FY 2002 Mountain Snake proposal 199107200

Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary

Reviews and Recommendations

Additional documents

Title	Type
199107200 Narrative	Narrative
199107200 Sponsor Response to the ISRP	Response
199107200 Powerpoint Presentation	Powerpoint Presentation
Mountain Snake: Salmon Subbasin Map with BPA Fish & Wildlife Projects	Subbasin Map
Mountain Snake: Salmon Subbasin Map with BPA Fish & Wildlife Projects	Subbasin Map

Section 1. Administrative

Proposal title	Redfish Lake Sockeye Salmon Captive Broodstock Program
Proposal ID	199107200
Organization	Idaho Department of Fish and Game and Idaho Office of Species Conservation (IDFG/IOSC)
Proposal contact person or principal investigator
Name	Paul A. Kline
Mailing address	1800 Trout Road Eagle, ID 83616
Phone / email	2089394114 / pkline@idfg.state.id.us
Manager authorizing this project	Steve Yundt
Review cycle	Mountain Snake
Province / Subbasin	Mountain Snake / Salmon
Short description	Establish captive broodstocks of Redfish Lake sockeye salmon. Spawn captive adults to produce eggs, juveniles, and adults for reintroduction and future broodstock needs. Evaluate juvenile out-migration and adult returns by release option.
Target species	Snake River sockeye salmon

Project location

Latitude	Longitude	Description
44.1517	-114.8843	Sawtooth Hatchery - centrally located in project area

Reasonable and Prudent Alternatives (RPAs)

Sponsor-reported:

RPA
Action 175
Action 177

Relevant RPAs based on NMFS/BPA review:

Reviewing agency	Action #	BiOp Agency	Description
NMFS	Action 176	NMFS	BPA shall, in coordination with NMFS, USFWS, and the relevant state and Tribal comanagers, fund the development of HGMPs for the Grande Ronde and Tucannon spring/summer chinook safety-net programs.
NMFS	Action 177	NMFS	In 2002, BPA shall begin to implement and sustain NMFS-approved, safety-net projects.
NMFS/BPA	Action 177	NMFS	In 2002, BPA shall begin to implement and sustain NMFS-approved, safety-net projects.

