1% is a goal from fish production, it is not clear how these are being called wild Chinook salmon.

The critical benefit to the focal species would be an increase in the numbers of natural-origin adults in the treatment stream, not just increases in the numbers of hatchery-origin adults. So, sponsors need to:

1. Identify the method by which they will determine the demographic benefit to the focal species – in terms of an increase in the numbers of natural-origin adults.

Second, in the December 19, 2003 Review of Blue Mountain and Mountain Snake Province Captive Propagation Programs: Response to the Northwest Power and Conservation Council, the sponsors of the Grande Ronde project identified that they planned 5 generations of captive broodstock that would take 19 years to complete a full data collection and analysis. The sponsors, however, both in explicitly that document, and implicitly in their current proposal by failing to identify a termination date, indicate they will continue to use captive broodstock technology until they return sufficient adults to the treatment streams. The ISRP recommended in the review of the December 19, 2003 document that they should adhere to the 19-year experimental design.

Consequently, sponsors need to:

2. Outline the 19 year experiment identified in the December 19, 2003 document and clarify when juvenile (parr) collections are no longer needed to support that experiment;

3. If that time frame is already passed provide justification for additional collections;

4. Identify the timeframe for providing a reasonable demographic benefit to the focal species or the technology would be judged ineffective.

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