FY 2000 proposal 20056
    
    
    Section 1. Administrative
    
        | Proposal title | Elucidate Traffic Patterns of Ihn Virus in the Columbia River Basin | 
        | Proposal ID | 20056 | 
        | Organization | USGS-BRD, Western Fisheries Research Center (USGS) | 
        | Proposal contact person or principal investigator | 
        | Name | Gael Kurath | 
        | Mailing address | 6505 NE 65th St. Seattle, WA 98115 | 
        | Phone / email | 2065266583 / gael_kurath@usgs.gov | 
        | Manager authorizing this project |  | 
        | Review cycle | FY 2000 | 
        | Province / Subbasin | Mainstem/Systemwide / Systemwide | 
        | Short description | RNase protection technology will be used to survey the genetic types of IHN virus throughout the Columbia Basin, to identify sources of disease outbreaks, and to infer viral traffic patterns in an effort to reduce the impact of IHNV on basin salmonids. | 
        | Target species | Oncorhynchus mykiss, O. tshawytscha, O. nerka | 
    
    Project location
    
        
            | Latitude | Longitude | Description | 
        
    
    Reasonable and Prudent Alternatives (RPAs)
    
        
        
            Relevant RPAs based on NMFS/BPA review:
            
                | Reviewing agency | Action # | BiOp Agency | Description | 
            
         
     
    
    Section 2. Past accomplishments
    
    
    Section 3. Relationships to other projects
    
        | Project ID | Title | Description | 
    
        
        Section 4. Budget for Planning and Design phase
        Task-based budget
        
            | Objective | Task | Duration in FYs | Estimated 2000 cost | Subcontractor | 
        
        Outyear objectives-based budget
        
            | Objective | Starting FY | Ending FY | Estimated cost | 
        
        Outyear budgets for Planning and Design phase
        
        
        Section 5. Budget for Construction and Implementation phase
        Task-based budget
        
            | Objective | Task | Duration in FYs | Estimated 2000 cost | Subcontractor | 
        
        Outyear objectives-based budget
        
            | Objective | Starting FY | Ending FY | Estimated cost | 
        
        Outyear budgets for Construction and Implementation phase
        
        
        Section 6. Budget for Operations and Maintenance phase
        Task-based budget
        
            | Objective | Task | Duration in FYs | Estimated 2000 cost | Subcontractor | 
        
        Outyear objectives-based budget
        
            | Objective | Starting FY | Ending FY | Estimated cost | 
        
        Outyear budgets for Operations and Maintenance phase
        
        
        Section 7. Budget for Monitoring and Evaluation phase
        Task-based budget
        
            | Objective | Task | Duration in FYs | Estimated 2000 cost | Subcontractor | 
        
        Outyear objectives-based budget
        
            | Objective | Starting FY | Ending FY | Estimated cost | 
        
        Outyear budgets for Monitoring and Evaluation phase
        
    
    Section 8. Estimated budget summary
    Itemized budget
    
        | Item | Note | FY 2000 cost | 
            
                | Personnel | 
                One full-time GS9 technician | 
                $32,478 | 
            
            
                | Fringe | 
                WFRC rate 25% | 
                $8,120 | 
            
            
                | Supplies | 
                radioisotope and supplies | 
                $10,000 | 
            
            
                | Operating | 
                fedex charges for collaborators | 
                $400 | 
            
            
                | Capital | 
                one computer dedicated to the database | 
                $2,000 | 
            
            
                | Travel | 
                one meeting and 1-2 field trips | 
                $1,500 | 
            
            
                | Indirect | 
                WFRC rate 38% | 
                $20,709 | 
            
         | $75,207 | 
    
    Total estimated budget
    
        | Total FY 2000 cost | $75,207 | 
            | Amount anticipated from previously committed BPA funds | $0 | 
        | Total FY 2000 budget request | $75,207 | 
            | FY 2000 forecast from 1999 | $0 | 
            | % change from forecast | 0.0% | 
    
    Cost sharing
    
        | Organization | Item or service provided | Amount | Cash or in-kind | 
            
                | USGS-WFRC | 
                .3 FTE of PI | 
                $17,300 | 
                unknown | 
            
            
                | USGS-WFRC | 
                .3 FTE of GS11 technician | 
                $12,180 | 
                unknown | 
            
            
                | USGS-WFRC | 
                annual radiation license | 
                $5,500 | 
                unknown | 
            
            
                | USGS-WFRC | 
                molecular analyst software programs and scanner | 
                $12,000 | 
                unknown | 
            
    
    
    
    Reviews and recommendations
    This information was not provided on the original proposals, but was generated during the review process.
        
            
            Recommendation:
Fund
            Date:
Jun 15, 1999
         
        Comment:
Recommendation:
Fund for one year as proposed.
Comments:
This project focuses on expanding localized information to assess IHNV traffic throughout the Columbia River Basin, using historical and current collections of IHNV isolate to help resource managers avoid risks associated with movements of fish stocks and their pathogens.  The technical background is thoroughly described, collaborative efforts are ongoing and they provide good rationale regarding management application. However, there's no analysis of problem--how prevalent is IHN, what problem does it pose for restoration.  There's no analysis of cost or threat of the virus to restoration.  No new technology is developed here.  The "research" part of this proposed study deals with genetic fingerprinting of IHNV isolates throughout the basin, some on hand and others to be supplied by collaborators.  Fingerprint patterns are then examined to determine the total number of composite genetic types present.   Data will be archived and compared to provide evidence of viral traffic patterns and sources of disease outbreaks. The proposer already demonstrated that virus moved from wild to hatchery, not vice versa, demonstrating important practical value of technique.
Methods. The proposal is to expand a catalog of known types, then map movements of virus types through the basin. The methods are probably competent but the proposer doesn't present much evidence of peer-reviewed publication of her work, but summarizes work in progress. This is innovative research.        
        
            
            Recommendation:
Do Not Fund
            Date:
Aug 20, 1999
         
        Comment:
        
        
            
            Recommendation:
            Date:
Aug 20, 1999
         
        Comment:
Criteria all: Met? yes -         
        
            
            Recommendation:
            Date:
Aug 20, 1999
         
        Comment:
Sounds like pure theoretical research. Unclear what the application is for the recovery of listed species.        
        
            
            Recommendation:
Rank 14
            Date:
Oct 8, 1999
         
        Comment:
Rank Comments:
This innovative, well-written proposal has the potential to improve existing projects and have systemwide significance in reducing the impact of IHNV on basin salmonids.  The technical background is thoroughly described.        
        
            
            Recommendation:
Rank 14
            Date:
Oct 8, 1999
         
        Comment:
This innovative, well-written proposal has the potential to improve existing projects and have systemwide significance in reducing the impact of IHNV on basin salmonids.  The technical background is thoroughly described.        
        
            
            Recommendation:
Do Not Fund
            Date:
Mar 1, 2000
         
        Comment:
[Decision made in 2-2-00 Council Meeting];