Section 2. Past accomplishments

Year	Accomplishment
1991	Captured four anadromous adults (three males, one female) at Redfish Lake Creek trap - held for spawning.
	Captured over 759 wild sockeye salmon outmigrants at Redfish Lake Creek trap - transferred to Eagle Fish Hatchery.
	Developed first broodstock from the four anadromous adult returns (BY91 spawning).
	First cryopreservation of sockeye salmon milt (from three male returns).
	Excellent rearing survival of wild-captured Redfish Lake outmigrants at the Eagle Fish Hatchery.
	Primary facility improvements made to Eagle Fish Hatchery to accommodate conservation hatchery protocols.
1992	Captured single male anadromous adult at Redfish Lake Creek trap - held for cryopreservation.
	Captured 79 wild sockeye salmon outmigrants at Redfish Lake Creek trap - transferred to Eagle Hatchery.
	Cryopreserved milt from single anadromous male.
	Captured five residual sockeye salmon adults (four males, one female) in Redfish Lake - held for spawning.
	Developed first residual sockeye salmon broodstock.
1993	Captured eight anadromous adults (6 males, two females) at Redfish Lake Creek trap - held for spawning.
	Captured 48 wild sockeye salmon outmigrants at Redfish Lake Creek trap - transferred to Eagle Fish Hatchery.
	Captured 18 residual sockeye salmon adults (16 males, two females) in Redfish Lake - all females and 12 males contributed to 1993 spawning at Eagle Hatchery.
	Developed 1993 broodstocks and production groups using anad. adults, residual adults, and outmigrants captured in 1991.
	Cryopreserved milt from anadromous, residual, and outmigrant adults.
	First release of pre-spawn adults from broodstock program to Redfish Lake (20 adults released).
	Broodstock nutrition workshop organized and held.
1994	Captured one anadromous female adult at Redfish Lake Creek trap - held for spawning.
	Developed 1994 broodstocks and production groups using anadromous female, outmigrants, and F1 progeny from BY1991.
	First release of hatchery-produced juvenile sockeye to Redfish Lake (~ 14,200 fish).
	Second release of pre-spawn adults from broodstock program to Redfish Lake (65 adults released).
	Developed chilled water capability at Eagle Fish Hatchery - maturing adults held on chilled water.
1995	No anadromous adults captured (no returns).
	Captured 3 residual sockeye salmon adults (three males) in Redfish Lake - held for spawning.
	Developed 1995 broodstocks using wild and F1 residual sockeye salmon adults and mature outmigrants.
	Released approximately 85,000 pre-smolts to Redfish Lake using three release strategies.
	First release of approximately 9,000 pre-smolts to Pettit Lake.
	Estimated that approximately 850 hatchery-produced smolts emigrated from Redfish Lake (overwintered fish).
	First release of approximately 3,800 hatchery-produced smolts to Redfish Lake Creek.
	Kokanee fishery re-opened in Redfish Lake to encourage harvest (competition issue).
1996	Captured one anadromous female adult at Redfish Lake Creek trap - held for spawning.
	Developed 1996 broodstocks and production groups using wild female, and F1 progeny from BY93.
	First use of cryopreserved milt to produce unique broodstocks.
	Released approximately 2,000 pre-smolts to Redfish Lake.
	First plant of eyed-eggs (~105,000) in Redfish Lake.
	Third release of pre-spawn adults to Redfish Lake (120 adults, ~ 30 redds identified).
	Estimated that approximately 14,900 hatchery-produced smolts emigrated from Redfish and Pettit lakes (overwintered fish).
	Released approximately 11,500 smolts in Redfish Lake Creek.
1997	No anadromous adults captured (no returns).
	Developed 1997 broodstocks using F1 progeny from BY1994 and cryopreserved milt from unique males.
	Released approximately 250,000 pre-smolts to three lakes (first year for Alturas Lake release).
	Fourth release of pre-spawn adults (Redfish 80, Alturas 20, Pettit 20, - redds observed in Redfish and Alturas ~ 30 total).
	Second eyed-plant (85,000 to Redfish lake, 20,000 to Alturas Lake).
	Estimated that approximately 400 hatchery-produced smolts emigrated from Redfish Lake (overwintered fish).
1998	Captured one anadromous male adult at Redfish Lake Creek trap - held for spawning.
	Developed 1998 broodstocks and production groups using F1 progeny from BY96, the anad. male, and cryopreserved milt.
	Released approximately 142,000 pre-smolts to three lakes.
	Released approximately 82,000 smolts to Redfish Lake Creek and the upper Salmon River.
	Estimated that aporoximately 58,400 hatchery-produced smolts emigrated from Stanley Basin lakes (overwintered fish).
1999	First hatchery-produced adult return year - Captured seven adult sockeye at Sawtooth Hatchery weir - held one female and three males for spawning and released three males to spawn in Redfish Lake. No wild adults returned.
	Developed 1999 broodstock and production groups using anad. adults, BY96 adults, BY97 adults, and cryopreserved milt.
	Released approximately 41,000 pre-smolts to three lakes.
	Released approximately 10,000 smolts to Redfish Lake and upper Salmon River.
	Third plant of eyed-eggs (20,311 to Pettit Lake).
	Fifth release of pre-spawn adults (21 adults to Redfish Lake).
	Estimated that approximately 33,026 hatchery-produced smolts emigrated from Stanley Basin lakes (overwintered fish).
2000	Second hatchery-produced adult return year - 257 adults returned to Sawtooth and Redfish Lake Creek weirs (243 captured).
	Developed 2000 broodstock and production groups using anad. adults, BY97 and BY98 adults.
	Released approximately 72,000 pre-smolts to three lakes.
	Released 148 smolts to Redfish Lake Creek.
	Fourth plant of eyed-eggs (65,200 to Pettit Lake).
	Sixth release of pre-spawn adults (Redfish 166, Alturas 77, Pettit 28 - redds observed in Redfish and Alturas, >30 total).
	Estimated that approximately 13,000 hatchery-produced smolts emigrated from Stanley Basin lakes (overwintered fish).
2001	Lower Granite dam adult sockeye salmon count (oberservations & video) at the time of this writing = 39.
	Planted approximately 43,000 BY00 sockeye salmon pre-smolts in Redfish Lake net pens.
	Released approximately 14,000 BY99 smolts in Redfish Lake Creek.

