FY 2001 Ongoing proposal 199902200
Contents
Section 1. General administrative information
Section 2. Past accomplishments
Section 3. Relationships to other projects
Section 4. Budgets for planning/design phase
Section 5. Budgets for construction/implementation phase
Section 6. Budgets for operations/maintenance phase
Section 7. Budgets for monitoring/evaluation phase
Section 8. Budget summary
Reviews and Recommendations
Additional documents
| Title | Type |
|---|
Section 1. Administrative
| Proposal title | Assessing Genetic Variation Among Columbia Basin White Sturgeon Populations |
| Proposal ID | 199902200 |
| Organization | University of Idaho (UI) |
| Proposal contact person or principal investigator | |
| Name | Madison Powell |
| Mailing address | 3059F National Fish Hatchery Road Hagerman, ID 83332 |
| Phone / email | 2088379096 / fishdna@micron.net |
| Manager authorizing this project | Madison Powell |
| Review cycle | FY 2001 Ongoing |
| Province / Subbasin | Systemwide / Systemwide |
| Short description | Assessing genetic variation and stock structure among white sturgeon populations in the Columbia Basin using analyses of mitochondrial and nuclear DNA. |
| Target species | |
Project location
| Latitude | Longitude | Description |
|---|
Reasonable and Prudent Alternatives (RPAs)
Sponsor-reported:
| RPA |
|---|
Relevant RPAs based on NMFS/BPA review:
| Reviewing agency | Action # | BiOp Agency | Description |
|---|
Section 2. Past accomplishments
| Year | Accomplishment |
|---|---|
| 1998 | Began examination of white sturgeon genetic diversity as a subcontract to ODFW. A copy of this preliminary report was submitted as section F of the annual report for BPA project number 86-50. |
| 1999 | Completed tasks associated with year one of project, examination of mitochondrial d-loop length variation, and submitted annual report to BPA. |
| 1999 | Preliminary genetic information was presented at either professional meetings or White Sturgeon Work Group meetings during each quarter. |
| 1999 | Organized and held a White Sturgeon Symposium during the Western Division Meeting of the American Fisheries Society. |
| 2000 | Began second year objectives for this project, DNA sequencing of the mitochondrial d-loop region. |
Section 3. Relationships to other projects
| Project ID | Title | Description |
|---|
Section 4. Budget for Planning and Design phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2001 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Planning and Design phase
Section 5. Budget for Construction and Implementation phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2001 cost | Subcontractor |
|---|---|---|---|---|
| 1. Survey available (published and unpublished) sturgeon microsatellite loci for variability. | a. Survey 8, published microsatellite primer sets for variability among 60 samples from 5 sturgeon populations. | .25 | $34,011 | |
| 1. | b. Survey up to 15 additional microsatellite primer sets (unpublished) for their utility and variability among the same sample set above. | .30 | $40,813 | |
| 2. Employ microsatellite analysis on available sturgeon DNA samples. | a. Analyze available sturgeon DNA samples from 24 Columbia Basin populations with variable microsatellite primers. | .33 | $44,894 | |
| 2. | b. Analyze available sturgeon DNA samples from 3 out-of-Basin populations using the same variable microsatellite loci from above to add geographic relevance. | .12 | $16,325 |
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Construction and Implementation phase
Section 6. Budget for Operations and Maintenance phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2001 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Operations and Maintenance phase
Section 7. Budget for Monitoring and Evaluation phase
Task-based budget
| Objective | Task | Duration in FYs | Estimated 2001 cost | Subcontractor |
|---|
Outyear objectives-based budget
| Objective | Starting FY | Ending FY | Estimated cost |
|---|
Outyear budgets for Monitoring and Evaluation phase
Section 8. Estimated budget summary
Itemized budget
| Item | Note | FY 2001 cost |
|---|---|---|
| Personnel | FTE: Principle Investigator: 0.5 FTE Research Support Scientist: 1.0 FTE Administrative Asst.: 0.25 FTE | $69,867 |
| Fringe | Principle Investigator: @0.285 Research Support Scientist @0.25 Administrative Asst. @0.345 | $18,995 |
| Supplies | Chemicals, pipet tips, tubes, labelled primers etc. | $21,850 |
| Travel | 1 professional meeting (AFS or ASIH) and white sturgeon workgroup meetings. | $2,000 |
| Indirect | Off campus indirect cost rate @20.7% | $23,331 |
| $136,043 | ||
Total estimated budget
| Total FY 2001 cost | $136,043 |
| Amount anticipated from previously committed BPA funds | $0 |
| Total FY 2001 budget request | $136,043 |
| FY 2001 forecast from 2000 | $152,000 |
| % change from forecast | -10.5% |
Reason for change in estimated budget
An anticipated capital acquisition (an additional PCR thermal cycler) will not be needed for completion of the project. Also, costs of labelled microsatellite primers for automated fragment analysis have decreased since the original budget estimation.
Reason for change in scope
NA
Cost sharing
| Organization | Item or service provided | Amount | Cash or in-kind |
|---|
Reviews and recommendations
This information was not provided on the original proposals, but was generated during the review process.
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