| Proposal title | Preserve Salmonid Gametes |
| Proposal ID | 199703800 |
| Organization | Nez Perce Tribe Department of Fisheries Resource Management (NPT) |
| Proposal contact person or principal investigator |
| Name | Robyn Armstrong |
| Mailing address | PO Box 1942, 125 South Mission Street McCall, ID 83638 |
| Phone / email | 2086345290 / robyna@nezperce.org |
| Manager authorizing this project | Silas Whitman |
| Review cycle | FY 2001 Ongoing |
| Province / Subbasin | Mountain Snake / Salmon |
| Short description | Preserve male salmonid gametes through cryogenic techniques in order to maintain genetic diversity in populations with low levels of abundance and at high risk of localized extinction. |
| Target species | |
| Year | Accomplishment |
|---|
| 1997 |
Cryopreserved 189 chinook salmon and 5 steelhead gametes |
| 1998 |
Cryopreserved 296 chinook salmon and 25 steelhead gametes |
| 1998 |
Submitted 1997 annual report to BPA |
| 1998 |
Conducted chinook fertilization trials with cryopreserved versus fresh milt |
| 1998 |
Thawed and fertilized Grande Ronde basin eggs with cryopreserved milt |
| 1998 |
Cryopreserved 101 Grande Ronde captive broodstock chinook salmon male gametes |
| 1999 |
Cryopreserved 335 chinook salmon and 208 steelhead samples |
| 1999 |
Submitted 1998 annual report to BPA |
| 1999 |
Conducted chinook fertilization trials with cryopreserved versus fresh milt |
| 1999 |
Completed genetic analysis on 1998 chinook and 1999 steelhead cryopreserved |
| 1999 |
Four large freezer tanks operational as short and long term repositories |
| 1999 |
Presented project information at the Western Division American Fisheries Society meeting |
| Objective | Task | Duration in FYs | Estimated 2001 cost | Subcontractor |
|---|
| 1. Coordinate salmonid gamete preservation with management agencies |
a. Meet with other agencies who may help with the collection and/or use the cryopreserved gamete samples |
3 |
$11,000 |
|
|
b. Keep current of the best cryogenic technology |
3 |
$8,000 |
Yes |
|
c. Provide annual reports to NMFS summarizing project activities relating to listed salmonids under ESA |
3 |
$7,000 |
|
| 2. Define project goals for salmonid gene banking (5 years of collection per species @ 100 samples per aggregate) |
a. Identify salmonid conservation units that are at high risk of extirpation |
3 |
$8,000 |
|
|
b. Determine salmonid populations for germplasm sampling |
|
$9,000 |
|
|
c. Annually evaluate sample size required per population to preserve a representative sample of genetic diversity |
|
$5,000 |
|
| 3. Apply cryopreservation techniques to salmonid conservation units at low levels of abundance and high risk of extirpation |
a. Apply for needed ESA Section 10 research permits for cryopreservation activities |
3 |
$6,000 |
|
|
b. Utilize sampling protocols and cryopreservation techniques for the collection, preservation, storage and inventory of male salmonid germplasm |
3 |
$66,000 |
|
|
c. Conduct genetic analysis on fish from which sperm was cryopreserved |
3 |
$20,000 |
Yes |
| 4. Transfer of technology |
a. Provide quarterly and annual reports summarizing all activities associated with cryopreservation of salmonid gametes |
3 |
$22,000 |
|
|
b. Educate agencies, Tribes, public and other professional biologist about the salmonid gamete preservation program |
3 |
$15,000 |
|
Indirect costs have increased about 4% from 2000. Germplasm repository operation and maintenance has increased 50% from 2000 as steelhead sample preservation has increased. Genetic analysis costs have also increased. Cryopreserving gametes from bull trout will incur additional minor costs for the universities. Some support staff were also added.
The scope of this project has increase to cover cutthroat trout.
This information was not provided on the original proposals, but was generated during the review process.
Germplasm repository O&M has increased 50% from 2000 as steelhead sample preservation has increased. Genetic analysis costs have also increased. Cryopreserving gametes from bull trout will incur additional minor costs for the universities. Some support staff were also added. The scope of this project has increased to cover cutthroat trout.