Section 3. Relationships to other projects

Project ID	Title	Description
199204000	Redfish Lake Sockeye Salmon Captive Broodstock Rearing and Research	This NMFS project complements IDFG Project 199107200 by sharing fish culture responsibility for the production of captive broodstocks and production eggs. This project serves to also reduce the risk of catastrophic loss of this valuable gene pool.
199305600	Assessment of Captive Broodstock Technology	This NMFS project develops information needed to overcome some of the problems that limit the development of viable broodstock adults and progeny.
199107100	Snake River Sockeye Salmon Habitat and Limnological Research	This Shoshone-Bannock project evaluates nursery lake habitat conditions encountered by juvenile sockeye salmon during their freshwater rearing phase. This information is used to develop annual stocking recommendations from the captive broodstock program.
199009300	Genetic Analysis of Oncorhynchus Nerka	This University of Idaho project provides comprehensive genetics support to Project 199107200.
199700100	Captive Rearing Project for Salmon River Chinook Salmon	This IDFG project shares facility resources and personnel with Project 199107200. Project responsibilities overlap and complement each other.

Section 4. Budget for Planning and Design phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2002 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Planning and Design phase

Section 5. Budget for Construction and Implementation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2002 cost
1. Develop captive broodstocks from Redfish Lake anadromous sockeye salmon. Culture broodstocks.	A. Maintain facilities to produce sockeye salmon captive broodstocks.	ongoing	$155,349
	B. Modify facilities (hatchery building well field) to meet water demands and life support system safety requirements.	ongoing	$57,000
	C. Use existing and emerging conservation hatchery technologies to develop, culture, and maintain sockeye salmon captive broodstocks.	ongoing	$189,750
	D. Trap returning anadromous adults, juvenile out-migrants, and residual sockeye salmon to initiate broodstocks.	ongoing	$25,170
	E. Collect samples for genetic analysis from all wild and hatchery-produced sockeye salmon. Transfer samples to NMFS and University of Idaho cooperators for analysis.	ongoing	$11,731
	F. Incorporate wild and hatchery-produced fish in captive breeding design based on lineage and genetic identity.	ongoing	$6,558
	G. Establish spawning matrices in consultation with NMFS and the program technical oversight committee.	ongoing	$6,558
	H. Spawn captive adults using a factorial design that follows the approved spawning design. Maximize genetic diversity when possible.	ongoing	$10,660
	I. Produce genetically defined progeny (eyed-eggs, pre-smolts, smolts, and adults) for use in multiple release strategies to Stanley Basin lakes.	ongoing	$10,660
	J. Produce genetically defined progeny to meet future broodstock spawning needs. Divide broodstock eggs between NMFS and IDFG facilities for rearing.	ongoing	$10,660
	K. Produce "designer broodstocks" from cryopreserved milt to broaden the genetic base in future spawn years. Divide eggs between NMFS and IDFG facilities for rearing.	ongoing	$10,660
	L. Cryopreserve milt from specific wild and hatchery-produced sockeye salmon.	ongoing	$14,503
	M. Conduct fertilization trials using cryopreserved milt from captive broodstock adults.	ongoing	$8,990
	N. Maintain cryopreserved archives at three locations to spread the risk of loss from catastrophic events.	ongoing	$8,990
	O. Monitor and adaptively manage hatchery outcomes related to fish survival, maturation rates, age-at-maturity, sex ratio, diet, and gamete quality of captive sockeye salmon.	ongoing	$24,338
	P. Evaluate and adaptively manage time held on chilled water (maturing adults) in relation to gamete quality, fertilization rates, and anomalies in broodstock progeny.	ongoing	$7,690
2. Determine the contribution hatchery-produced sockeye salmon make toward recovery.	A. Maintain and operate juvenile and adult trapping facilities at the Sawtooth Hatchery and at lake outlet locations.	ongoing	$36,660
	B. PIT tag wild and hatchery-produced smolts to facilitate evaluations of out migration and adult return success by release location and strategy.	ongoing	$27,978
	C. Differentially fin clip hatchery-produced smolts to facilitate evaluations of out migration and adult return success by release location and strategy.	ongoing	$16,510
	D. Estimate O. nerka out-migration from Stanley Basin lakes by release location and release strategy.	ongoing	$25,512
	E. Evaluate out-migration success by release location and release strategy and adaptively manage the development of future release designs.	ongoing	$28,347
	F. Develop estimates of travel time to lower Snake River hydropower projects and evaluate survival by release location and release strategy.	ongoing	$17,009
	G. Identify spawning location and timing for pre-spawn adult sockeye released to lakes. Estimate spawning success.	ongoing	$7,784
3. Describe O. nerka population characteristics for Stanley Basin lakes in relation to carrying capacity and broodstock program supplementation efforts.	A. Estimate O. nerka population variables by mid-water trawl in four Stanley Basin lakes.	ongoing	$8,105
	B. Trawl sufficiently to estimate abundance and density by age-class.	ongoing	$17,482
	C. Collect scale and otolith samples from trawl captures for age and microchemistry analysis. Take tissue samples for genetic analysis. Take stomachs for diet analysis.	ongoing	$6,398
	D. Develop lake carrying capacity estimates cooperatively with Shoshone-Bannock Tribes.	ongoing	$5,012
	E. Monitor sport fisheries in sockeye salmon nursery lakes to determine their impact on recovery efforts (emphasis on kokanee harvest).	ongoing	$35,083
4. Determine the origin of wild and broodstock O. nerka to provide maximum effectiveness in their utilization within the broodstock program.	A. Use otolith microchemistry to identify the origin of O. nerka with unknown life histories.	ongoing	$12,701
	B. Integrate microchemistry results with genetic information.	ongoing	$3,296
5. Technology Transfer	A. Participate in technical oversight committee process.	ongoing	$18,445
	B. Network with technical experts on issues related to culture and broodstock techniques, genetics, pathology, and monitoring and evaluations.	ongoing	$6,472
	C. Develop cooperative program management plan.	ongoing	$2,965
	D. Coordinate public information transfer with project cooperators.	ongoing	$2,516
	E. Provide written activity reports to satisfy the needs and requirements of IDFG, the technical oversight committee, NMFS, and BPA.	ongoing	$15,687

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost
1. Develop captive broodstocks from Redfish Lake anadromous sockeye salmon. Culture broodstocks.	2003	2006	$2,743,561
2. Determine the contribution hatchery-produced sockeye salmon make toward recovery.	2003	2006	$723,151
3. Describe O. nerka population characteristics for Stanley Basin lakes in relation to carrying capacity and broodstock program supplementation efforts.	2003	2006	$326,208
4. Determine the origin of wild and broodstock O. nerka to provide maximum effectiveness in their utilization within the broodstock program.	2003	2006	$72,396
5. Technology Transfer	2003	2006	$208,564

Outyear budgets for Construction and Implementation phase

FY 2003	FY 2004	FY 2005	FY 2006
$1,312,821	$878,470	$922,295	$968,311

Section 6. Budget for Operations and Maintenance phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2002 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Operations and Maintenance phase

Section 7. Budget for Monitoring and Evaluation phase

Task-based budget

Objective	Task	Duration in FYs	Estimated 2002 cost	Subcontractor

Outyear objectives-based budget

Objective	Starting FY	Ending FY	Estimated cost

Outyear budgets for Monitoring and Evaluation phase

Section 8. Estimated budget summary

Itemized budget

Item	Note	FY 2002 cost
Personnel	FTE: 4.4 permanent, 4.3 temporary	$271,900
Fringe		$98,073
Supplies	All operating supplies and expenses	$200,203
Travel	Technical meetings, workshops	$13,750
Indirect		$131,383
Capital	Major equipment and facility modifications	$124,420
PIT tags	# of tags: 6000	$13,500
		$853,229

Total estimated budget

Total FY 2002 cost	$853,229
Amount anticipated from previously committed BPA funds	$0
Total FY 2002 budget request	$853,229
FY 2002 forecast from 2001	$714,102
% change from forecast	19.5%

Cost sharing

Organization	Item or service provided	Amount	Cash or in-kind

Reviews and recommendations

This information was not provided on the original proposals, but was generated during the review process.

REVIEW:
ISRP Preliminary Review (ISRP 2001-9)

Recommendation:
Fundable only if response is adequate

Date:
Sep 28, 2001

Comment:

A response is needed. Reviewers appreciate the sponsor's effort to provide detailed report of project history and results to date!

The program helped to return 257 adult fish to the Stanley Basin in 2000, and many of which were a result of the multi-agency program. Similar results were not realized in 2001. Given the success of other anadromous salmonids in 2001, to what do the sponsors attribute the poor showing of this program?

Direct release of juveniles to the lake seems to produce best results. Are other salmonids stocked in the lake? If so, what are the densities of these fish in fall compared to summer?

You reported a difference in body fat between fish reared at different hatcheries. Are these differences associated with fish released at different locations and times? It appears that best results were from matings at Big Beef Creek that were transferred as eyed-eggs to Bonneville for rearing and then to Stanley Basin for release. What is the suspected reason for that result?

Is there some chance that you are overstocking Redfish Lake? How does dominance of age-0 fish explain the low biomass estimate?

What is the numeric goal for each population? What are the criteria for delisting? What are the benchmarks for either claiming success or failure of the project?

REVIEW:
CBFWA Funding Recommendation

Recommendation:
High Priority

Date:
Nov 30, 2001

Comment:

This project is considered a BASE project by NMFS since it contributed to the baseline survival of sockeye salmon during the generation of the Biological Opinion. Some managers believe the project goals/target could be firmer.

REVIEW:
ISRP Final Review (ISRP 2001-12A)

Recommendation:
Fund

Date:
Dec 21, 2001

Comment:

Fundable, but low priority -- The project sponsors prepared detailed and responsive answers to reviewer questions and comments. The program benefits from the authors' participation in the program.

The ISRP does not question the credentials of the technical oversight panel or of experts brought in to provide input on specific aspects of the program. The ISRP does, however, remain committed to a detailed and rigorous review of this large and expensive program by a team of outsiders directed to address the performance and continuing need for each element of the program.

REVIEW:
NMFS Review

Recommendation:

Date:
Feb 1, 2002

Comment:

Statement of Potential Biological Benefit to ESU
Increase abundance of listed sockeye ESU through captive broodstock and artificial propagation.

Comments
This is the ongoing IDFG portion of the very important sockeye captive broodstock program. It has been remarkably successful in increasing the number of endangered sockeye and moderately successful in restoring anadromous returns of the species to the Salmon River. This project is providing new conservation biology information and testing techniques for preservation of endangered genetic material in living fish in large quantities, every year.

Already ESA Req? No

Biop? Yes

REVIEW:
BPA Funding Recommendation

Recommendation:
A

Date:
Feb 11, 2002

Comment:

Recommend as critical to implement RPA 177. This project is part of the ongoing artificial propagation safety-net program intended to prevent extinction of Snake River sockeye salmon.

BPA RPA RPM:
177

NMFS RPA/USFWS RPM:
600, 176, 177

REVIEW:
NWPPC Funding Recommendation

Recommendation:
Fund

Date:
Apr 19, 2002

Comment:

REVIEW:
BPA Funding Decision

Recommendation:
Fund

Date:
Jun 13, 2002

Comment:

Fund to implement RPA 177.

REVIEW:
NWPPC Funding Recommendation

Recommendation:
Fund

Date:
Sep 20, 2003

Comment:

Waiting on contract renewal. Increases to offset increases associated with indirect costs/benefits/ facility O&M needs, chiller compressor motor, fleet increases, Captive project, issue 12. Response received from sponsor.

REVIEW:
Project Sponsor Request for FY04-05

Recommendation:

Date:
Sep 20, 2003

Comment:

Increases needed to offset increased costs associated with employee salary and benefit rates, vehicle fleet rental rates, boat and motor rental rates, fish transportation truck rental rates, computer equipment rental rates, and utility charges (added one new pumping system and additional chiller capacity). Scheduled replacement of chiller compressor motors.

REVIEW:
NW Power and Conservation Council's FY 2006 Project Funding Review

Funding category:
expense

Date:
May 2005

FY05 NPCC start of year:	FY06 NPCC staff preliminary:	FY06 NPCC July draft start of year:
$825,638	$825,638	$825,638

Sponsor comments: See comment at Council's website